LOCUS       sequence-282140-        3878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2067
FEATURES             Location/Qualifiers
     source          1..3878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacctccg aggcctccgc ccagtgcctg gcggctactt ccctgagaat
       61 gtcaaggcca tgaccagcct gcggtggctg aagctgaacc gcactggcct ctgctacctg
      121 cccgaggagc tggccgccct gcagaagctg gaacacttgt ctgtgagcca caacaacctg
      181 accacgcttc atggggagct gtccagcctg ccatcgctgc gcgccatcgt ggcccgagcc
      241 aacagtctga agaattccgg agtccccgat gacatcttca agctagatga tctctcagtc
      301 ctggacttga gccacaacca gctgacagag tgcccgcggg agctggagaa cgccaagaac
      361 atgctggtgc tgaacctcag ccacaacagc atcgacacca tccccaacca gctcttcatc
      421 aacctcactg acctactata cctggacctc agcgagaacc gcctggagag cctgcccccg
      481 cagatgcgcc gcctggtgca cctgcagacg ctcgtgctca atggaaaccc cctgctgcat
      541 gcacagctcc ggcagctccc agcgatgacg gccctgcaga ccctgcacct gcggagcacc
      601 cagcgcaccc agagcaacct gcccaccagc ctggagggtc tgagcaacct cgcagacgtg
      661 gatctgtcct gcaatgacct gacacgggtg cccgagtgtc tgtacaccct ccccagcctg
      721 cgccgcctca acctcagcag caaccagatc acggagctgt ccctgtgcat agaccagtgg
      781 gtgcacgtgg aaactctgaa cctgtcccga aatcagctca cctcactgcc ctcagccatt
      841 tgcaagctga gcaagctgaa gaagctgtac ctgaattcca acaagctgga ctttgacggg
      901 ctgccctcag gcattggcaa gctcaccaac ctggaagagt tcatggctgc caacaacaac
      961 ctggagctgg tccctgaaag tctctgcagg tgcccaaagc tgaggaaact tgtcctgaac
     1021 aagaaccacc tggtgaccct cccagaagcc atccatttcc tgacggagat cgaggtcctg
     1081 gatgtgcggg agaaccccaa cctggtcatg ccgcccaagc ccgcagaccg tgccgctgag
     1141 tggtacaaca tcgacttctc gctgcagaac cagctgcggc tagcgggtgc ctctcctgct
     1201 accgtggctg cagctgcagc tgcagggagt gggcccaagg accctatggc tcgcaagatg
     1261 cgactgcgga ggcgcaagga ttcagcccag gatgaccagg ccaagcaggt gctgaagggc
     1321 atgtcagatg ttgcccagga gaagaacaaa aagcaggagg agagcgcaga tgcccgggcc
     1381 cccagcggga aggtgcggcg ttgggaccag ggcctggaga agccccgcct tgactactcc
     1441 gagttcttca cggaggacgt gggccagctg cccggactga ccatctggca gatagagaac
     1501 ttcgtgcctg tgctggtgga ggaagccttc cacggcaagt tctacgaggc tgactgctac
     1561 attgtgctca agacctttct ggatgacagc ggctccctca actgggagat ctactactgg
     1621 attggcgggg aggccacact cgacaagaaa gcttgctctg ccatccacgc tgtcaacttg
     1681 cgcaactacc tgggtgctga gtgccgcact gtccgggagg agatgggcga tgagagcgag
     1741 gagttcctgc aggtgtttga caacgacatc tcctacattg agggtggaac agccagtggc
     1801 ttctacactg tggaagacac acactatgtc accaggatgt atcgtgtgta tgggaaaaag
     1861 aacatcaagt tggagcctgt gcccctcaag gggacctctc tggacccaag gtttgttttc
     1921 ctgctggacc gagggctaga catctacgta tggcgggggg cccaggccac actgagcagc
     1981 accaccaagg ccaggctctt tgcagagaaa attaacaaga atgagcggaa agggaaggct
     2041 gagatcacac tgctggtgca gggccaggag ctcccagagt tctgggaggc actgggtggg
     2101 gagccctctg agatcaagaa gcacgtgcct gaagacttct ggccgccgca gcccaagctg
     2161 tacaaggtgg gcctgggctt gggctacctg gagctgccac agatcaacta caagctctcc
     2221 gtggaacata agcagcgtcc caaggtggag ctgatgccaa gaatgcggct gctgcagagt
     2281 ctgctggaca cgcgctgcgt gtacattctg gactgttggt ccgacgtgtt catctggctc
     2341 ggccgcaagt ccccgcgcct ggtgcgcgct gccgccctca agctgggtca ggagctgtgc
     2401 gggatgctgc accggccacg ccatgccacg gtcagccgca gcctcgaggg caccgaggcg
     2461 caggtgttca aggccaagtt caagaattgg gacgatgtgt tgacggtgga ctacacacgc
     2521 aatgcggagg ccgtgctgca gagcccgggt ctctccggga aggtgaaacg cgacgccgag
     2581 aagaaagacc agatgaaggc tgacctcact gcgcttttcc tgccgcggca gccgcccatg
     2641 tcgctggccg aggcggagca gctgatggag gagtggaacg aagacctaga cggcatggag
     2701 ggtttcgtgc tggagggcaa gaagtttgcg cggctgccgg aagaggagtt tggccacttc
     2761 tacacgcagg actgctacgt cttcctctgc aggtactggg tgcctgtgga gtacgaggag
     2821 gaggaaaaga aggaagacaa ggaggagaag gccgagggca aagaaggcga ggaagcaacc
     2881 gctgaggcag aggagaagca gccagaggag gacttccagt gcatcgtgta cttctggcag
     2941 ggccgtgaag cctccaatat gggctggctc accttcacct tcagcctgca aaagaagttc
     3001 gagagcctct tccctgggaa gctggaggtg gtacgcatga cgcagcagca ggagaacccc
     3061 aagttcctgt cccatttcaa gaggaagttc atcatccacc ggggcaagag gaaggcggtc
     3121 cagggcgccc aacagcccag cctctaccag atccgcacca acggcagcgc cctctgcacc
     3181 cggtgcatcc agatcaacac cgactccagc ctcctcaact ccgagttctg cttcatcctc
     3241 aaggttccct ttgagagtga ggacaaccag ggcatcgtgt atgcctgggt gggccgggca
     3301 tcagaccctg acgaagccaa gttggcagaa gacatcctga acaccatgtt tgacacctcc
     3361 tacagcaagc aggttatcaa cgaaggtgag gagcctgaga acttcttctg ggtgggcatt
     3421 ggggcacaga agccctatga tgacgatgcc gagtacatga aacacacacg tctcttccgg
     3481 tgctccaacg agaagggcta ctttgcagtg actgagaaat gctccgactt ttgccaagat
     3541 gacctggcag atgatgacat catgttgcta gacaatggcc aagaggtcta catgtgggtg
     3601 gggacccaga ctagccaggt ggagatcaag ctgagcctga aggcctgcca ggtatatatc
     3661 cagcacatgc ggtccaagga acatgagcgg ccgcgccggc tgcgcctggt ccgcaagggc
     3721 aatgagcagc acgcctttac ccgctgcttc cacgcctgga gcgccttctg caaggccctg
     3781 gcctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     3841 tgtggaaagg acgaaaggtc tgtgatgcga gtcctaat
//
