LOCUS       sequence-282305-        2027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0008
FEATURES             Location/Qualifiers
     source          1..2027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggactcagt ggtctttgag gatgtggctg tgaacttcac ccaggaggag
       61 tgggctttgc tgggtccctc tcagaagaaa ctctacagag atgtgatgca agaaaccttt
      121 gttaacttgg cctctatagg ggaaaactgg gaggagaaga acattgaaga tcacaaaaat
      181 caggggagaa agctaagaag tcatatggta gagaggctct gtgaaaggaa agaaggtagt
      241 cagtttggag aaaccatcag tcagactcca aatcctaaac caaacaagaa aacttttact
      301 agagtaaaac catatgaatg tagtgtgtgt ggaaaggact atatgtgtca ttcatctctt
      361 aataggcaca tgagatctca tactgaacat agatcatatg aatatcacaa atatggagag
      421 aaatcatatg aatgtaagga atgtgggaaa agattcagct ttcgaagttc atttcgaata
      481 catgaaagaa ctcacactgg agagaaaccc tataaatgta aacagtgtgg taaggctttc
      541 agttggccca gttcctttca aatacatgaa agaactcata ctggagagaa accttatgaa
      601 tgtaaggaat gtgggaaggc cttcatttat cacacaacct ttcgaggaca catgagaatg
      661 cacacagggg agaaacccta taaatgtaaa gaatgcggga aaacgttcag tcatcccagt
      721 tcttttcgaa atcatgaaag aactcactct ggagagaaac cctatgaatg taaacaatgt
      781 ggaaaagctt tcagatatta ccaaactttt caaatacatg aaaggactca cactggggaa
      841 aaaccctatc agtgtaagca atgtggtaaa gctcttagtt gtcccacatc ctttcgaagt
      901 catgaaagga ttcacactgg agaaaaaccc tataaatgta aaaaatgtgg gaaagccttc
      961 agttttccta gttcctttag aaaacatgaa agaattcata caggagagaa accctatgat
     1021 tgtaaggaat gtgggaaagc attcatttct cttccaagct atcgaagaca tatgataatg
     1081 cacactggaa atggacctta taaatgcaag gaatgtggga aagcctttga ttgtcctagt
     1141 tcttttcaaa tccatgaacg aactcacact ggagagaaac cctatgaatg taaacagtgt
     1201 ggtaaagcct tcagttgttc cagttccttt cgaatgcatg aaagaactca cactggagag
     1261 aaaccccatg aatgtaaaca atgtggtaaa gccttcagtt gttccagttc tgttcgaata
     1321 catgaaagga ctcacactgg agagaaaccc tatgaatgta aacagtgtgg taaagccttc
     1381 agttgttcca gttcctttcg aatgcatgaa agaattcaca ctggagagaa accctatgaa
     1441 tgtaaacagt gtggtaaagc ctttagtttt tctagttcct ttcggatgca tgaaaggact
     1501 cacactggag agaaacccta tgaatgtaaa caatgtggta aagccttcag ttgttccagt
     1561 tcctttcgaa tgcatgaaag gactcacact ggggagaaac cctatgaatg taaacagtgt
     1621 ggtaaggcgt ttagttgttc cagttccatt cgaatacatg aaaggactca cactggagag
     1681 aaaccttatg agtgtaaaca atgtggtaag gccttcagtt gttctagttc tgttcgaatg
     1741 catgaaagga ctcacactgg agtgaaaccc tatgaatgta aacaatgtga caaagccttc
     1801 agttgctcac gttcctttcg aatccatgaa cgaactcaca ctggagagaa accctatgca
     1861 tgtcaacaat gtggtaaagc cttcaagtgt tcccgttcct ttcgaataca tgaaagagtt
     1921 catagtggag agtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1981 tatatatctt gtggaaagga cgataccacg tgtgcaggat ccgcggg
//
