LOCUS       sequence-282158-        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2117
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcctctgc gccctggccg gagcgggttc cgaggctgct cgctccgcgc
       61 ttaccctctt acccgccccc acccccaaca gtgggattac ggtccatgga gcaggaggag
      121 acctacctgg agctctacct ggaccagtgc gccgctcagg atggccttgc cccacccagg
      181 tctcccctgt tcagcccagt tgtaccttat gatatgtaca tactgaatgc atccaatccg
      241 gatactgcat ttaattcgaa ccctgaagtc aaagaaacat ctggtgattt ctcatctgtg
      301 gatcttagct tcctaccaga tgaagttacc caggaaaata aagaccagcc tgtcattagc
      361 aaacacgaaa ctgaagaaaa ttctgaaagc caaagtccac aaagtaggtt gccatcaccc
      421 agcgaacagg acgttgggct gggcttaaac agcagcagtt tgtcaaattc ccattcacag
      481 ctgcaccctg gtgatactga ctcagtccag ccctctcctg agaaaccaaa ctccgactcc
      541 ttgtctctgg catccataac tcccatgaca ccaatgaccc ctatttcaga atgttgtgga
      601 attgtacctc aactacagaa tatagtttcc actgtaaacc tggcctgtaa gttggatctg
      661 aagaaaatag ctttgcatgc aaaaaatgca gaatataacc caaagaggtt tgctgctgtc
      721 ataatgagga tccgagagcc caggacaaca gcccttatat ttagctctgg gaagatggtc
      781 tgcacgggag ccaaaagtga agagcagtct cgacttgcag caagaaaata tgctcgtgtg
      841 gtgcagaagc ttgggttccc tgccagattc ctcgatttta aaattcagaa catggttgga
      901 agctgtgatg tgagatttcc catcaggctg gaaggtttgg tgctaaccca tcagcagttc
      961 agtagttacg agcctgaact gtttcctggt cttatttata gaatggtaaa accacgaatt
     1021 gtgttgctta tctttgtatc tggaaaagtt gtgttgacag gtgccaaaga acgttctgag
     1081 atctatgaag catttgaaaa catctatcct attctaaaag gttttaaaaa agcctagtaa
     1141 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1201 gacgaccatg gttgctagga ttaatctgc
//
