LOCUS       sequence-282044-        1883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1617
FEATURES             Location/Qualifiers
     source          1..1883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgcgca caggccggtg gaatgggtcc aggccgtggt cagccgcttc
       61 gacgagcagc ttccaataaa aacaggacag cagaacacac ataccaaagt cagtactgag
      121 cacaacaagg aatgtctaat caatatttcc aaatacaagt tttctttggt tataagcggc
      181 ctcactacta ttttaaagaa tgttaacaat atgagaatat ttggagaagc tgctgaaaaa
      241 aatttatatc tctctcagtt gattatattg gatacactgg aaaaatgtct tgctgggcaa
      301 ccaaaggaca caatgagatt agatgaaacg atgctggtca aacagttgct gccagaaatc
      361 tgccattttc ttcacacctg tcgtgaagga aaccagcatg cagctgaact tcggaattct
      421 gcctctgggg ttttattttc tctcagctgc aacaacttca atgcagtctt tagtcgcatt
      481 tctaccaggt tacaggaatt aactgtttgt tcagaagaca atgttgatgt tcatgatata
      541 gaattgttac agtatatcaa tgtggattgt gcaaaattaa aacgactcct gaaggaaaca
      601 gcatttaaat ttaaagccct aaagaaggtt gcgcagttag cagttataaa tagcctggaa
      661 aaggcatttt ggaactgggt agaaaattat ccagatgaat ttacaaaact gtaccagatc
      721 ccacagactg atatggctga atgtgcagaa aagctatttg acttggtgga tggttttgct
      781 gaaagcacca aacgtaaagc agcagtttgg ccactacaaa tcattctcct tatcttgtgt
      841 ccagaaataa tccaggatat atccaaagac gtggttgatg aaaacaacat gaataagaag
      901 ttatttctgg acagtctacg aaaagctctt gctggccatg gaggaagtag gcagctgaca
      961 gaaagtgctg caattgcctg tgtcaaactg tgtaaagcaa gtacttacat caattgggaa
     1021 gataactctg tcattttcct acttgttcag tccatggtgg ttgatcttaa gaacctgctt
     1081 tttaatccaa gtaagccatt ctcaagaggc agtcagcctg cagatgtgga tctaatgatt
     1141 gactgccttg tttcttgctt tcgtataagc cctcacaaca accaacactt taagatctgc
     1201 ctggctcaga attcaccttc tacatttcac tatgtgctgg taaattcact ccatcgaatc
     1261 atcaccaatt ccgcattgga ttggtggcct aagattgatg ctgtgtattg tcactcggtt
     1321 gaacttcgaa atatgtttgg tgaaacactt cataaagcag tgcaaggttg tggagcacac
     1381 ccagcaatac gaatggcacc gagtcttaca tttaaagaaa aagtaacaag ccttaaattt
     1441 aaagaaaaac ctacagacct ggagacaaga agctataagt atcttctctt gtccatggtg
     1501 aaactaattc atgcagatcc aaagctcttg ctttgtaatc caagaaaaca ggggcccgaa
     1561 acccaaggca gtacagcaga attaattaca gggctcgtcc aactggtccc tcagtcacac
     1621 atgccagaga ttgctcagga agcaatggag gctctgctgg ttcttcatca gttagatagc
     1681 attgatttgt ggaatcctga tgctcctgta gaaacatttt gggagattag gtatatgtac
     1741 ttttattttt taaattcaac ttttaaattt tattttgtat ttttgtctta gtaatgaact
     1801 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     1861 cttagggact ttcgacttca gtt
//
