LOCUS       sequence-282170-        1931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2139
FEATURES             Location/Qualifiers
     source          1..1931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgtggg gctttgtaaa gccatgtccc aggggttggt gaccttcaga
       61 gatgtggcgc tagacttttc ccaagaagag tgggaatggc tgaagccatc tcagaaggat
      121 ttatacagag atgtcatgtt ggagaactac aggaacttgg tatggcttgg actctccatt
      181 tctaagccca acatgatctc cttactggag caagggaagg aaccgtggat ggtggagaga
      241 aagatgtcac agggtcactg tgcagactgg gagtcttggt gtgaaattga ggaattatct
      301 ccaaaatggt tcattgatga agatgaaata tcccaggaga tggtaatgga aaggctagca
      361 agtcatggcc ttgaatgctc cagtttcaga gaagcctgga aatataaggg tgaatttgag
      421 ctacatcagg gaaatgcgga gaggcatttc atgcaagtga cagctgttaa ggaaatctct
      481 actgggaaaa gagacaatga atttagtaat tctgggagaa gcatacccct gaaatcagta
      541 tttttaacac aacagaaagt tcctaccata cagcaagtac ataaatttga tatttatgat
      601 aaactcttcc cccaaaattc agtcataatt gaatataaaa gactccatgc tgagaaggaa
      661 tctttgatag gtaatgaatg tgaagaattc aaccagagta cgtaccttag taaagatata
      721 ggaattcctc ctggggagaa accttatgaa agtcatgatt tttcaaagct cttaagtttc
      781 cactcattat ttactcaaca tcagaccact cattttggaa aattacccca tggatacgat
      841 gaatgtggtg atgcctttag ctgttactca ttctttactc aacctcagag aattcacagt
      901 ggagaaaaac catatgcatg caatgactgt ggaaaagcct ttagccacga cttctttctc
      961 agtgaacatc aaagaactca tattggggag aaaccttatg aatgtaagga atgtaacaaa
     1021 gctttcagac agagtgctca ccttgctcaa catcagagga tccacactgg agagaaaccg
     1081 tttgcgtgca atgaatgtgg gaaggccttt agccgttatg ccttccttgt tgaacatcag
     1141 agaattcaca caggtgagaa accatatgaa tgtaaagaat gtaataaagc cttcagacag
     1201 agtgctcacc ttaatcaaca tcagaggatt cacactggag agaaacccta tgaatgtaat
     1261 cagtgtggaa aagccttcag cagacgcata gcccttactc tacatcaaag aattcacaca
     1321 ggagagaaac ccttcaaatg tagtgaatgt gggaagacct ttggctatcg ctcacacctg
     1381 aatcaacatc agagaattca taccggagaa aagccctatg aatgcatcaa atgtgggaag
     1441 ttttttagga ctgactcaca acttaatcga catcatagaa ttcacactgg agagagacca
     1501 tttgaatgca gtaaatgtgg gaaagccttc agtgatgctt tagttctaat tcaccataag
     1561 agaagtcatg caggagagaa accctatgaa tgtaacaaat gtggaaaggc cttcagttgt
     1621 ggctcatatc ttaatcaaca tcaaagaatt catactggag agaaacccta tgaatgtagt
     1681 gaatgtggga aggcttttca tcagatcttg tccctaagac tacaccagag aattcacgct
     1741 ggagaaaaac cttataaatg taacgaatgt gggaataatt ttagctgtgt ctcagccctt
     1801 agacgacatc agagaattca taatagagaa acgctctagt aatgaactag tccgtaactt
     1861 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatgc gtgtgagtat
     1921 agtacgcgtc t
//
