LOCUS       sequence-282176-        1988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2147
FEATURES             Location/Qualifiers
     source          1..1988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccttac tcagggaccc ttgaaattca tggatgtggc catagagttc
       61 tctcaggaag agtggaaatg cctggaccct gcgcagagga ctttatacag ggacgtgatg
      121 ttggagaatt ataggaacct ggtctccctg ggaatctgtc ttcctgacct gagtgttacc
      181 tccatgttag agcaaaagag agatccctgg actctgcaga gtgaagagaa aatagcaaac
      241 gatccagacg gcagggagtg catcaaaggt gtgaacacag agaggagctc taaattggga
      301 agtaatgcag gaaacaagcc ttgtaaaaat caacttggat tcacttttca gttacatctg
      361 agtgatctac agctatttca agctgaaagg aaaatttctg ggtgtaagca ttttgaaaaa
      421 cccgtcagtg acaattcctc agtttcaccg cttgaaaaaa tttcttccag tgtcaaatcc
      481 caccttttaa ataaatatag aaataatttt gatcatgctc cattacttcc acaagaacag
      541 aaagcacaca ttagggaaaa agcttataaa tgtaatgagc acggccaagt ctttagagca
      601 tctgcaagcc ttactaacca agtaatccat aacgcagata atccttacaa atgcagtgaa
      661 tgtggcaagg tctttagttg cagttcaaag cttgtgatac atcgaagaat gcatactgga
      721 gagaagcctt acaaatgtca tgaatgtggc aagctcttca gtagcaattc aaacctttca
      781 caacatcaaa gaattcatac tggagagaag ccttacaaat gtcatgaatg tgacaaggtc
      841 tttcgaagca gttcaaagct tgcacaacat caaagaattc atactggaga gaagccttac
      901 aaatgtcatg aatgtgacaa ggtcttcaat caaattgcac accttgtacg acatcaaaaa
      961 attcatactg gagagaaacc ttacagttgt aataaatgtg gcaaggtctt tagtcgccat
     1021 tcatatctag cagaacatca aacggttcat actggtgaga aaccttacaa atgtgaagaa
     1081 tgtggcaaag cattttcagt gcgttccagc ctcataaccc atcagttaat tcacactgga
     1141 aggaaacctt acaaatgtaa agaatgtgac aaggtctttg ggcgcaagtg tttcctgacc
     1201 tctcatcaga gaattcatac tagagagaga ccttatggat gcagtcagtg tggcaagatc
     1261 tttagtcaga aatcagacct tatacgacat cgaaaaactc atactgatga gaagccttac
     1321 aaatgtaata aatgtggcac agcgtttaga gagttttcag accttactgc ccattttcta
     1381 atccatagtg gagagaaacc ttatgaatgt aaagaatgtg gcaaagtctt caggtacaag
     1441 tcttctctaa ccagtcatca tagaattcat actggagaga agccttacaa atgtaacaga
     1501 tgtggcaagg tcttcagtcg cagttcaaac ctggtatgcc atcagaaaat tcatacagga
     1561 gaaaagcctt acaaatgtaa tcaatgtggc aaggtcttta atcaagcatc ataccttaca
     1621 agacatcaaa taattcatac tggagagagg ccttacagat gtagtaaatg tggcaaagca
     1681 tttcgagggt gttcaggcct tactgcccat cttgcaatcc atactgaaaa gaaatctcat
     1741 gagtgtaaag aatgtggcaa gatcttcact cagaagtctt ccctcaccaa tcaccataga
     1801 attcacattg gagagaaacc ttacaaatgc accctgtgca gtaaggtctt cagtcacaat
     1861 tctgaccttg cacagcatca gagagttcat tcatagtaat gaactagtcc gtaacttgaa
     1921 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagcgggc tttcactact
     1981 ttactaag
//
