LOCUS       sequence-282281-        1604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2842
FEATURES             Location/Qualifiers
     source          1..1604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaacaga ggctattgat ggctatataa cgtgtgacaa tgagctttca
       61 cccgaaaggg agcactccaa tatggcaatt gacctcacct caagcacacc caatggacag
      121 catgcctcac caagtcacat gacaagcaca aattcagtaa agctagaaat gcagagtgat
      181 gaagagtgtg acaggaaacc cctgagccgt gaagatgaga tcaggggcca tgatgagggt
      241 agcagcctag aagaacccct aattgagagc agcgaggtgg ctgacaacag gaaagtccag
      301 gagcttcaag gcgagggagg aatccggctt ccgaatggtg aacgcccctt ccactgtaac
      361 cagtgtggag cttcttttac tcagaagggc aaccttctga gacacataaa gttacactct
      421 ggagagaagc cgttcaaatg tcctttctgt agctacgcct gtagaagaag ggacgccctc
      481 acaggacacc tcaggaccca ttctgtgggt aaacctcaca agtgcaacta ctgtggacga
      541 agctacaagc agcgcagttc actggaggag cacaaggaac gctgccacaa ctatctccag
      601 aatgtcagca tggaggctgc tgggcaggtc atgagtcacc atgtacctcc tatggaagat
      661 tgtaaggaac aagagcctat tatggacaac aatatttctc tggtgccttt tgagagacct
      721 gctgtcatag agaagctcac ggggaatatg ggaaaacgta aaagctccac tccacaaaag
      781 tttgtggggg aaaagctcat gcgattcagc tacccagata ttcactttga tatgaactta
      841 acatatgaga aggaggctga gctgatgcag tctcatatga tggaccaagc catcaacaat
      901 gcaatcacct accttggagc tgaggccctt caccctctga tgcagcaccc gccaagcaca
      961 atcgctgaag tggccccagt tataagctca gcttattctc aggtctatca tccaaatagg
     1021 atagaaagac ccattagcag ggaaactgct gatagtcatg aaaacaacat ggatggcccc
     1081 atctctctca tcagaccaaa gagtcgaccc caggaaagag aggcctctcc cagcaatagc
     1141 tgcctggatt ccactgactc agaaagcagc catgatgacc accagtccta ccaaggacac
     1201 cctgccttaa atcccaagag gaaacaaagc ccagcttaca tgaaggagga tgtcaaagct
     1261 ttggatacta ccaaggctcc taagggctct ctgaaggaca tctacaaggt cttcaatgga
     1321 gaaggagaac agattagggc cttcaagtgt gagcactgcc gagtcctttt cctagaccat
     1381 gtcatgtaca ccattcacat gggttgccat ggctaccggg acccactgga atgcaacatc
     1441 tgtggctaca gaagccagga ccgttatgag ttttcatcac acattgttcg aggggagcac
     1501 acattccact agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1561 atatcttgtg gaaaggacga cgttgacgat catcgaacgt ctga
//
