LOCUS       sequence-282227-        1340 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2326
FEATURES             Location/Qualifiers
     source          1..1340
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctaaaag accgggaccc cctggaagcc gagaaatggg actgttgaca
       61 ttcagagaca tagctataga attctctctg gcggagtggc aatgcctgga tcatgctcag
      121 cagaatttat atagagatgt gatgttagag aactacagaa acctgttctc cctgggtatg
      181 actgtctcta agccagactt gatcgcctgt ctggagcaaa ataaagagcc ccagaatata
      241 aagagaaatg agatggcagc caaacaccca gttacatgtt ctcatttcaa ccaagacctt
      301 cagccagagc agagcataaa agattcactc caaaaagtaa taccaagaac atatggaaaa
      361 tgtggacatg agaatttaca attaaaaaaa tgttgtaaaa gagtagatga gtgtgaggtg
      421 cacaaaggag gttataatga ccttaaccaa tgtttgtcaa atacccaaaa caaaatattt
      481 cagactcata aatgtgtcaa agtcttcagt aaattttcaa attccaatag acacaatgca
      541 agatatactg gaaagaaaca tttgaaatgt aaaaaatatg gcaaatcatt ttgcatgttt
      601 tcacacctaa atcaacatca gataattcat actaaggaga agtcctacaa atgtgaagaa
      661 tgtggcaaat cctttaacca ctcctcaagc ggtactacac ataaaagaat tcttactgga
      721 gagaaaccct acagatgtga ggaatgtggc aaagccttta ggtggccctc aaaccttact
      781 agacataaga gaattcacac tggagagaaa ccctacgcat gtgaagaatg tggccaagcc
      841 tttaggcgct cctcaacact tactaaccac aagagaattc atactggaga gagaccctac
      901 aaatgtgaag aatgtggcaa agcctttagc gtatcctcag ccctcattta ccacaagaga
      961 attcatactg gagagaaacc ctacacatgt gaagaatgtg gcaaagcctt taactgctcc
     1021 tcgactctta agacacataa gataattcat actggagaga aaccctacac atgtgaagaa
     1081 tgtggcagaa cctttaactg ctcctcaact gtaaaggcac ataagagaat tcatactgga
     1141 gagaaaccat acaaatgtga agaatgtgac aaagctttta agtggcattc aagtcttgct
     1201 aaacataaga taattcacac tggagagaaa ccctacaaat gtaaatagta atgaactagt
     1261 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaacag
     1321 tccacattat tccattaagg
//
