LOCUS       sequence-282077-        1643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1763
FEATURES             Location/Qualifiers
     source          1..1643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagttcac ctgggatgaa tggcagctac tggattctac acagaagtac
       61 ctgtacagag atgtgatatt ggaaaactat cataacctga tatcagtggg gtatcatggt
      121 accaagcctg acttaatctt caagttggaa caaggagaag atccatggat aataaatgcc
      181 aaaatttcca ggcagagctg tccagatggc tgggaagaat ggtaccagaa caatcaagat
      241 gagcttgaga gtattgaaag aagctatgct tgtagtgtgt tgggaagact taatctgagc
      301 aaaacccatg attcttcaag acagagactc tataacacac gtggaaaaag tttgacacaa
      361 aactcagctc caagcagaag ttatttaaga aagaatcctg ataagtttca tggttatgaa
      421 gaaccatatt ttcttaagca tcaaagagct catagcatag aaaaaaactg tgtgtgtagt
      481 gaatgtggga aagcttttcg ttgtaagtca cagctcattg tacatctcag aattcataca
      541 ggagagagac cttatgaatg cagtaaatgt gaaagagcct tcagtgccaa gtcaaacctt
      601 aatgctcatc agagagttca tacaggagaa aaaccctact catgtagtga gtgcgagaag
      661 gtcttctctt tcaggtcaca gctcattgtc catcaggaaa ttcacacagg agggaaaccc
      721 tatggctgca gtgaatgtgg gaaagcctac agttggaaat cacagcttct tttacaccag
      781 agaagtcaca caggagtgaa accgtatgaa tgcagcgaat gtgggaaagc ctttagtttg
      841 aagtctccat tcgttgtaca ccagagaact catacaggag tgaaacccca taaatgcagt
      901 gaatgtggga aagcctttag gagtaagtcc tatctccttg ttcacatccg aatgcataca
      961 ggagaaaaac cctatcaatg cagtgattgt gggaaagcct tcaatatgaa gacacaactc
     1021 attgtacatc agggagttca cacaggaaat aatccttatc aatgcggtga atgtgggaaa
     1081 gcctttggta ggaaggaaca gctcactgca catctgagag ctcatgcagg agagaagccc
     1141 tatggatgca gtgaatgtgg gaaggctttc agcagcaagt cataccttgt tatacatagg
     1201 agaacacaca ccggagagag accctatgaa tgtagtttgt gtgagagagc cttttgtgga
     1261 aaatcacagc tgattataca tcagagaact cattcaactg agaagcccta tgaatgcaat
     1321 gaatgtgaaa aagcctaccc taggaaggca tcacttcaga tacaccagaa aactcattcg
     1381 ggagagaaac cttttaaatg cagtgaatgt ggaaaagcct tcactcagaa gtcatctctc
     1441 agtgaacatc agagagttca caccggagag aaaccatgga aatgctctga atgtgggaaa
     1501 tccttctgtt ggaattcagg gcttcgtata catcggaaga ctcataaata gtaatgaact
     1561 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     1621 tgaatctgcc aagggtcgct tgc
//
