LOCUS       sequence-282302-        2222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2904
FEATURES             Location/Qualifiers
     source          1..2222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagtgcc ggcggctttg atccctccga cccagctggt cccccctcaa
       61 cccccaatct ccacgtctgc ttcctcctct ggcaccacca cctccacctc ttcggcgact
      121 tcgtctccgg ctccttccat cggacccccg gcgtcctctg ggccaactct gttccgcccg
      181 gagcccatcg cttcggcggc ggcggcggcg gccacagtca cctctaccgg cggcggcggc
      241 ggcggcggcg gcagcggagg cggcggcggc agcagcggca acggaggcgg cggtggcggc
      301 ggcggcggtg gcagcaactg caaccccaac ctggcggccg cgagcaacgg cagcggcggc
      361 ggcggcggcg gcatcagcgc tggcggcggc gtcgcttcca gcacccccat caacgccagc
      421 accggcagca gcagcagcag cagtagcagc agcagcagca gcagcagtag tagcagcagc
      481 agcagtagca gcagcagctg cggccccctc cccgggaaac ccgtgtactc aaccccgtcc
      541 ccagtggaaa acacccctca gaataatgag tgcaaaatgg tggatctgag gggggccaaa
      601 gtggcttcct tcacggtgga gggctgcgag ctgatctgcc tgccccaggc tttcgacctg
      661 ttcctgaagc acttggtggg gggcttgcat acggtctaca ccaagctgaa gcggctggag
      721 atcacgccgg tggtgtgcaa tgtggaacaa gttcgcatcc tgaggggact gggcgccatc
      781 cagccaggag tgaaccgctg caaactcatc tccaggaagg acttcgagac cctctacaat
      841 gactgcacca acgcaagttc tagacctgga aggcctccta agaggactca aagtgtcacc
      901 tccccagaga actctcacat catgccgcat tctgtccctg gtctcatgtc tcctgggata
      961 attccaccaa caggtctgac agcagccgct gcagcagctg ctgctgctac caatgcagct
     1021 attgctgaag caatgaaggt gaaaaaaatc aaattagaag ccatgagcaa ctatcatgcc
     1081 agtaataacc aacatggagc agactctgaa aacggggaca tgaattcaag tgtcggactg
     1141 gaacttcctt ttatgatgat gccccaccct ctaattcctg tcagcctacc tccagcatct
     1201 gtcaccatgg caatgagcca gatgaaccac ctcagcacca ttgcaaatat ggcagcagca
     1261 gcacaagttc agagtccccc atccagagtt gagacatcag ttattaagga gcgtgttcct
     1321 gatagcccct cacctgcccc ctctctggag gaggggagaa ggcctggcag tcacccatca
     1381 tcacatcgca gcagcagcgt gtccagctcc cctgctcgga ctgagagctc ttctgacaga
     1441 atcccggtcc atcagaatgg gttgtccatg aaccagatgc tgatgggctt atcaccaaat
     1501 gtacttcctg ggcccaaaga gggagatttg gccggtcatg acatgggaca tgagtcaaaa
     1561 aggatgcata ttgaaaaaga tgagaccccg ctttctacac caaccgcaag agacagcctt
     1621 gacaaactct ctctaactgg gcatggacaa ccactgcctc caggttttcc atctcctttt
     1681 ctgtttcctg atggactgtc ttccatcgag actcttctga ctaacataca ggggctgttg
     1741 aaagttgcca tagataatgc cagagctcaa gagaaacagg tccaactgga aaaaactgag
     1801 ctgaagatgg attttttaag ggaaagagaa ctaagggaaa cacttgagaa gcagttggct
     1861 atggaacaaa agaatagagc catagttcaa aagaggctaa agaaggagaa gaaggcaaag
     1921 agaaaattgc aggaagcact tgagtttgag acgaaacggc gtgaacaagc agaacagacg
     1981 ctaaaacagg cagcttcaac agatagtctc agggtcttaa atgactctct gaccccagag
     2041 atagaggctg accgcagtgg cggcagaaca gatgctgaaa ggacaataca agatggaaga
     2101 ctgtatttga aaactactgt catgtactag taatgaacta gtccgtaact tgaaagtatt
     2161 tcgatttctt ggctttatat atcttgtgga aaggacgagg cttatagtcg taggcgggtg
     2221 cc
//
