LOCUS       sequence-282086-        3122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1796
FEATURES             Location/Qualifiers
     source          1..3122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatagcag ccaacagaaa acatttcgag gacatgatgc ccctgtttta
       61 agtctttcct ttgatcctaa ggacatcttt ctggcatcag ctagttgtga tggatctgtc
      121 agagtgtggc aaatttcaga tcagacatgt gctattagtt ggccactgct acaaaaatgc
      181 aacgatgtga taaatgcaaa atcaatctgc agacttgctt ggcagccaaa aagtgggaag
      241 ttactggcaa ttcctgtgga aaaatctgtt aagctatata gaagagaatc ttggagtcat
      301 caatttgatc tttcagataa tttcatctct cagaccctca atatagtaac ctggtctccc
      361 tgtgggcaat atttagctgc aggtagtatt aatggtctaa tcatagtttg gaatgtggaa
      421 accaaagact gcatggaaag ggtgaaacat gagaaaggtt atgcaatttg tggtctggca
      481 tggcatccta cttgtggtcg aatatcgtat actgatgcgg aaggaaatct agggcttcta
      541 gagaatgttt gtgaccccag tggaaagaca tcaagcagta aggtatctag cagagtggaa
      601 aaggattata atgatctttt tgatggagat gatatgagta atgctggtga ttttctaaat
      661 gacaatgcag ttgagatccc ttctttttca aaagggatta taaatgatga tgaggatgat
      721 gaagacctca tgatggcttc aggtcgtcct agacagcgaa gtcacatcct agaagatgat
      781 gaaaactcag ttgatatttc aatgctaaaa actggttcta gtcttctcaa agaggaggag
      841 gaagatggtc aagaaggcag cattcacaat ctaccacttg taacatccca aaggccattt
      901 tatgatggac ccatgccaac tccccggcaa aagccatttc agtcaggttc tacaccgttg
      961 catctcactc acagattcat ggtgtggaac tctattggaa ttattcgctg ctataatgat
     1021 gagcaagaca atgccataga tgtggagttc catgatacct ccatacacca tgcaacacac
     1081 ttatcaaaca ctttgaatta tacaatagca gatctttccc acgaagctat tttgttggca
     1141 tgtgaaagca ctgatgaact agcaagcaag cttcactgcc tgcactttag ttcttgggat
     1201 tcaagcaaag agtggataat agacttgcct cagaatgagg atattgaagc catatgtctc
     1261 ggtcaaggat gggctgctgc cgctactagt gccctgcttc ttcgattgtt tactattgga
     1321 ggggttcaaa aagaggtatt cagccttgct ggacctgtgg tgtcaatggc aggacatgga
     1381 gaacagcttt tcattgttta tcacagaggt acaggatttg atggggatca gtgccttgga
     1441 gttcaactgc tagagctggg gaaaaagaaa aaacaaattt tgcatggtga ccctcttcct
     1501 cttacaagga aatcctacct tgcatggatt gggttttcag ctgaaggtac cccttgttac
     1561 gtggattcag aaggaattgt tcgaatgctt aacagaggac ttggtaatac gtggactcct
     1621 atatgtaata caagagagca ctgcaaagga aaatctgatc actactgggt ggttggtatc
     1681 catgaaaatc cccagcaact aaggtgcatt ccttgtaaag gttctcggtt tcccccaacc
     1741 cttccacgcc ctgctgttgc tatattatcc tttaagcttc cttactgtca gattgcaaca
     1801 gagaaaggac aaatggagga gcaattttgg cgttcagtta tatttcacaa ccaccttgat
     1861 tatttagcta aaaatggtta tgaatatgaa gagagcacta aaaatcaagc aacaaaagag
     1921 caacaggaac ttttaatgaa aatgcttgcg ctttcttgta aactggagcg agaattccgt
     1981 tgtgtggaac ttgctgatct aatgactcaa aatgctgtga atttagccat taaatatgct
     2041 tctcgctctc ggaaattaat actggctcaa aaactaagtg aactggctgt agagaaggca
     2101 gccgaattga cagcaaccca ggtggaagag gaagaagaag aagaagattt cagaaaaaag
     2161 ctgaatgctg gttacagcaa tactgctaca gagtggagcc aaccaaggtt cagaaatcaa
     2221 gttgaagaag atgctgagga cagtggagaa gctgatgatg aagaaaaacc agaaatacat
     2281 aagcctggac agaactcgtt ttccaaaagt acaaattcct ctgatgtttc agctaagtca
     2341 ggtgcagtta cctttagcag ccaaggacga gtaaatccct ttaaggtatc agccagttcc
     2401 aaagaaccag ccatgtcaat gaattcagca cgttcaacta atattttaga caatatgggc
     2461 aaatcatcca agaaatccac tgcacttagt cgaactacaa ataatgaaaa gtctcccatt
     2521 ataaagcctc tgattccaaa gccgaagcct aagcaggcat ctgcagcatc ctatttccag
     2581 aaaagaaatt ctcaaactaa taaaactgag gaagtgaaag aagaaaatct taaaaatgta
     2641 ttatctgaaa ccccagctat atgtcctcct caaaacactg aaaaccaaag gccaaagacc
     2701 gggttccaga tgtggttaga agaaaataga agtaatattt tgtctgacaa tcctgacttt
     2761 tcagatgaag cagacataat aaaagaagga atgattcgat ttagagtatt gtcaactgaa
     2821 gaaagaaagg tgtgggctaa caaagccaaa ggagaaacgg caagtgaagg aactgaagca
     2881 aagaagcgaa aacgtgtggt tgatgaaagt gatgaaacag aaaaccagga agaaaaagca
     2941 aaagagaacc tgaatttgtc taaaaagcag aaacctttag atttttctac aaatcagaaa
     3001 ctatcagctt ttgcatttaa gcaggagtag taatgaacta gtccgtaact tgaaagtatt
     3061 tcgatttctt ggctttatat atcttgtgga aaggacgacg aagggccgaa tgtatgtgag
     3121 ag
//
