LOCUS       sequence-282194-        1361 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2200
FEATURES             Location/Qualifiers
     source          1..1361
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             307..333
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
ORIGIN
        1 accgccacca tgatgtccat gaacagcaag cagcctcact ttgccatgca tcccaccctc
       61 cctgagcaca agtacccgtc gctgcactcc agctccgagg ccatccggcg ggcctgcctg
      121 cccacgccgc cgctgcagag caacctcttc gccagcctgg acgagacgct gctggcgcgg
      181 gccgaggcgc tggcggccgt ggacatcgcc gtgtcccagg gcaagagcca tcctttcaag
      241 ccggacgcca cgtaccacac gatgaacagc gtgccgtgca cgtccacttc cacggtgcct
      301 ctggcgcacc accaccacca ccaccaccac caccaggcgc tcgaacccgg cgatctgctg
      361 gaccacatct cctcgccgtc gctcgcgctc atggccggcg cgggcggcgc gggcgcggcg
      421 gccggcggcg gcggcgccca cgacggcccg gggggcggtg gcggcccggg cggcggcggc
      481 ggcccgggcg gcggccccgg gggaggcggc ggtggcggcc cggggggcgg cggcggcggc
      541 ccgggcggcg ggctcctggg cggctccgcg caccctcacc cgcatatgca cagcctgggc
      601 cacctgtcgc accccgcggc ggcggccgcc atgaacatgc cgtccgggct gccgcacccc
      661 gggctggtgg cggcggcggc gcaccacggc gcggcagcgg cagcggcggc ggcggcggcc
      721 gggcaggtgg cagcggcatc ggcggcggcg gccgtggtgg gcgcagcggg cctggcgtcc
      781 atctgcgact cggacacgga cccgcgcgag ctcgaggcgt tcgcggagcg cttcaagcag
      841 cggcgcatca agctgggcgt gacgcaggcc gacgtgggct cggcgctggc caacctcaag
      901 atcccgggcg tgggctcact cagccagagc accatctgca ggttcgagtc gctcacgctc
      961 tcgcacaaca acatgatcgc gctcaagccc atcctgcagg cgtggctcga ggaggccgag
     1021 ggcgcccagc gcgagaaaat gaacaagcct gagctcttca acggcggcga gaagaagcgc
     1081 aagcggactt ccatcgccgc gcccgagaag cgctccctcg aggcctactt cgccgtgcag
     1141 ccccggccct cgtccgagaa gatcgccgcc atcgccgaga aactggacct caaaaagaac
     1201 gtggtgcggg tgtggttttg caaccagaga cagaagcaga agcggatgaa attctctgcc
     1261 acttactagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1321 tcttgtggaa aggacgaaaa tttcagcacc aaagggtcgg a
//
