LOCUS       sequence-282029-        1547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1576
FEATURES             Location/Qualifiers
     source          1..1547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgcagg gtggctgaca acctggtcac agaactcagt aaccttccag
       61 gaagtggcag tggacttttc ccaggaggag tgggcattgc tggaccctgc tcaaaaaaat
      121 ctatacaaag atgtgatgct ggagaacttt aggaacctgg cctcagtagg gtatcagctc
      181 tgcagacaca gtctgatctc caaggtggat caagaacagc tgaagacaga tgaaagagga
      241 attttacaag gtgactgtgc agactgggaa actcaactta aaccaaaaga tacaattgct
      301 atgcagaaca ttcctggggg aaaaacatcc aatggcataa acacggcaga aaatcaacct
      361 ggtgagcact ccctggagtg taaccattgt gggaaattca gaaagaacac tcgctttatt
      421 tgtacaagat attgcaaggg agagaaatgc tataaatata taaagtatag caaagtcttc
      481 aaccatccct caactcttag gagtcatgtg agcattcaca ttggagagaa aactcttgaa
      541 tttactgatt gtagaaaagc tttcaatcaa gagtcatccc tcaggaaaca cttaagaact
      601 cccacaggac agaagtttca ggagtatgag caatgtgata tgtccttcag cctacactct
      661 tcctgctcag tacgtgagca aatacctact ggagagaaag gtgatgaatg cagtgactat
      721 ggcaaaatat ctccccttag tgtccacaca aaaactggta gtgtggagga gggtttggaa
      781 tgtaatgaac atgagaaaac tttcactgac cctttgtccc ttcagaactg tgtcagaact
      841 cactctggag agatgcccta tgaatgcagt gactgtggga aagccttcat ttttcagtct
      901 tcccttaaga aacacatgag atctcatact ggagagaagc cttatgagtg tgatcactgt
      961 ggaaaatcct ttagccagag ctctcatctg aatgtgcaca aaagaactca cactggagag
     1021 aaaccctatg actgtaagga atgtgggaag gctttcactg ttccttcatc ccttcagaaa
     1081 catgtgagaa cccacactgg agagaaaccc tatgaatgca gtgactgtgg aaaagccttc
     1141 atcgatcagt catcccttaa gaaacacaca cgctctcaca ctggagagaa gccttatgag
     1201 tgtaaccagt gtggaaagtc cttcagcaca ggctcttacc ttattgtgca caagagaact
     1261 cacactggtg agaaaaccta tgagtgtaaa gaatgtggga aggcctttag gaattcctct
     1321 tgcctgaggg tacacgtgag aactcacact ggagagaagc cttataaatg tattcagtgt
     1381 gaaaaagcct ttagcacaag cactaacctt ataatgcaca agcgaatcca caatggccag
     1441 aaactccatg aatagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1501 tatatatctt gtggaaagga cgaccaggct gctactgaac cgcgtag
//
