LOCUS       sequence-282008-        2345 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1539
FEATURES             Location/Qualifiers
     source          1..2345
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggccgcct gaacgagcag cgcctcttcc agcctgacct ctgcgacgtg
       61 gacttggtgc tggtgcccca gcgcagcgtc tttccggcac acaagggtgt gctagccgcc
      121 tacagccagt tcttccactc actcttcacc cagaacaagc agctgcagcg tgtggagctg
      181 tccctggagg cactggcacc tggtggcctg cagcagatcc tcaacttcat ctatacgtcc
      241 aagctgctgg tcaacgcggc caacgtccac gaggtgctca gcgccgcctc attgctgcag
      301 atggctgaca tcgctgcgtc ctgccaagag ctgctggacg cccgctctct aggcccacca
      361 ggtccgggca ctgtggccct ggcccagccg gctgccagct gcactccagc tgcgccgccc
      421 tactactgtg acatcaagca ggaggccgac accccaggcc tgcccaagat ctatgcccgc
      481 gagggccctg acccttactc ggtgcgtgtt gaggacgggg cagggactgc tggtggcaca
      541 gtgcctgcca ccattgggcc agcccagccc ttctttaagg aggagaagga gggtggtgtc
      601 gaggaggccg gtgggccccc agccagcttg tgcaagctgg agggtggaga agagttggag
      661 gaagagcttg ggggttctgg cacctacagc cgcagggagc aatcccagat catcgtggag
      721 gtgaacctca acaaccagac actgcacgtg tccacggggc cagaggggaa gccaggtgcc
      781 gggccaagcc cagccaccgt ggttctgggc cgggaggacg ggctgcagag acactcggac
      841 gaggaggagg aggacgacga ggaggaggag gaggaagaag aggaagagga aggtggtggc
      901 agtggacggg aggaggagga ggaggaagag ggtggcagtc agggagaaga ggaagaagag
      961 gaggaggacg ggcacagtga gcaggaagag gaagaggagg aggaagagga ggaagggcct
     1021 agtgagcagg atcaagagag ctctgaggag gaggaggggg aggaggggga ggctgggggc
     1081 aagcaggggc cacggggaag ccgaagcagc cgggcagacc cccctcccca cagtcacatg
     1141 gccacacggt cccgggagaa cgcccggcgc cggggtaccc ctgaacctga agaagctggg
     1201 cggcggggtg ggaagaggcc aaagccaccc cctggagtgg cctctgcatc ggcccgaggg
     1261 ccgccagcca ctgatgggct gggggccaag gtgaagctgg aggagaagca gcaccatcca
     1321 tgccagaagt gcccacgagt tttcaacaac cgctggtacc tggagaaaca catgaatgtg
     1381 acccacagcc gcatgcagat ctgcgaccag tgcggcaagc gcttcctgct ggagagcgag
     1441 ctgctgctgc acaggcagac agactgcgag cgcaacatcc agtgtgtgac atgtggcaaa
     1501 gctttcaaga agctttggtc cctccatgag cataacaaga ttgtgcacgg ctacgcagag
     1561 aagaagttct catgcgagat ctgtgagaag aagttctaca ccatggccca cgtgcgtaag
     1621 cacatggttg cccacaccaa ggacatgccc ttcacctgcg agacctgcgg aaagtccttc
     1681 aagcgcagca tgtccctcaa ggtgcactca ctgcagcact caggggagaa gccgttcaga
     1741 tgtgagaact gcaatgagcg cttccagtac aagtaccagc tgcggtcaca catgagcatc
     1801 cacattggcc acaagcagtt catgtgccaa tggtgcggca aggacttcaa catgaagcag
     1861 tacttcgatg agcacatgaa gacccacaca ggagagaagc cgtacatctg cgagatctgt
     1921 ggcaagagct tcaccagccg gcccaacatg aagcggcacc ggcgcacgca cacgggcgag
     1981 aagccgtacc cgtgcgacgt gtgtggccag cgcttccgct tctccaacat gctcaaggcc
     2041 cacaaggaga agtgcttccg cgtcagccac accctggccg gcgacggcgt ccccgctgcc
     2101 ccaggcctgc ccccaaccca gccccaggcg cacgcactgc ccctgctccc ggggctgccc
     2161 cagaccctgc cgcccccgcc ccacctgccg cccccgcctc cgctcttccc caccactgcc
     2221 agccccggcg ggaggatgaa cgccaacaac tagtaatgaa ctagtccgta acttgaaagt
     2281 atttcgattt cttggcttta tatatcttgt ggaaaggacg aattaaacgg gattccaagg
     2341 ccacg
//
