LOCUS       sequence-282137-        3746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2066
FEATURES             Location/Qualifiers
     source          1..3746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggccac cggggtgctg ccgttcgtgc gtggcgtgga cctcagcggc
       61 aacgacttca agggcggcta cttccctgag aatgtcaagg ccatgaccag cctgcggtgg
      121 ctgaagctga accgcactgg cctctgctac ctgcccgagg agctggccgc cctgcagaag
      181 ctggaacact tgtctgtgag ccacaacaac ctgaccacgc ttcatgggga gctgtccagc
      241 ctgccatcgc tgcgcgccat cgtggcccga gccaacagtc tgaagaattc cggagtcccc
      301 gatgacatct tcaagctaga tgatctctca gtcctggact tgagccacaa ccagctgaca
      361 gagtgcccgc gggagctgga gaacgccaag aacatgctgg tgctgaacct cagccacaac
      421 aggcagctcc cagcgatgac ggccctgcag accctgcacc tgcggagcac ccagcgcacc
      481 cagagcaacc tgcccaccag cctggagggt ctgagcaacc tcgcagacgt ggatctgtcc
      541 tgcaatgacc tgacacgggt gcccgagtgt ctgtacaccc tccccagcct gcgccgcctc
      601 aacctcagca gcaaccagat cacggagctg tccctgtgca tagaccagtg ggtgcacgtg
      661 gaaactctga acctgtcccg aaatcagctc acctcactgc cctcagccat ttgcaagctg
      721 agcaagctga agaagctgta cctgaattcc aacaagctgg actttgacgg gctgccctca
      781 ggcattggca agctcaccaa cctggaagag ttcatggctg ccaacaacaa cctggagctg
      841 gtccctgaaa gtctctgcag gtgcccaaag ctgaggaaac ttgtcctgaa caagaaccac
      901 ctggtgaccc tcccagaagc catccatttc ctgacggaga tcgaggtcct ggatgtgcgg
      961 gagaacccca acctggtcat gccgcccaag cccgcagacc gtgccgctga gtggtacaac
     1021 atcgacttct cgctgcagaa ccagctgcgg ctagcgggtg cctctcctgc taccgtggct
     1081 gcagctgcag ctgggagtgg gcccaaggac cctatggctc gcaagatgcg actgcggagg
     1141 cgcaaggatt cagcccagga tgaccaggcc aagcaggtgc tgaagggcat gtcagatgtt
     1201 gcccaggaga agaacaaaaa gcaggaggag agcgcagatg cccgggcccc cagcgggaag
     1261 gtgcggcgtt gggaccaggg cctggagaag ccccgccttg actactccga gttcttcacg
     1321 gaggacgtgg gccagctgcc cggactgacc atctggcaga tagagaactt cgtgcctgtg
     1381 ctggtggagg aagccttcca cggcaagttc tacgaggctg actgctacat tgtgctcaag
     1441 acctttctgg atgacagcgg ctccctcaac tgggagatct actactggat tggcggggag
     1501 gccacactcg acaagaaagc ttgctctgcc atccacgctg tcaacttgcg caactacctg
     1561 ggtgctgagt gccgcactgt ccgggaggag atgggcgatg agagcgagga gttcctgcag
     1621 gtgtttgaca acgacatctc ctacattgag ggtggaacag ccagtggctt ctacactgtg
     1681 gaagacacac actatgtcac caggatgtat cgtgtgtatg ggaaaaagaa catcaagttg
     1741 gagcctgtgc ccctcaaggg gacctctctg gacccaaggt ttgttttcct gctggaccga
     1801 gggctagaca tctacgtatg gcggggggcc caggccacac tgagcagcac caccaaggcc
     1861 aggctctttg cagagaaaat taacaagaat gagcggaaag ggaaggctga gatcacactg
     1921 ctggtgcagg gccaggagct cccagagttc tgggaggcac tgggtgggga gccctctgag
     1981 atcaagaagc acgtgcctga agacttctgg ccgccgcagc ccaagctgta caaggtgggc
     2041 ctgggcttgg gctacctgga gctgccacag atcaactaca agctctccgt ggaacataag
     2101 cagcgtccca aggtggagct gatgccaaga atgcggctgc tgcagagtct gctggacacg
     2161 cgctgcgtgt acattctgga ctgttggtcc gacgtgttca tctggctcgg ccgcaagtcc
     2221 ccgcgcctgg tgcgcgctgc cgccctcaag ctgggtcagg agctgtgcgg gatgctgcac
     2281 cggccacgcc atgccacggt cagccgcagc ctcgagggca ccgaggcgca ggtgttcaag
     2341 gccaagttca agaattggga cgatgtgttg acggtggact acacacgcaa tgcggaggcc
     2401 gtgctgcaga gcccgggtct ctccgggaag gtgaaacgcg acgccgagaa gaaagaccag
     2461 atgaaggctg acctcactgc gcttttcctg ccgcggcagc cgcccatgtc gctggccgag
     2521 gcggagcagc tgatggagga gtggaacgaa gacctagacg gcatggaggg tttcgtgctg
     2581 gagggcaaga agtttgcgcg gctgccggaa gaggagtttg gccacttcta cacgcaggac
     2641 tgctacgtct tcctctgcag gtactgggtg cctgtggagt acgaggagga ggaaaagaag
     2701 gaagacaagg aggagaaggc cgagggcaaa gaaggcgagg aagcaaccgc tgaggcagag
     2761 gagaagcagc cagaggagga cttccagtgc atcgtgtact tctggcaggg ccgtgaagcc
     2821 tccaatatgg gctggctcac cttcaccttc agcctgcaaa agaagttcga gagcctcttc
     2881 cctgggaagc tggaggtggt acgcatgacg cagcagcagg agaaccccaa gttcctgtcc
     2941 catttcaaga ggaagttcat catccaccgg ggcaagagga aggcggtcca gggcgcccaa
     3001 cagcccagcc tctaccagat ccgcaccaac ggcagcgccc tctgcacccg gtgcatccag
     3061 atcaacaccg actccagcct cctcaactcc gagttctgct tcatcctcaa ggttcccttt
     3121 gagagtgagg acaaccaggg catcgtgtat gcctgggtgg gccgggcatc agaccctgac
     3181 gaagccaagt tggcagaaga catcctgaac accatgtttg acacctccta cagcaagcag
     3241 gttatcaacg aaggtgagga gcctgagaac ttcttctggg tgggcattgg ggcacagaag
     3301 ccctatgatg acgatgccga gtacatgaaa cacacacgtc tcttccggtg ctccaacgag
     3361 aagggctact ttgcagtgac tgagaaatgc tccgactttt gccaagatga cctggcagat
     3421 gatgacatca tgttgctaga caatggccaa gaggtctaca tgtgggtggg gacccagact
     3481 agccaggtgg agatcaagct gagcctgaag gcctgccagg tatatatcca gcacatgcgg
     3541 tccaaggaac atgagcggcc gcgccggctg cgcctggtcc gcaagggcaa tgagcagcac
     3601 gcctttaccc gctgcttcca cgcctggagc gccttctgca aggccctggc ctagtaatga
     3661 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     3721 gaggtaacgg atcttacagt caagat
//
