LOCUS       sequence-282173-        1541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2140
FEATURES             Location/Qualifiers
     source          1..1541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcttcc tcagggtcta ttgacattca gggatgtggc catagaattc
       61 tctcaggagg agtggaaatg cctggaccct gctcagagga ctctatacag ggacgtgatg
      121 ctggagaatt ataggaacct ggtctccctg ggtatctctt ccaaatgcac aatgaaggag
      181 ttctcatcaa cagcacaagg caatacagaa gtgatccaca cagggacatt gcaaagacat
      241 gaacgtcatc acattggaga tttttccttc caggaaattg agaaagatat tcataacttt
      301 gagtttcagt ggcaagaaga tgaaagaaat ggccatgaag cacccatgac aaaaatcaaa
      361 aaattgatgg gtagtacaga gcaatatgat cacaggcatg ctggaaacaa gcctattaaa
      421 tatcagcttg gatcaagctt tcattcacat ctgtctgaac tccacatatt tcagcccaaa
      481 gggaaaatta ataatcaagt ggagaagtct atcaacgatg cttcctcggt ttcaacagcc
      541 caaagaattt cttgtaggcc caaaacccat atatctaata actatgggga taattttctg
      601 aattattcat tactcacaca aagacaggaa gtacgcatga gagaaaaatc tttccaatgt
      661 attgagagtg gcaaagcctt taattatagc tcactcttaa ggaaacatca gattattcat
      721 ctaggagaga aacaatataa atgtgatgta tgtgacaagg tctttattcg gaagcgatac
      781 cttgcacgcc atcgtagatg tcacactggt gagaaacctt acaagtgtaa tgagtgtggc
      841 aagtccttca gtcagatgtc atcccttaca tatcatcatc gacttcatac tggagagaaa
      901 ccttacaaat gtgaagaatg tgacaaagct ttcagacaca attcagccct tcaaagacat
      961 aggagaattc atactggaga gaaaccacat aagtgtaatg agtgtggcaa gacctttagt
     1021 cagaagtcat accttgcatg ccatcgtagc attcatactg gaaagaaacc ttacgaatgt
     1081 gaagaatgtg acaaagcttt cagtttcaaa tcaaaccttg aaagtcatag gataactcat
     1141 actggagaga aaccatacaa gtgtaatgat tgtggcaaga ccttcagtca tatgtcaacc
     1201 cttacatgcc atcgtagact tcatactgga gagaaacctt acaagtgtga agaatgtgat
     1261 gaagctttcc gtttcaaatc aagtcttgaa agacatagga gaattcataa tggagagaaa
     1321 ctgtacaaat gtaatgagtg tggcaagacc ttcagtcagg agttatccct tacctgccat
     1381 tgtagacttc atagtggaga gaaaccttgc aagtgtggag aatgtgacaa ggcttacagt
     1441 ttcaaatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1501 tcttgtggaa aggacgactc gtggataact tagtcctgac g
//
