LOCUS       sequence-282038-        1427 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1589
FEATURES             Location/Qualifiers
     source          1..1427
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc cacgctgagg gaccccgctc agggctatgt gacctttgag
       61 gacgtggctg tctacttctc ccaggaggaa tggagattgc ttgatgacgc tcagaggctc
      121 ctctaccgca atgtgatgct ggagaacttt acacttctgg cctctctggg acttgcatct
      181 tccaagaccc atgaaataac ccagctggag tcatgggagg agcccttcat gcctgcttgg
      241 gaagttgtga cttcagccat actgagaggt agttggcaag gagccaaggc tgaggcagct
      301 gctgagcaga gtgcttctgt agaagtgccc agttcaaacg ttcagcaaca ccagaagcag
      361 cactgtggag agaaaccctt aaaaagacaa gagggcaggg tcccagtttt gaggagttgc
      421 agagtccacc tatcagagaa gtccttgcaa agcagggaag ttgggaagga tcttctgacc
      481 agctcaggtg ttctcaagca ccaggtgact cacacgggag agaagtcaca taggagctcc
      541 aaaagtaggg aggcctttca tgctggaaaa aggcattaca aatgcagtga atgtgggaaa
      601 gcctttggtc agaaatattt acttgttcag caccagagac tgcacactgg ggaaaagcct
      661 tatgaatgca gtgaatgtgg gaagttattt agccataagt ccaacctttt tatacaccaa
      721 atagttcaca ctggagaaag gccttatggg tgtagtgact gtggaaaatc ctttagccgt
      781 aatgctgacc tcattcaaca ccagagagtt cacactggag aaaagccttt tacatgcagt
      841 gaatgtggaa aagctttcag gcataattcc acacttgttc agcatcacag aatccacact
      901 ggagtaaggc cttatgagtg cagtgaatgt ggaaaattgt ttagtttcaa ctccagcctc
      961 atgaaacatc agagagttca cactggagaa agaccttata agtgcagtga atgtggaaaa
     1021 ttctatagcc acaagtccag ccttatcaat cattggcgtg ttcacactgg agaaaggcct
     1081 tatgagtgca gtgaatgtgg gaaatttttt agccaaagct caagcctcat gcaacatcga
     1141 aaagttcaca ctggagaaaa accttttaag tgcaatgaat gtgggagatt ctttagtgag
     1201 aattccagcc ttgttaaaca tcagagggtt cacactggag caaagcctta tgagtgcagg
     1261 gaatgtggga aattttttcg ccacagctcc agtcttgtta aacatcgaag gattcacact
     1321 ggagaaatac aatagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1381 tatatatctt gtggaaagga cgaagaagaa ctttagccta cctatca
//
