LOCUS       sequence-282149-        1703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2093
FEATURES             Location/Qualifiers
     source          1..1703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccatgg ttcagtgaca ttcagggatg tggccataga cttctcacag
       61 gaagaatggg aattcctgga tcctgctcag agggacttat atagggatgt aatgtgggag
      121 aactacagca acttcatttc actaggacct tccatttcta aaccagatgt gattacctta
      181 ttggatgaag aaaggaagga acctgggatg gttgtgaggg aagggacaag aagatactgc
      241 cctgatttgg agtccagata caggaccaat actttatctc cagaaaagga catttatgaa
      301 atatattcat ttcagtggga tataatggaa agaattaaaa gctatagcct tcagggttcc
      361 atttttagga atgattggga atgcaaaagc aagattgagg gggaaaagga acaacaagag
      421 ggatattttg ggcaagtgaa aattacctct gaaaaaatga ccacttacaa aaggcacaat
      481 tttcttactg agtatcagat cgttcataat ggagaaaagg tgtatgagtg taaggagtgt
      541 aggaagacct ttattcgtcg ctcaacactt agtcaacacc tgagaattca tactggtgag
      601 aaaccttata agtgtaagga atgtgggcag gcctttagac agcgtgcaca tcttattcga
      661 catcacaaac ttcacaccgg tgagaaaccc tatgaatgta aggagtgtgg gaaggccttt
      721 acagtgctcc aagaacttac tcaacatcag agacttcata cgggtgaaaa accctatgaa
      781 tgtaaggaat gtggaaaggc ctttagagta catcagcaac tggctcgaca tcagagaatt
      841 cacactggtg agaaacccta tgaatgtaag gactgtggaa agacctttag acagtgtaca
      901 caccttacac gccatcagag acttcatact gctgaaaagc tctatgaatg taaggaatgt
      961 gggaaagcct tcgtatgtgg tccagacctt agagtacatc agaaaattca ttttggtgag
     1021 aaaccctatg aatgtaagga gtgtggaaag gcttttagaa tatgccaaca acttactgtt
     1081 catcagagta ttcatactgg tgagaaaccc tacgaatgta aggaatgtgg gaagactttt
     1141 agattaagac aacaactagt tcgccatcag agaatacata ctcgtgagaa accctatgaa
     1201 tgtatggaat gttggaagac ctttagtagt tactcacagc ttatttcaca ccagagcatt
     1261 catattggtg agagacccta tgaatgtgaa gagtgtggaa aggcctttag actgctctca
     1321 caacttactc agcatcaaag tattcacact ggtgagaaac cttatgaatg taaggaatgt
     1381 agaaaacctt ttagactgct ctcacaactt acccaacatc agagtattca cactggtgag
     1441 aaaccttatg aatgtaagga atgtggtaag gcttttagac tttattcatt tcttactcaa
     1501 caccagagaa ttcatactgg tgagaagccc tacaagtgta aggagtgtaa gaaggccttt
     1561 agacagcatt cacaccttac tcagcatcag aagattcata atggaattta gtaatgaact
     1621 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     1681 tcccgttata atgcccattt cgt
//
