LOCUS       sequence-282083-        1478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1782
FEATURES             Location/Qualifiers
     source          1..1478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccagtac aattactgga agtcaggatt gtattgtgaa tcatcgaggg
       61 gaagtggatg gggagcctga actagatatt tccccttgtc aacagtgggg agaagcatct
      121 tctcctattt ccagaaacag ggacagtgtg atgactcttc aaagtggttg tttcgaaaac
      181 attgaaagtg aaacatattt gcctttgaaa gtctcaagcc aaatagacac acaagactct
      241 tcagtgaagt tctgtaagaa tgagcctcag gatcatcagg aaagcagacg tctctttgta
      301 atggaagaaa gcactgagag aaaagtgata aagggggaaa gttgttcaga gaaccttcaa
      361 gttaaactgg tgtctgatgg acaagaactg gcctcgccat tgttaaatgg tgaggcaact
      421 tgccagaatg gccagttaaa agaatctttg gatcccattg actgtaactg caaagacatt
      481 catggatgga aatcacaggt ggtcagttgt agtcagcaga gagctcatac agaggagaaa
      541 ccctgtgacc ataataactg tgggaaaata cttaacacca gcccagatgg tcatccatat
      601 gagaaaatcc acactgcaga gaaacaatac gaatgtagtc agtgtggtaa gaacttcagt
      661 caaagctcag agctactact tcatcagaga gaccacacag aagaaaaacc ctacaaatgt
      721 gagcaatgtg ggaagggctt cacaaggagc tcgagtctgc ttatccatca ggcagtccac
      781 acagatgaga agccttataa gtgtgacaag tgtgggaagg gcttcaccag gagctcaagt
      841 ctgctcatcc atcatgccgt ccatacaggc gaaaaacctt ataaatgtga caagtgtggg
      901 aagggcttta gtcagagctc caaactgcac atccaccagc gagtccacac tggagagaag
      961 ccctatgagt gtgaggagtg tggtatgagc ttcagtcagc gctcaaacct gcacatccac
     1021 cagcgagtac acacaggaga gaggccctac aagtgtggtg agtgtgggaa gggcttcagt
     1081 cagagctcga accttcacat tcaccggtgc atccacacag gagagaagcc ttaccaatgc
     1141 tatgagtgtg ggaagggttt cagccagagc tcggatcttc gcatccatct cagagtccac
     1201 actggagaga agccctatca ctgtggcaag tgtgggaagg gatttagcca gagttccaaa
     1261 ctcctcatcc accagagagt acatactgga gagaagccct atgagtgcag caagtgtggg
     1321 aagggcttca gccagagctc caaccttcac atccaccagc gggttcacaa gaaagatcct
     1381 cgctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1441 tgtggaaagg acgattggat ctcgacggac acgactgt
//
