LOCUS       sequence-282005-        3485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1532
FEATURES             Location/Qualifiers
     source          1..3485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagctccc caactacagc cggcagctgc tgcagcagct gtacactctg
       61 tgcaaggagc agcagttctg tgattgcacc atctccattg gtaccattta cttcagggct
      121 cacaagcttg tcctggctgc tgccagcctc ctgttcaaaa ccctgctgga taacacagat
      181 accatctcca tcgatgcatc tgtggtgagc cccgaggagt ttgcgctctt gttggaaatg
      241 atgtacacgg gcaaactacc tgtgggcaag cacaacttct ccaaaatcat ctccttagca
      301 gacagtctac agatgtttga tgtagctgtt agctgcaaaa atcttctgac cagccttgta
      361 aactgctcgg ttcagggtca ggtggtaagg gatgtctctg cgccatcctc agagacattc
      421 agaaaggaac cagagaagcc tcaagtagaa atcctttcat ctgaaggtgc tggagagcct
      481 cattcttccc cagagcttgc tgccactcca gggggccctg tgaaagctga gactgaggaa
      541 gcagcccatt cagtttcaca agagatgagt gtgaattctc ccacagccca ggagagccag
      601 aggaatgcag aaaccccagc ggagactcct actacagctg aagcttgttc cccctcccct
      661 gctgtgcaaa cctttagtga ggcaaagaag acaagcacag aaccaggatg tgaaaggaaa
      721 cactaccagc tgaattttct tctagaaaat gaaggtgtct tctcagatgc actcatggtt
      781 acccaggatg ttttaaaaaa actagaaatg tgttcagaaa ttaaaggtcc acagaaggag
      841 gtgattctga attgctgtga gggcagaaca cccaaggaga caatagaaaa tttgttgcac
      901 agaatgactg aagagaagac gctgactgct gagggtttgg taaaactcct ccaggctgtg
      961 aagacgactt tcccaaacct gggccttctg ctagagaagt tgcagaaatc agccactttg
     1021 ccaagcacca cagtccaacc aagccctgat gattatggga ctgagctatt gagacgctat
     1081 catgaaaacc tctctgagat tttcacagac aaccagattt tattaaagat gatctcacac
     1141 atgacaagtt tagcccctgg agaaagagag gtcatggaga agcttgtgaa acgtgactct
     1201 ggttcaggtg gtttcaattc tctgatatca gcagttctag aaaagcagac tctctctgcc
     1261 acagccattt ggcaactgct gctggtggtt caggagacaa agacctgtcc attggacctg
     1321 ctcatggagg aaatacgaag ggagcctggt gccgatgctt tcttccgggc agtgaccacc
     1381 ccagaacatg ccactttaga aacaatcctg aggcataacc agttgatctt ggaggccatc
     1441 caacagaaga ttgagtacaa gctctttacc tcggaggagg agcacctggc agagactgtg
     1501 aaagagattc tgagcattcc ctctgagaca gccagccctg aagcttccct gagagcagtg
     1561 ctgagcagag ccatggaaaa atcagtcccg gccattgaaa tatgccacct cctgtgcagt
     1621 gtccacaaat cttttccagg cctgcagcct gtcatgcagg agttggcata cattggtgtc
     1681 cttactaagg aagatggaga gaaggaaacg tggaaggtga gtaataaatt tcaccttgaa
     1741 gccaacaaca aagaagatga aaaggcagcc aaagaagaca gccagcctgg ggaacagaat
     1801 gatcaaggag agactggttc cttgccagga cagcaagaga aagaggcttc agcctcccca
     1861 gaccctgcca agaagagctt catctgtaag gcctgcgaca aaagcttcca tttctactgc
     1921 cgcctaaagg tgcacatgaa gcgctgccgg gtggctaaga gcaaacaggt gcagtgtaag
     1981 gagtgcagtg agaccaagga ttcaaagaaa gagctggaca aacatcagct ggaggcccat
     2041 ggtgcaggtg gagagcccga tgcccccaag aagaagaaga agaggcttcc agtgacatgt
     2101 gacctctgtg gcagagaatt tgcccatgcc tcaggcatgc agtaccataa gctgacagag
     2161 cacttcgatg agaagccttt ctcctgtgaa gagtgtgggg cgaagtttgc agccaattcc
     2221 accctgaaga accaccttcg ccttcacacc ggggaccgcc cgttcatgtg caagcactgc
     2281 ctcatgacct tcacccaggc ctccgccctg gcctatcaca ccaagaagaa gcactcagaa
     2341 gggaaaatgt atgcatgcca gtactgtgat gctgtgtttg cccagtctat tgagctgtcc
     2401 cgccacgtga ggacccacac cggggacaag ccctatgtct gcagagactg tggcaagggc
     2461 ttccggcaag ccaatggcct ctccatccat ctgcacacct ttcacaacat agaagatcct
     2521 tatgactgca agaagtgcag gatgagtttc cccactcttc aggatcaccg gaagcacatc
     2581 catgaggtgc actccaaaga gtaccacccc tgccccacgt gtgggaagat cttcagtgcc
     2641 ccgtccatgc tggagcggca cgtggtgacc cacgttggag ggaagccctt cagctgcggg
     2701 atctgcaaca aggcctacca gcaattgtct ggtttgtggt accacaatcg aacccaccac
     2761 cctgacgtat ttgctgctca gaaccaccga tcttccaagt tctcatcact ccagtgcagc
     2821 tcctgtgaca aaaccttccc caacaccatt gagcacaaga agcacatcaa agcagaacat
     2881 gcagatatga agttccatga atgtgaccag tgtaaggagc tcttccccac gccagccttg
     2941 ctgcaggttc atgtcaagtg ccagcattca gggtcccagc cattccggtg cttgtactgt
     3001 gctgctactt tccgttttcc tggagcattg cagcaccatg tcaccacgga gcacttcaag
     3061 cagtcagaga ccaccttccc ctgtgagctc tgtggggaac tcttcacctc ccaggcccag
     3121 cttgacagtc acctggaatc tgagcaccca aaggtgatga gcacggaaac ccaggccgca
     3181 gcctcacaga tggcgcaggt gatccaaacc ccagagccgg tggccccgac agagcaggtg
     3241 atcactttgg aggagaccca gcttgccggg tcgcaggtgt ttgtgacgtt gccagattct
     3301 caggcatctc aggccagctc tgagctcgtg gcggtgactg tggaggactt gctggatggc
     3361 acagtgacgc tgatctgtgg tgaggccaaa tagtaatgaa ctagtccgta acttgaaagt
     3421 atttcgattt cttggcttta tatatcttgt ggaaaggacg aacacaatgt agagcagatt
     3481 ggtta
//
