LOCUS       sequence-282032-        1235 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1587
FEATURES             Location/Qualifiers
     source          1..1235
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggatga agagatacct tttgagcaga gcttttctat aggaatgtca
       61 cagatcagga ttcccaaggg aggtccttct actcagaagg cttacccctg tgggacatgt
      121 ggcctggtct taaaagacat tttgcacttg gctgagcacc aggaaacaca cccagggcag
      181 aaaccataca tgtgtgtgct gtgtgggaaa cagttctgct tcagtgcaaa ccttcaccag
      241 caccagaagc agcacagtgg agagaaaccc tttagaagtg ataagagcag gccctttctt
      301 ctgaacaact gtgctgtgca atcaatggag atgtcttttg tgacagggga ggcttgtaag
      361 gacttcctag ccagctcaag cattttcgag caccatgccc ctcacaatga gtggaagcca
      421 cacagcaaca ccaagtgtga ggaggcctct cactgtggaa aaaggcatta caaatgcagt
      481 gaatgtggga aaaccttcag ccgcaaagac tcacttgttc aacaccagag agtccacact
      541 ggagaaaggc cttatgagtg cggtgaatgt gggaaaacct ttagccgcaa acccatactt
      601 gctcagcacc agagaatcca cactggagaa atgccttatg agtgtggcat atgtgggaaa
      661 gtttttaatc atagctctaa ccttattgta catcaaagag tacacactgg agcaaggcct
      721 tacaagtgca gtgaatgtgg gaaagcctat agtcacaaat ctacacttgt tcagcatgag
      781 agtatccata ctggagaaag gccatatgag tgcagcgaat gtggaaaata ctttggtcac
      841 aaatacagac tcattaaaca ttggagtgtt catactggag caaggcctta tgagtgcatc
      901 gcatgtggga agttctttag ccaaagctct gaccttattg cacatcaaag agttcataat
      961 ggtgaaaagc cttatgtgtg cagtgaatgt ggaaaagcct ttagccacaa acatgtactt
     1021 gttcagcatc atagaattca cactggagaa aggccatata agtgcagtga atgtgggaag
     1081 gccttcaggc agagggcttc cctcatccgc cactggaaaa ttcacactgg agaaaggcct
     1141 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1201 ggaaaggacg acctttctag cagttctagt actca
//
