LOCUS       sequence-282050-        1886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1664
FEATURES             Location/Qualifiers
     source          1..1886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      848..857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagcccc tgtcaaaggg aacaggaagc agtccacgga gggtgacgcc
       61 ctagacccac ctgcatcccc caaacctgct ggcaagcaga acggaatcca gaaccccatc
      121 tcgctggagg actcccccga ggcaggcggg gagcgggagg aggagcagga gcgggaggag
      181 gagcaggcct tcctggtcag cctctacaag ttcatgaagg agcgacacac gcccatcgag
      241 agggtgcccc atctcggctt caagcagatt aacctgtgga agatctacaa agcagtggag
      301 aagctggggg cctatgagct ggtgaccggg cgccgcctct ggaagaacgt gtacgacgag
      361 ctggggggca gcccaggcag caccagcgcg gccacgtgca cgcgccgcca ctacgagagg
      421 ctggtcctgc catacgtgcg gcacctgaag ggggaggatg acaagccgct gcccacctcc
      481 aagcccagga aacagtacaa gatggctaag gagaacaggg gggatgatgg ggccaccgag
      541 aggccgaaga aggccaagga ggagcggcgc atggaccaga tgatgccagg aaagaccaaa
      601 gcagatgctg ctgacccagc accacttccc agccaggagc cccccaggaa cagcacagaa
      661 cagcagggcc tggcctctgg gtcttctgtg tcctttgtgg gtgccagcgg ctgtcctgag
      721 gcctacaagc ggctcctatc cagcttctac tgcaagggga cacacggcat catgtcacca
      781 ctggccaaaa agaagctcct ggcccaggtg agcaaggtgg aggccttgca gtgccaggag
      841 gagggctgcc gccatggggc agagccccag gcgtccccag ctgttcacct cccagagagt
      901 ccccagagcc ccaaagggct gactgagaac tccaggcacc ggctgacccc tcaggaggga
      961 ttgcaggccc caggtggcag cctcagagag gaggcgcagg caggcccctg cccggcagcc
     1021 cccatcttca agggctgctt ctacacccac cccaccgagg tgctgaagcc tgtcagccag
     1081 caccccaggg acttcttctc tagacttaaa gatggggtgc tattggggcc tcctggcaaa
     1141 gaggggctgt cagtgaaaga gccccagctg gtgtggggcg gagacgctaa ccgcccttct
     1201 gcgttccata aaggtggctc cagaaagggc atcctctacc ccaagcccaa agcctgctgg
     1261 gtgtccccca tggccaaggt cccagccgag agccccacgc tcccgcccac cttccccagt
     1321 agcccaggcc tgggcagcaa gcgcagcctg gaggaagagg gtgctgccca cagtgggaag
     1381 agactgcggg ccgtgtctcc ctttcttaag gaggcggatg ccaagaagtg tggggccaaa
     1441 cctgcagggt ccggcctggt ctcctgcctt ctgggcccag ccctggggcc tgtgccccca
     1501 gaggcctaca ggggcaccat gctgcactgc ccgctgaact tcactggcac cccgggcccc
     1561 ttgaagggcc aggctgcact ccccttcagc cccctggtca tcccggcctt cccggcccac
     1621 ttcctggcca ccgcaggccc ctcgcccatg gccgctggcc tgatgcactt ccccccaacg
     1681 tccttcgaca gtgccctccg ccacagactt tgcccggcct catctgcctg gcacgcacca
     1741 ccagtcacaa cctatgcagc gccccacttc ttccacctca acaccaagct gtagtaatga
     1801 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1861 gacctggtaa gcaggacata atttct
//
