LOCUS       sequence-282143-        3911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2068
FEATURES             Location/Qualifiers
     source          1..3911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggccac cggggtgctg ccgttcgtgc gtggcgtgga cctcagcggc
       61 aacgacttca agggcggcta cttccctgag aatgtcaagg ccatgaccag cctgcggtgg
      121 ctgaagctga accgcactgg cctctgctac ctgcccgagg agctggccgc cctgcagaag
      181 ctggaacact tgtctgtgag ccacaacaac ctgaccacgc ttcatgggga gctgtccagc
      241 ctgccatcgc tgcgcgccat cgtggcccga gccaacagtc tgaagaattc cggagtcccc
      301 gatgacatct tcaagctaga tgatctctca gtcctggact tgagccacaa ccagctgaca
      361 gagtgcccgc gggagctgga gaacgccaag aacatgctgg tgctgaacct cagccacaac
      421 agcatcgaca ccatccccaa ccagctcttc atcaacctca ctgacctact atacctggac
      481 ctcagcgaga accgcctgga gagcctgccc ccgcagatgc gccgcctggt gcacctgcag
      541 acgctcgtgc tcaatggaaa ccccctgctg catgcacagc tccggcagct cccagcgatg
      601 acggccctgc agaccctgca cctgcggagc acccagcgca cccagagcaa cctgcccacc
      661 agcctggagg gtctgagcaa cctcgcagac gtggatctgt cctgcaatga cctgacacgg
      721 gtgcccgagt gtctgtacac cctccccagc ctgcgccgcc tcaacctcag cagcaaccag
      781 atcacggagc tgtccctgtg catagaccag tgggtgcacg tggaaactct gaacctgtcc
      841 cgaaatcagc tcacctcact gccctcagcc atttgcaagc tgagcaagct gaagaagctg
      901 tacctgaatt ccaacaagct ggactttgac gggctgccct caggcattgg caagctcacc
      961 aacctggaag agttcatggc tgccaacaac aacctggagc tggtccctga aagtctctgc
     1021 aggtgcccaa agctgaggaa acttgtcctg aacaagaacc acctggtgac cctcccagaa
     1081 gccatccatt tcctgacgga gatcgaggtc ctggatgtgc gggagaaccc caacctggtc
     1141 atgccgccca agcccgcaga ccgtgccgct gagtggtaca acatcgactt ctcgctgcag
     1201 aaccagctgc ggctagcggg tgcctctcct gctaccgtgg ctgcagctgc agctgcaggg
     1261 agtgggccca aggaccctat ggctcgcaag atgcgactgc ggaggcgcaa ggattcagcc
     1321 caggatgacc aggccaagca ggtgctgaag ggcatgtcag atgttgccca ggagaagaac
     1381 aaaaagcagg aggagagcgc agatgcccgg gcccccagcg ggaaggtgcg gcgttgggac
     1441 cagggcctgg agaagccccg ccttgactac tccgagttct tcacggagga cgtgggccag
     1501 ctgcccggac tgaccatctg gcagatagag aacttcgtgc ctgtgctggt ggaggaagcc
     1561 ttccacggca agttctacga ggctgactgc tacattgtgc tcaagacctt tctggatgac
     1621 agcggctccc tcaactggga gatctactac tggattggcg gggaggccac actcgacaag
     1681 aaagcttgct ctgccatcca cgctgtcaac ttgcgcaact acctgggtgc tgagtgccgc
     1741 actgtccggg aggagatggg cgatgagagc gaggagttcc tgcaggtgtt tgacaacgac
     1801 atctcctaca ttgagggtgg aacagccagt ggcttctaca ctgtggaaga cacacactat
     1861 gtcaccagga tgtatcgtgt gtatgggaaa aagaacatca agttggagcc tgtgcccctc
     1921 aaggggacct ctctggaccc aaggtttgtt ttcctgctgg accgagggct agacatctac
     1981 gtatggcggg gggcccaggc cacactgagc agcaccacca aggccaggct ctttgcagag
     2041 aaaattaaca agaatgagcg gaaagggaag gctgagatca cactgctggt gcagggccag
     2101 gagctcccag agttctggga ggcactgggt ggggagccct ctgagatcaa gaagcacgtg
     2161 cctgaagact tctggccgcc gcagcccaag ctgtacaagg tgggcctggg cttgggctac
     2221 ctggagctgc cacagatcaa ctacaagctc tccgtggaac ataagcagcg tcccaaggtg
     2281 gagctgatgc caagaatgcg gctgctgcag agtctgctgg acacgcgctg cgtgtacatt
     2341 ctggactgtt ggtccgacgt gttcatctgg ctcggccgca agtccccgcg cctggtgcgc
     2401 gctgccgccc tcaagctggg tcaggagctg tgcgggatgc tgcaccggcc acgccatgcc
     2461 acggtcagcc gcagcctcga gggcaccgag gcgcaggtgt tcaaggccaa gttcaagaat
     2521 tgggacgatg tgttgacggt ggactacaca cgcaatgcgg aggccgtgct gcagagcccg
     2581 ggtctctccg ggaaggtgaa acgcgacgcc gagaagaaag accagatgaa ggctgacctc
     2641 actgcgcttt tcctgccgcg gcagccgccc atgtcgctgg ccgaggcgga gcagctgatg
     2701 gaggagtgga acgaagacct agacggcatg gagggtttcg tgctggaggg caagaagttt
     2761 gcgcggctgc cggaagagga gtttggccac ttctacacgc aggactgcta cgtcttcctc
     2821 tgcaggtact gggtgcctgt ggagtacgag gaggaggaaa agaaggaaga caaggaggag
     2881 aaggccgagg gcaaagaagg cgaggaagca accgctgagg cagaggagaa gcagccagag
     2941 gaggacttcc agtgcatcgt gtacttctgg cagggccgtg aagcctccaa tatgggctgg
     3001 ctcaccttca ccttcagcct gcaaaagaag ttcgagagcc tcttccctgg gaagctggag
     3061 gtggtacgca tgacgcagca gcaggagaac cccaagttcc tgtcccattt caagaggaag
     3121 ttcatcatcc accggggcaa gaggaaggcg gtccagggcg cccaacagcc cagcctctac
     3181 cagatccgca ccaacggcag cgccctctgc acccggtgca tccagatcaa caccgactcc
     3241 agcctcctca actccgagtt ctgcttcatc ctcaaggttc cctttgagag tgaggacaac
     3301 cagggcatcg tgtatgcctg ggtgggccgg gcatcagacc ctgacgaagc caagttggca
     3361 gaagacatcc tgaacaccat gtttgacacc tcctacagca agcaggttat caacgaaggt
     3421 gaggagcctg agaacttctt ctgggtgggc attggggcac agaagcccta tgatgacgat
     3481 gccgagtaca tgaaacacac acgtctcttc cggtgctcca acgagaaggg ctactttgca
     3541 gtgactgaga aatgctccga cttttgccaa gatgacctgg cagatgatga catcatgttg
     3601 ctagacaatg gccaagaggt ctacatgtgg gtggggaccc agactagcca ggtggagatc
     3661 aagctgagcc tgaaggcctg ccaggtatat atccagcaca tgcggtccaa ggaacatgag
     3721 cggccgcgcc ggctgcgcct ggtccgcaag ggcaatgagc agcacgcctt tacccgctgc
     3781 ttccacgcct ggagcgcctt ctgcaaggcc ctggcctagt aatgaactag tccgtaactt
     3841 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaata gtagcaagtg
     3901 acgtggaggc t
//
