LOCUS       sequence-282308-        1322 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0181
FEATURES             Location/Qualifiers
     source          1..1322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagtctct tcgcgggtac acccacagtg atattggcta ccgcagcctg
       61 gcagtcggcg aggacatcga ggaagtgaat gatgagaaac ttaccgtgac ctcgctgatg
      121 gcaaggggag gagaggacga ggagaataca cggtccaaac ctgagtacgg aacagaagcg
      181 gaaaacaacg ttggcacgga agggtccgtc ccctcggacg accaagaccg tgagggtggc
      241 ggcggccacg agccggagca acagcaggag gagccgcccc tgaccaagcc ggagcaacag
      301 caggaggagc cgcccctgct cgagctgaag caagagcagg aggagccgcc ccagacgacc
      361 gtggaggggc cacagccggc ggaggggcca caaaccgctg agggaccaca gcccccagag
      421 aggaaacgcc gccgccgcac cgcgttcacg cagtttcagc tgcaggagct agagaacttt
      481 ttcgatgaat ctcaatatcc cgacgttgtg gcgcgagaga gacttgcagc acgcctgaat
      541 ttgactgaag acagagtgca ggtttggttt cagaacagaa gagccaagtg gaaacgaaat
      601 cagagggtgc taatgttgag aaacactgct actgctgacc tggcccaccc tttggacatg
      661 ttcttgggtg gggcctatta tgctgctcct gctctggatc ctgctttgtg tgttcatctg
      721 gtgccacaac tacctagacc acctgtgctg cctgtgccac ctatgccacc caggccaccc
      781 atggtcccta tgccacccag gccacccata gcacctatgc cacccatggc gcctgtgcca
      841 cccggctcac gcatggcgcc tgtgccaccc gggccacgca tggcgcctgt gccaccctgg
      901 ccgcccatgg cgcctgtgcc accctggccg cccatggcgc ctgtgccaac cgggccgccc
      961 atggcgcctg tgccacccgg gccgcccatg gcgcgtgtgc cacccgggcc gcccatggcg
     1021 cgtgtgccac ccgggccgcc catggcgcct ctgccacccg ggccgcccat ggcgcctctg
     1081 ccacccgggc cgcccatggc gcctctgcca cccgggccgc ccatggcgcc tctgccaccc
     1141 aggtcacatg tgcctcacac tggcctggct cctgtacaca tcacatgggc ccctgtcatc
     1201 aacagttatt atgcatgtcc ctttttctag taatgaacta gtccgtaact tgaaagtatt
     1261 tcgatttctt ggctttatat atcttgtgga aaggacgagg cagggataca ttacacatgt
     1321 gc
//
