LOCUS       sequence-282101-        1814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1814
FEATURES             Location/Qualifiers
     source          1..1814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagaacct gaagtctgga gtgtatcctc tcaaggaagc aagtggatgc
       61 cctggggctg acaggaatct tctggtgtac tctttttatg aaaaggggcc attgacattt
      121 agggatgtgg ccatagaatt ttctctggag gagtggcaat gcctggacac tgctcagcag
      181 gatttgtata gaaaagtgat gttagagaac tacagaaacc tggtcttctt ggcaggtatt
      241 gctgtttcta agccagacct gatcacctgt ctagagcaag gaaaagagcc ctggaatatg
      301 aagagacatg cgatggtaga tcaaccccca gttacatatt ctcattttgc ccaagacctt
      361 tggccagagc agggcataaa agattctttc caagaagtca tattgagaag atatggaaaa
      421 tgtggacatg aagatttaca gttaagaaca ggctgtaaaa gtgtggatga gtgtaatctg
      481 cacaaagaat gttatgatga actaaaccag tgtttgacaa ctacccagag tgaaatattt
      541 caatatgata aatatgtgaa tgtcttttat aaattttcaa atccaaatat acaaaagata
      601 agacatactg gaaagaagcc tttcaaatgt aaaaaatgtg acaaatcgtt ttgcatgctt
      661 ttacacctaa ctcaacataa aagaattcat attagggaaa attcttacca atgtgaagaa
      721 tgtggtaaag tctttaactg gttctcaacc cttactagac acagaagaat tcatactgga
      781 gagaaaccct acaaatgtga acaatgtggc aaagctttta agcagtcctc aacccttact
      841 acacataaga taattcatac tggagagaaa ccctacagat gtgaagaatg tggcaaaacc
      901 tttaaccggt cctcacacct tactacacat aaaagaattc atactggaga gaaaccctac
      961 agatgtgaag aatgtggcag agcttttaac cggtcctcac accttactac acataagatt
     1021 attcatactg gagagaaacc ctacaaatgt gaagaatgtg gcaaagcttt taaccaatcc
     1081 tcaaccctta ctacacataa gataattcat gctggagaga aaccttacaa atgtgaagaa
     1141 tgtggcaaag ctttttaccg attctcatac cttactaaac ataagataat tcatactgga
     1201 gagaaattct acaaatgtga agaatgtggc aaaggcttta attggtcctc gacccttact
     1261 aaacataaaa gaattcatac tggagagaaa ccctacaaat gtgaacaatg tggcaaagct
     1321 tttaatgagt cctcaaacct tactgcacat aagataattc atactggaga gaaaccctac
     1381 aaatgtgaag aatgtggcaa agcctttaac cggtccccaa aacttactgc acataaggta
     1441 attcattctg gagagaaacc ctacaaatgt gaagaatgtg gcaaagcttt taaccaattc
     1501 tcaaacctta ctaaacataa gataactcat attggagata catcttacaa atacctagaa
     1561 tgtgataaag cctttagcca gtcttcaact cttactaaac ataaggtaat tcatactgga
     1621 gagaaaccct acaactgtga agaatacggc aaagctttca accagtcctc aaaccttatt
     1681 gaacaaagta attcatactg gagagaaacc ctacaaatgt agtaatgaac tagtccgtaa
     1741 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tcgatgttgc
     1801 gtccgggctc atag
//
