LOCUS       sequence-282131-        3362 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2006
FEATURES             Location/Qualifiers
     source          1..3362
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccacggt cggagagtgg tcctgtgtgc gctgcacctt cctgaacccg
       61 gccggccagc gccagtgctc catctgcgag gctccccggc acaagcccga cctcaaccac
      121 atcctgcggc tcagcgtgga ggagcagaaa tggccctgcg cccgctgcac cttccgcaac
      181 ttcctgggca aggaggcctg cgaggtgtgc ggcttcaccc cggagcctgc gcctggggct
      241 gccttcctgc cagtcctcaa cggggtcctc cccaagccac ccgccatcct gggggagccc
      301 aagggcagct gccaggagga agcaggtcca gtgaggactg cggggctggt ggccacggag
      361 cccgccaggg ggcagtgcga ggacaaggac gaggaggaga aggaggagca ggaggaggag
      421 gagggagcgg cggagcccag agggggctgg gcgtgtccgc gttgcacgct gcacaacacg
      481 cccgtggcca gctcctgctc cgtctgcggg ggcccacgca ggctctcgct gccacggatc
      541 cctcctgagg ccctggtggt cccggaagtg gtggccccgg ccggcttcca cgtcgtgcct
      601 gccgcgcctc cacctggcct ccccggggaa ggtgccgagg ccaacccccc agccaccagc
      661 cagggcccag ctgccgaacc agagccgccc agggtcccgc ccttcagccc cttctcgtcc
      721 accctgcaga acaaccccgt gccgcgcagc cgacgcgagg ttccccccca gctgcagcca
      781 ccggtgcctg aggctgccca gccgtcaccc tctgccggct gcaggggagc cccccagggc
      841 tcgggctggg ctggggcctc ccgcctagca gagttgctgt ctggcaagcg gctgagtgtg
      901 ctggaggaag aggccacgga gggtggcacc agccgcgtag aggccggcag ctccacctcg
      961 ggcagtgaca tcattgacct ggccggagac accgtgcgtt acacgcccgc cagcccctcc
     1021 agccccgact tcaccacctg gtcatgtgcc aagtgcacgc tcagaaaccc cacagtggcc
     1081 cccaggtgct cggcctgcgg ctgctccaaa ctgcacggct tccaggagca tggcgagccc
     1141 cccacccact gccccgactg tggggccgac aagcccagcc cctgcggcag aagctgcgga
     1201 cgggtgtcct cggcccagaa ggccgcccgc gtcctgcccg agcgcccggg ccagtgggcc
     1261 tgccctgcct gtaccctgct caacgcactg cgggccaagc actgcgccgc ctgccacacg
     1321 cctcagctcc tggtggccca gcggcggggg gccgcgcccc tgaggcgcag ggagagcatg
     1381 cacgtggagc agcggcggca gacagacgag ggcgaggcca aggcactctg ggagaacatc
     1441 gtggccttct gccgggagaa caatgtgagc ttcgtggatg acagcttccc tcccgggccc
     1501 gagtctgtcg gcttccccgc gggtgacagc gtgcagcagc gtgtgaggca gtggctgcga
     1561 ccccaggaga tcaactgctc cgtcttcagg gaccacaggg ccacgtggtc tgtgttccac
     1621 acactgcggc cctcagacat cctgcagggg ctgctgggga actgctggtt cctgagcgcc
     1681 ctggcggtgc tggcggagcg gccggacctg gtggagcggg tgatggtcac gcgcagcctg
     1741 tgtgcagagg gcgcctacca ggtgcggctg tgcaaggacg gcacgtggac cacggtgctg
     1801 gtggacgaca tgctgccctg tgatgaggcc ggctgcctcc tcttctcaca ggcgcagcgg
     1861 aagcagctgt gggtggccct catcgagaag gcgctggcca agctgcacgg ctcctacttt
     1921 gcgctccagg cgggccgcgc catcgaaggc ctggccacgc tcaccggcgc cccctgtgag
     1981 agcctggcgc tgcagctcag ctccactaac ccccgcgagg agcccgttga cactgacctc
     2041 atctgggcca aaatgctgag ttctaaggag gctgggttcc tcatgggtgc ctcctgtggc
     2101 gggggcaaca tgaaggtgga cgattcggcc tacgagagcc tgggcctgcg cccccggcat
     2161 gcctactcca tcctggatgt ccgagatgtc cagggcacca ggcttctgcg gctccgaaac
     2221 ccgtggggcc gtttctcctg gaacggcagc tggtccgacg agtggccaca ctggccgggg
     2281 cacctgcgtg gcgagctcat gccgcacggc agcagtgagg gtgtcttctg gatggagtac
     2341 ggcgactttg tcaggtactt cgactccgtg gacatctgta aggtgcactc ggactggcag
     2401 gaggcgcggg tgcagggctg ctttcccagc tcggccagcg cgcccgtggg ggtaacagcg
     2461 ctcacggtgc tggagcgggc ctcgctggag ttcgcgctct tccaggaggg cagcaggcgc
     2521 tcggacgccg tggacagcca cctgctggac ctgtgcatcc tggtgttccg ggccacgttc
     2581 ggcagcggcg gccacctcag cctgggccgc ctcctggccc acagtaagcg cgcggtcaag
     2641 aagttcgtca gctgcgacgt catgctggag cctggcgagt acgctgtggt gtgctgcgcc
     2701 ttcaaccact gggggccgcc cctgccgggc acccctgccc cccaggcctc cagcccctcg
     2761 gcaggggtcc cgagagcctc cccagagccg ccgggccacg tgctggctgt gtacagctcg
     2821 aggctggtca tggtggagcc cgtggaagcc cagccgacca cgctggccga cgccatcatc
     2881 ctgctcaccg agagccgcgg agagcggcac gagggccgtg agggcatgac ctgctactac
     2941 ctgacacacg gttgggcggg gctcatcgtg gtggtggaga accgacaccc caaggcctac
     3001 ctgcacgtgc agtgtgactg caccgacagc ttcaacgtgg tgtccacacg cggcagcctg
     3061 cgtacccagg atagcgtgcc acccctgcac aggcaggtcc tggtgatctt gtcccagcta
     3121 gagggcaacg ccggcttctc tatcacccac cgcctggcac atcgcaaggc agcccaggcc
     3181 ttcctcagtg actggacagc ctccaagggg acccacagcc ccccactcac gccagaggtc
     3241 gccggtctgc atgggccccg accgctgtag taatgaacta gtccgtaact tgaaagtatt
     3301 tcgatttctt ggctttatat atcttgtgga aaggacgagg acgctattga ggtaacccac
     3361 gt
//
