LOCUS       sequence-282224-        1403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2930
FEATURES             Location/Qualifiers
     source          1..1403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgagtt ccacccgttc atcgaggcac tgctgcctca cgtccgcgct
       61 ttctcctaca cctggttcaa cctgcaggcg cggaagcgca agtacttcaa gaagcatgaa
      121 aagcggatgt cgaaggacga ggagcgggcg gtgaaggacg agctgctggg cgagaagccc
      181 gagatcaagc agaagtgggc atcccggctg ctggccaagc tgcgcaagga catccggccc
      241 gagttccgcg aggacttcgt gctgaccatc acgggcaaga agcccccctg ctgcgtgctc
      301 tccaaccccg accagaaggg caagatccgg cggattgact gcctgcgcca ggctgacaag
      361 gtgtggcggc tggacctggt catggtgatt ttgtttaagg ggatccccct ggaaagtact
      421 gatggggagc ggctctacaa gtcgcctcag tgctcgaacc ccggcctgtg cgtccagcca
      481 catcacattg gagtcacaat caaagaactg gatctttatc tggcttactt tgtccacact
      541 ccggaatccg gacaatcaga tagttcaaac cagcaaggag atgcggacat caaaccactg
      601 cccaacgggc acttaagttt ccaggactgt tttgtgactt ccggggtctg gaatgtgacg
      661 gagctggtga gagtatcaca gactcctgtt gcaacagcat cagggcccaa cttctccctg
      721 gcggacctgg agagtcccag ctactacaac atcaaccagg tgaccctggg gcggcggtcc
      781 atcacctccc ctccttccac cagcaccacc aagcgcccca agtccatcga tgacagtgag
      841 atggagagcc ctgttgatga cgtgttctat cccgggacag gccgttcccc agcagctggc
      901 agcagccagt ccagcgggtg gcccaacgat gtggatgcag gcccggcttc tctaaagaag
      961 tcaggaaagc tggacttctg cagtgccctc tcctctcagg gcagctcccc gcgcatggct
     1021 ttcacccacc acccgctgcc tgtgcttgct ggagtcagac cagggagccc ccgggccaca
     1081 gcatcagccc tgcacttccc ctccacgtcc atcatccagc agtcgagccc gtatttcacg
     1141 cacccgacca tccgctacca ccaccaccac gggcaggact cactgaagga gtttgtgcag
     1201 tttgtgtgct cggatggctc gggccaggcc accggacagc attcgcaacg acaggcgcct
     1261 cctctgccaa ccggtttgtc agcatcggac cccgggacgg caactttcta gtaatgaact
     1321 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     1381 ggccctccgc gtgcagaacc aag
//
