LOCUS       sequence-282167-        2039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2127
FEATURES             Location/Qualifiers
     source          1..2039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgaggc ggtcgcggct ccgatttctc cgtggacgat ggcagccacg
       61 attcaggcca tggagaggaa gattgaatcg caggctgctc gcctgctttc cctagaaggt
      121 cgaaccggga tggccgagaa gaagctggct gattgcgaga agacagccgt ggagttcggg
      181 aaccagctgg agggcaagtg ggccgtgctg gggaccctgc tgcaggagta cgggctgctg
      241 cagaggcggc tggagaacgt ggagaacctg ctgcgcaaca ggaacttctg gatcctgcgg
      301 ctgcccccgg gcagcaaggg ggagtcccct aaggagtggg gcaagctgga ggactggcag
      361 aaggagctct acaagcacgt gatgaggggc aactacgaga cgctggtctc cctggactac
      421 gccatctcca agcccgaggt cctctcccag attgaacaag ggaaggagcc ctgcaactgg
      481 cgccgccctg gccccaagat tccagatgtt cctgtggacc ccagtccagg ctcggggccc
      541 ccagttcccg ccccagacct cttgatgcag atcaagcagg agggtgagct ccagctccag
      601 gagcagcagg ccctgggcgt ggaggcgtgg gcagccgggc agccagatat tggggaggag
      661 ccctggggcc tcagccagct ggattccgga gcaggagaca tctccacgga tgccacctct
      721 ggtgtccatt ccaacttttc caccaccatc ccgcccacct cctggcaaac ggatctccct
      781 ccccaccatc cctcttcagc atgctcggac gggaccctga agctcaacac agcagcctcc
      841 acggaagcag atgtaaaaat tgtaataaaa acagaagtcc aggaagagga ggtggtggcc
      901 acacccgtac atcctactga cctagaggct cacgggaccc tgtttggacc aggccaagcc
      961 acacggttct tccctagtcc tgcccaggaa ggagcctggg aaagccaggg cagctccttc
     1021 cccagccagg accctgtgct ggggctgcga gagcccgccc ggcctgagag ggacatgggt
     1081 gagctcagtc ctgctgtggc ccaggaggag acccctcctg gggactggct cttcggaggg
     1141 gtccggtggg gctggaattt ccggtgtaaa ccgccagtgg gcctgaaccc gaggacgggg
     1201 cccgaggggc ttccttactc ctccccggac aacggagagg ccatcttgga ccccagccag
     1261 gccccaaggc cattcaacga accctgtaaa taccctggcc ggaccaaagg ctttggccac
     1321 aagccagggc tgaagaagca ccccgcggcg ccccccgggg gccggccctt cacctgcgcc
     1381 acgtgtggga agagcttcca gctgcaagtc agcctgagcg cgcaccagcg cagctgtggg
     1441 gcgcccgacg ggtcgggccc gggcacaggc ggtggcggca gcggcagtgg cggcggcggt
     1501 ggcggcagcg gtgggggcag cgcacgggat ggcagcgccc ttcggtgtgg ggagtgcggc
     1561 cgttgcttca cgcgccccgc gcacctcatc cgccatcgca tgctgcacac cggcgagcgg
     1621 cccttcccct gcaccgagtg tgagaagcgc ttcaccgaac gctccaagct catcgaccac
     1681 taccgaacgc acacgggcgt gcggcccttc acctgcaccg tctgcggcaa aagcttcatc
     1741 cgcaaggacc acctccgcaa gcaccagcgc aaccatgcag cgggcgccaa gaccccggcc
     1801 cgaggccagc cactcccgac gccgcccgca cctcctgatc ccttcaagag ccccgcctcc
     1861 aaaggacctt tggcctccac agaccttgtg accgactgga cttgtggcct cagcgtcctg
     1921 ggacccaccg atggcgggga catgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1981 atttcttggc tttatatatc ttgtggaaag gacgaagtga ctaacgctac gtcgagcct
//
