LOCUS       sequence-282272-        1463 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2839
FEATURES             Location/Qualifiers
     source          1..1463
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagatat acaaacaaat gcggaactga aaagcactca ggagcagtct
       61 gtgcccgcag aaagtgcagc ggttttgaat gactacagtt taaccaaatc tcatgaaatg
      121 gaaaatgtgg acagtggaga aggcccagcc aatgaagatg aagacatagg agatgattca
      181 atgaaagtga aagatgaata cagtgaaaga gatgagaatg ttttaaagtc agaacccatg
      241 ggaaatgcag aagagcctga aatcccttac agctattcaa gagaatataa tgaatatgaa
      301 aacattaagt tggagagaca tgttgtctca ttcgatagta gcaggccaac cagtggaaag
      361 atgaactgcg atgtgtgtgg attatcctgc atcagcttca atgtcttaat ggttcataag
      421 cgaagccata ctgtggagaa accctacaaa tgtgagtttt gtggaaggag ttacaagcag
      481 agaagttccc ttgaggagca caaggagcgc tgccgtacat ttcttcagag cactgaccca
      541 ggggacactg caagtgcgga ggcaagacac atcaaagcag agatgggaag tgaaagagct
      601 ctcgtactgg acagattagc aagcaatgtg gcaaaacgaa aaagctcaat gcctcagaaa
      661 ttcattggtg agaagcgcca ctgctttgat gtcaactata attcaagtta catgtatgag
      721 aaagagagtg agctcataca gacccgcatg atggaccaag ccatcaataa cgccatcagc
      781 tatcttggcg ccgaagccct gcgccccttg gtccagacac cgcctgctcc cacctcggag
      841 atggttccag ttatcagcag catgtatccc atagccctca cccgggctga gatgtcaaac
      901 ggtgcccctc aagagctgga aaagaaaagc atccaccttc cagagaagag cgtgccttct
      961 gagagaggcc tctctcccaa caatagtggc cacgactcca cggacactga cagcaaccat
     1021 gaagaacgcc agaatcacat ctatcagcaa aatcacatgg tcctgtctcg ggcccgcaat
     1081 gggatgccac ttctgaagga ggttccccgc tcttacgaac tcctcaagcc cccgcccatc
     1141 tgcccaagag actccgtcaa agtgatcaac aaggaagggg aggtgatgga tgtgtatcgg
     1201 tgtgaccact gccgcgtcct cttcctggac tatgtgatgt tcacgattca catgggctgc
     1261 cacggcttcc gtgacccttt cgagtgtaac atgtgtggat atcgaagcca tgatcggtat
     1321 gagttctcgt ctcacatagc cagaggagaa cacagagccc tgctgaagta gtaatgaact
     1381 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     1441 aattcgctct taagtctcgg aca
//
