LOCUS       sequence-282071-        1823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1738
FEATURES             Location/Qualifiers
     source          1..1823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(626..637)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tgttacagcc tggtcctcat cctccctcac cccaagctgc tgctcctgga
       61 gaagcctggc caggcccctc tcaggctccc tggcagagcc tagaggagaa gatggggagc
      121 cccgaggatg acctgattgg gattccattc ccggaccaca gcagtgagct cctgagctgc
      181 ctcaatgagc agcgccagct gggccaccta tgtgacctca ccatccggac gcagggcctt
      241 gaataccgca cccacagggc tgtgctagct gcctgtagcc actacttcaa gaagcttttc
      301 actgagggcg gtggcggagc tgtcatgggg gccgggggta gcgggacggc cactggggga
      361 gcaggggccg gtgtgtgtga gctggacttt gtagggccag aggcactagg cgccctcctt
      421 gaatttgcct atacagccac actgaccacc agcagcgcca acatgccagc tgtgctccag
      481 gctgcccgcc tgctggagat cccgtgtgtc atcgctgctt gcatggagat tctgcagggc
      541 agtgggctag aagctcccag cccggacgag gatgactgtg agcgagcccg ccagtatctg
      601 gaggcctttg ccacagccac ggcctctgga gttcccaatg gtgaagacag tcctccacag
      661 gtgcccctcc caccacctcc gccaccgcca cctcggcctg ttgcccgccg cagccgcaag
      721 ccccggaaag ctttcctgca aaccaagggg gccagagcaa accacctagt ccctgaggtg
      781 cccacagtgc ccgcccatcc cttgacctat gaggaggagg aggtggcggg cagagtgggc
      841 agcagtgggg gcagtgggcc gggggacagc tacagccctc ccacaggaac tgcctcccct
      901 cctgagggtc cccagagcta cgaaccctat gagggtgagg aagaagaaga ggagctggta
      961 tatcccccag cctatgggct ggcgcagggt ggcgggcccc cgctgtcccc agaggagctg
     1021 ggctcagatg aggatgccat cgatcctgac ctgatggcct acctaagctc cctgcaccag
     1081 gacaacctgg caccaggcct ggacagccaa gacaagctgg tgcgcaaacg ccgctcccag
     1141 atgcctcagg agtgccctgt ctgccacaag atcatccatg gggcaggcaa actgcctcgc
     1201 cacatgagga cccacacagg cgagaagccc tttgcctgcg aggtctgcgg tgttcgattc
     1261 accaggaacg acaagctgaa gatccacatg cggaagcaca cgggagagcg cccctactca
     1321 tgcccgcact gcccagcccg cttcctgcac agctacgacc tcaagaacca catgcacctg
     1381 cacacagggg accggcccta tgagtgccac ctgtgccaca aggctttcgc caaggaggac
     1441 cacctgcagc gccacctcaa aggccagaac tgcctggagg tgcgcacccg acggcgccgc
     1501 aaggacgatg caccacccca ctacccacca ccctctaccg ctgctgcatc ccccgctggc
     1561 ctcgacctct ccaatggcca cctggacacc ttccgcctct ctctagctcg attctgggag
     1621 cagtcagccc ccactgggcc cccggtctct accccagggc cccctgatga cgatgaggag
     1681 gaaggggcac ccaccacacc ccaggctgaa ggtgccatgg agtcctctta gtaatgaact
     1741 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     1801 ctgtcaacga gacaaacaag tcg
//
