LOCUS       sequence-28223-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Khakh BS, Fisher JA, Nashmi R, Bowser DN, Lester HA
  TITLE     An angstrom scale interaction between plasma membrane ATP-gated P2X2
            and alpha4beta2 nicotinic channels measured with fluorescence 
            resonance energy transfer and total internal reflection fluorescence
            microscopy.
  JOURNAL   J Neurosci. 2005 Jul 20. 25(29):6911-20.
  PUBMED    16033901
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2005 Jul 20. 25(29):6911-20."
COMMENT     Sequence Label: pcDNA3.1-P2X2-CFP
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     227..248
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(300..323)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gangncgctc cngagtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
       61 tggtcggggt agcggctgaa gcactgcacg ccccaggtca gggtggtcac gagggtgggc
      121 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
      181 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      241 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catctcgagc
      301 ttgtcgtcat cgtccttgta gtcaagttgg gccaaacctt tggggtccgt ggatgtggag
      361 tcctgttggg aaggctcagg gacaggaggt ctttgtccca tatgctggcc aagtgtgtcc
      421 accacctgct cagtcagagc agagatggag caaggccgag gagactggac agccagtggt
      481 agctctgccc cttctcccaa agttggtcct tcacctgatg gagggctggg tggctgatcc
      541 tgggagtagt gactaggtgg gggagggatc tggcccaaga caagggcaag ggtcacaggc
      601 catctacttg agggatgctt tggagtacgc accttgtcga acttcttatg gctgtagagc
      661 ttgtttttgt tcatgaacgt taacaaaatc cagtcacaca ggaaggagcc caccccgatg
      721 ga
//
