LOCUS       sequence-282026-        1325 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1575
FEATURES             Location/Qualifiers
     source          1..1325
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagctaa aatggagata actttaagct ccaacactga agcttcctcc
       61 aagcaagaga gacacataat agccaaacta gaagagaaac ggggccctcc tctgcaaaaa
      121 aactgcccag atcctgagct ctgccgccag agcttcagac gcttttgtta tcaagaggtg
      181 tctggacccc aagaggctct ctcccagctc cgacagctct gccgtcagtg gttgcaaccc
      241 gagctgcaca ccaaggagca gattttggag cttctggtga tggagcagtt cctgaccatc
      301 ctgcccccgg agatccaggc tcgggtcagg catcgatgtc caatgagcag caaggagatt
      361 gtgaccctcg tggaagattt tcacagagca tccaagaaac caaagcagtg ggtggccgtt
      421 tgtatgcagg ggcaaaaggt gctcttggag aaaactggat ctcagcttgg agaacaggaa
      481 ctgccagact ttcaaccgca gactcctagg agagatctca gggagagctc tccagcagag
      541 ccttcccagg caggagctta tgaccggctg agcccccatc attgggagaa atccccactc
      601 ctccaagaac caacccccaa attggctggg acagaggccc ccagaatgag aagtgacaac
      661 aaggaaaatc cacaacagga aggggctaaa ggagcaaagc catgtgcagt gtcagctggc
      721 agatccaaag ggaatggtct gcagaatcct gaaccaagag gggcaaatat gagtgaacct
      781 cggttgtcac ggaggcaggt cagctcccca aatgctcaaa agccatttgc tcactaccag
      841 agacattgca gggtggaata catcagcagc cccctaaaaa gccacccact gagagagcta
      901 aagaaaagca aaggaggtaa acggagtctg agcaaccgtt tgcaacatct tggtcaccag
      961 cccacccgct cagcaaagaa accctacaaa tgtgatgact gtgggaaaag cttcacgtgg
     1021 aattcagagc tgaagagaca caagagagtc cacacaggag agagacccta cacgtgcgga
     1081 gagtgtggaa actgctttgg gcggcagtca accctgaagc tgcaccagag gatccacact
     1141 ggagagaagc cataccagtg tggccagtgt gggaaaagct ttcgccagag ctcaaacctt
     1201 caccagcatc accgacttca ccatggggac tagtaatgaa ctagtccgta acttgaaagt
     1261 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaccatggga cggtctttca
     1321 cgtga
//
