LOCUS       sequence-282041-        1301 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1591
FEATURES             Location/Qualifiers
     source          1..1301
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcctttgt gaccagtgga gaattggttc ggcatcgtcg ttacaaacac
       61 acccacgaga agccattcaa gtgttccatg tgcgattacg ccagtgtaga agtcagcaaa
      121 ttaaaacgtc acattcgctc tcatactgga gagcgtccgt ttcagtgcag tttgtgcagt
      181 tatgccagca gggacacata caagctgaaa aggcacatga gaacccattc aggggaaaag
      241 ccttatgaat gttatatttg tcatgctcgg tttacccaaa gtggtaccat gaagatgcac
      301 attttacaga agcacacaga aaatgtggcc aaatttcact gtccccactg tgacacagtc
      361 atagcccgaa aaagtgattt gggtgtccac ttgcgaaagc agcattccta tattgagcaa
      421 ggcaagaaat gccgttactg tgatgctgtg tttcatgagc gctatgccct catccagcat
      481 cagaagtcac acaagaatga gaagcgcttt aagtgtgacc agtgtgatta cgcttgtaga
      541 caggagaggc acatgatcat gcacaagcgc acccacaccg gggagaagcc ttacgcctgc
      601 agccactgcg ataagacctt ccgccagaag cagcttctcg acatgcactt caagcgctat
      661 cacgacccca acttcgtccc tgcggctttt gtctgttcta agtgtgggaa aacatttaca
      721 cgtcggaata ccatggcaag acatgctgat aattgtgctg gcccagatgg cgtagagggg
      781 gaaaatggag gagaaacgaa gaagagtaaa cgtggaagaa aaagaaagat gcgctctaag
      841 aaagaagatt cctctgacag tgaaaatgct gaaccagatc tggacgacaa tgaggatgag
      901 gaggagcctg ccgtagaaat tgaacctgag ccagagcctc agcctgtgac cccagcccca
      961 ccacccgcca agaagcggag aggacgaccc cctggcagaa ccaaccagcc caaacagaac
     1021 cagccaacag ctatcattca ggttgaagac cagaatacag gtgcaattga gaacattata
     1081 gttgaagtaa aaaaagagcc agatgctgag cccgcagagg gagaggaaga ggaggcccag
     1141 ccagctgcca cagatgcccc caacggagac ctcacgcccg agatgatcct cagcatgatg
     1201 gaccggtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1261 tcttgtggaa aggacgattc aaagaagcgg tgaggcgctg g
//
