LOCUS       sequence-282236-        1772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2379
FEATURES             Location/Qualifiers
     source          1..1772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgagga ctggctggac tgcccggccc tgggccctgg ctggaagcgc
       61 cgcgaagtct ttcgcaagtc aggggccacc tgtggacgct cagacaccta ttaccagagc
      121 cccacaggag acaggatccg aagcaaagtt gagctgactc gatacctggg ccctgcgtgt
      181 gatctcaccc tcttcgactt caaacaaggc atcttgtgct atccagcccc caaggcccat
      241 cccgtggcgg ttgccagcaa gaagcgaaag aagccttcaa ggccagccaa gactcggaaa
      301 cgtcaggttg gaccccagag tggtgaggtc aggaaggagg ccccgaggga tgagaccaag
      361 gctgacactg acacagcccc agcttcattc cctgctcctg ggtgctgtga gaactgtgga
      421 atcagcttct caggggatgg cacccaaagg cagcggctca aaacgttgtg caaagactgt
      481 cgagcacaga gaattgcctt caaccgggaa cagagaatgt ttaagagccg agggtgtgga
      541 gtatgccggg gctgtcagac ccaagaggat tgtggccatt gccccatctg ccttcgccct
      601 ccccgccctg gtctcaggcg ccagtggaaa tgtgtccagc gacgttgcct acggggtaaa
      661 catgcccgcc gcaagggagg ctgtgactcc aagatggctg ccaggcggcg ccccggagcc
      721 cagccactgc ctccaccacc cccatcacag tccccagagc ccacagagcc gcaccccaga
      781 gccctggccc cctcgccacc tgccgagttc atctattact gtgtagacga ggacgagcta
      841 cagccctaca cgaaccgccg gcagaaccgc aagtgcgggg cctgtgcagc ctgcctacgg
      901 cggatggact gtggccgctg cgacttctgc tgcgacaagc ccaaattcgg gggcagcaac
      961 cagaagcgcc agaagtgtcg ttggcgccaa tgcctgcagt ttgccatgaa gcggctgctg
     1021 cccagtgtct ggtcagagtc tgaggatggg gcaggatcgc ccccacctta ccgtcgtcga
     1081 aagaggccca gctctgcccg acggcaccat cttggcccta ccttgaagcc caccttggct
     1141 acacgcacag cccaaccaga ccatacccag gctccaacga agcaggaagc aggtggtggc
     1201 tttgtgctgc ccccgcctgg cactgacctt gtgtttttac gggaaggcgc aagcagtcct
     1261 gtgcaggtgc cgggccctgt tgcagcttcc acagaagccc tgttgcagga ggcccagtgc
     1321 tctggcctga gttgggttgt ggccttaccc caggtgaagc aagagaaggc ggatacccag
     1381 gacgagtgga caccaggcac agctgtcctg acttctcccg tattggtgcc tggctgccct
     1441 agcaaggcag tagacccagg cctgccttct gtgaagcaag agccacctga cccagaggag
     1501 gacaaggagg agaacaagga tgattctgcc tccaaattgg ccccagagga agaggcagga
     1561 ggggctggca cacccgtgat cacggagatt ttcagcctgg gtggaacccg cttccgagat
     1621 acagcagtct ggttgccaag gtccaaagac cttaaaaaac ctggagctag aaagcagtag
     1681 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1741 aaggacgata gaccttcccg tccgtagcga gc
//
