LOCUS       sequence-282278-        1682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2841
FEATURES             Location/Qualifiers
     source          1..1682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaacaga ggctattgat ggctatataa cgtgtgacaa tgagctttca
       61 cccgaaaggg agcactccaa tatggcaatt gacctcacct caagcacacc caatggacag
      121 catgcctcac caagtcacat gacaagcaca aattcagtaa agctagaaat gcagagtgat
      181 gaagagtgtg acaggaaacc cctgagccgt gaagatgaga tcaggggcca tgatgagggt
      241 agcagcctag aagaacccct aattgagagc agcgaggtgg ctgacaacag gaaagtccag
      301 gagcttcaag gcgagggagg aatccggctt ccgaatggta aactgaaatg tgacgtctgt
      361 ggcatggttt gcattgggcc caatgtgctt atggtacata aaaggagtca cactggtgaa
      421 cgccccttcc actgtaacca gtgtggagct tcttttactc agaagggcaa ccttctgaga
      481 cacataaagt tacactctgg agagaagccg ttcaaatgtc ctttctgtag ctacgcctgt
      541 agaagaaggg acgccctcac aggacacctc aggacccatt ctgtgggtaa acctcacaag
      601 tgcaactact gtggacgaag ctacaagcag cgcagttcac tggaggagca caaggaacgc
      661 tgccacaact atctccagaa tgtcagcatg gaggctgctg ggcaggtcat gagtcaccat
      721 gtacctccta tggaagattg taaggaacaa gagcctatta tggacaacaa tatttctctg
      781 gtgccttttg agagacctgc tgtcatagag aagctcacgg ggaatatggg aaaacgtaaa
      841 agctccactc cacaaaagtt tgtgggggaa aagctcatgc gattcagcta cccagatatt
      901 cactttgata tgaacttaac atatgagaag gaggctgagc tgatgcagtc tcatatgatg
      961 gaccaagcca tcaacaatgc aatcacctac cttggagctg aggcccttca ccctctgatg
     1021 cagcacccgc caagcacaat cgctgaagtg gccccagtta taagctcagc ttattctcag
     1081 gtctatcatc caaataggat agaaagaccc attagcaggg aaactgctga tagtcatgaa
     1141 aacaacatgg atggccccat ctctctcatc agaccaaaga gtcgacccca ggaaagagag
     1201 gcctctccca gcaatagctg cctggattcc actgactcag aaagcagcca tgatgaccac
     1261 cagtcctacc aaggacaccc tgccttaaat cccaagagga aacaaagccc agcttacatg
     1321 aaggaggatg tcaaagcttt ggatactacc aaggctccta agggctctct gaaggacatc
     1381 tacaaggtct tcaatggaga aggagaacag attagggcct tcaagtgtga gcactgccga
     1441 gtccttttcc tagaccatgt catgtacacc attcacatgg gttgccatgg ctaccgggac
     1501 ccactggaat gcaacatctg tggctacaga agccaggacc gttatgagtt ttcatcacac
     1561 attgttcgag gggagcacac attccactag taatgaacta gtccgtaact tgaaagtatt
     1621 tcgatttctt ggctttatat atcttgtgga aaggacgagc tgcaatgtac ggcggataag
     1681 cc
//
