LOCUS       sequence-282107-        2225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1835
FEATURES             Location/Qualifiers
     source          1..2225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaatacc aaatccccct acctccaaat gtatcactta ctggaaaaga
       61 aaagtgaaat ctgaatacat gcgacttcga caacttaaac ggcttcaggc aaatatgggt
      121 gcaaaggctt tgtatgtggc aaattttgca aaggttcaag aaaaaaccca gatcctcaat
      181 gaagaatgga agaagcttcg tgtccaacct gttcagtcaa tgaagcctgt gagtggacac
      241 ccttttctca aaaaggtaga agatgagacg gttttgtgca atattcccta catgggagat
      301 gaagtgaaag aagaagatga gacttttatt gaggagctga tcaataacta tgatgggaaa
      361 gtccatggtg aagaagagat gatccctgga tccgttctga ttagtgatgc tgtttttctg
      421 gagttggtcg atgccctgaa tcagtactca gatgaggagg aggaagggca caatgacacc
      481 tcagatggaa agcaggatga cagcaaagaa gatctgccag taacaagaaa gagaaagcga
      541 catgctattg aaggcaacaa aaagagttcc aagaaacagt tcccaaatga catgatcttc
      601 agtgcaattg cctcaatgtt ccctgagaat ggtgtcccag atgacatgaa ggagaggtat
      661 cgagaactaa cagagatgtc agaccccaat gcacttcccc ctcagtgcac acccaacatc
      721 gatggcccca atgccaagtc tgtgcagcgg gagcaatctc tgcactcctt ccacacactt
      781 ttttgccggc gctgctttaa atacgactgc ttccttcacc cttttcatgc cacccctaat
      841 gtatataaac gcaagaataa agaaatcaag attgaaccag aaccatgtgg cacagactgc
      901 ttccttttgc tggaaggagc aaaggagtat gccatgctcc acaacccccg ctccaagtgc
      961 tctggtcgtc gccggagaag gcaccacata gtcagtgctt cctgctccaa tgcctcagcc
     1021 tctgctgtgg ctgagactaa agaaggagac agtgacaggg acacaggcaa tgactgggcc
     1081 tccagttctt cagaggctaa ctctcgctgt cagactccca caaaacagaa ggctagtcca
     1141 gccccacctc aactctgcgt agtggaagca ccctcggagc ctgtggaatg gactggggct
     1201 gaagaatctc tttttcgagt cttccatggc acctacttca acaacttctg ttcaatagcc
     1261 aggcttctgg ggaccaagac gtgcaagcag gtctttcagt ttgcagtcaa agaatcactt
     1321 atcctgaagc tgccaacaga tgagctcatg aacccctcac agaagaagaa aagaaagcac
     1381 agattgtggg ctgcacactg caggaagatt cagctgaaga aagataactc ttccacacaa
     1441 gtgtacaact accaaccctg cgaccaccca gaccgcccct gtgacagcac ctgcccctgc
     1501 atcatgactc agaatttctg tgagaagttc tgccagtgca acccagactg tcagaatcgt
     1561 ttccctggct gtcgctgtaa gacccagtgc aataccaagc aatgtccttg ctatctggca
     1621 gtgcgagaat gtgaccctga cctgtgtctc acctgtgggg cctcagagca ctgggactgc
     1681 aaggtggttt cctgtaaaaa ctgcagcatc cagcgtggac ttaagaagca cctgctgctg
     1741 gccccctctg atgtggccgg atggggcacc ttcataaagg agtctgtgca gaagaacgaa
     1801 ttcatttctg aatactgtgg tgagctcatc tctcaggatg aggctgatcg acgcggaaag
     1861 gtctatgaca aatacatgtc cagcttcctc ttcaacctca ataatgattt tgtagtggat
     1921 gctactcgga aaggaaacaa aattcgattt gcaaatcatt cagtgaatcc caactgttat
     1981 gccaaagtgg tcatggtgaa tggagaccat cggattggga tctttgccaa gagggcaatt
     2041 caagctggcg aagagctctt ctttgattac aggtacagcc aagctgatgc tctcaagtac
     2101 gtggggatcg agagggagac cgacgtcctt tagtaatgaa ctagtccgta acttgaaagt
     2161 atttcgattt cttggcttta tatatcttgt ggaaaggacg ataacgtcga gatgtgccgt
     2221 ttctt
//
