LOCUS       sequence-282185-        1829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2180
FEATURES             Location/Qualifiers
     source          1..1829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagcggc tgcgccgagg cgcccgactc agcagggcac tgtgaccttt
       61 gaagatgtgg ctgtgaactt ttcccaggag gagtggtgtc ttcttagtga ggctcagagg
      121 tgcttgtacc gtgatgtgat gctagagaac ctggctctca tatcctcgct gggttgttgg
      181 tgtggatcaa aagatgagga ggcaccttgt aagcagagaa tttctgtaca aagagagtct
      241 cagagcagga ctcctagggc aggtgtttct cctaagaagg ctcacccctg tgaaatgtgt
      301 ggcctcatct tggaggatgt ttttcacttt gctgaccacc aggaaactca tcacaagcag
      361 aagctgaaca ggagtggagc atgtggaaaa aacttggatg acactgcata ccttcatcag
      421 caccagaagc agcatattgg agagaaattc tacagaaaga gtgtcagaga agcatcgttt
      481 gtaaagaaac gtaagctcag ggtgtcacag gagccatttg tcttccgcga gtttgggaag
      541 gacgttctgc ccagttcagg attgtgccaa gaagcagctg ctgtagagaa gacagacagt
      601 gaaactatgc atggcccacc ctttcaggag ggaaaaacta attacagttg tggaaaacgc
      661 acaaaagcct tcagcaccaa acactcagtt attccacacc agaaactttt cactagagat
      721 ggatgttatg tgtgcagtga ttgtggaaaa tcctttagca gatatgtcag cttcagtaat
      781 catcagcgag atcacactgc aaaaggacct tatgattgtg gagagtgtgg gaaatcttat
      841 agtcgaaaga gcagccttat tcaacatcag cgagtccaca ctggaaagac agcttatccc
      901 tgtgaggagt gcgggaaatc ttttagtcag aagggcagcc ttattagcca tcagcgtgtt
      961 cacactggag aaaggcctta tgagtgtaga gaatatggga aatcttttgg tcaaaagggt
     1021 aacctcattc aacatcagca aggtcacact ggagagagag cttatcactg tggggaatgt
     1081 gggaaatctt ttcgtcagaa gttctgcttt attaaccatc agcgtgttca cactggagaa
     1141 aggccttaca agtgtggaga atgtggaaaa tcttttggtc aaaagggcaa cctcgttcaa
     1201 catcagcgag gtcatactgg agaaaggccc tatgagtgca aggaatgtgg gaaatcattt
     1261 aggtacagat cccacctcac tgaacaccag agacttcaca ctggggaaag accttacaat
     1321 tgtagggaat gtgggaaatt atttaacagg aagtatcatc ttctcgttca tgagagagtt
     1381 cacactggag aaaggccata tgcgtgtgag gtatgtggga aattatttgg taataagaac
     1441 tgcgtgacta tacatcagag gattcacact ggagaaaggc cgtatgaatg caatgaatgt
     1501 gggaaatcat ttctttccag ctctgcgctt catgttcata aaagagttca ttctggacaa
     1561 aagccttata agtgcagtga atgtggaaaa tcctttgctg aatgttccag tctcattaaa
     1621 cacaggagaa ttcacactgg agaaaggcct tatgaatgta ccaaatgtgg aaaaacattt
     1681 cagcgaagct ctaccctcct tcatcatcag agttcacaca ggagaaaggc cttatagtaa
     1741 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1801 gacgagttta tggaacctac gaatgccag
//
