LOCUS       sequence-282319-       13981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13981)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0323
FEATURES             Location/Qualifiers
     source          1..13981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    9838..10426
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9891..9911)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10309..10320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10498..10731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10809..10930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10846..10865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10900..10919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10970..11105
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11125..11144)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11126..11144)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11154..11171)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11154..11170)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11163..11185)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11312..11767
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11399..11418)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11609..11630
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11793..11897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11898..11966,11967..12758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12116..12135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12929..13517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13418..13437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13671..13688
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13805..13826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13841..13846,13847..13864,13865..13871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13879..13895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13884..13906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13903..13919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13903..13919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13938..13958
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13940..13958
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggac aagggaagag agagaatggc
     4141 cgctgccgcc gccgctgctg ccgccgccgc cgccgccgct cagtgccgga gccctcggtg
     4201 cgcggcggag agaagaggat tccggcggga actcgactct tggcgccacc gcctcatgca
     4261 ctgtgtaggt tttgagagta ttttagaagg gctttatgga ccacggctac gaagagacct
     4321 cagtttattt gaagactgtg aaccagaaga gctgactgac tggtctatgg atgaaaaatg
     4381 ttcattttgt aacctacaga gagaagcagt cagtgattgt ataccatctc ttgattcttc
     4441 acagtcaaca ccaacagagg agctatcatc tcagggccag tccaacactg ataagattga
     4501 atgccaagca gaaaattacc taaatgcact ctttcgaaag aaagatcttc ctcagaactg
     4561 tgatcctaac attcccctag ttgctcagga attaatgaaa aagatgatac gtcaatttgc
     4621 gattgagtac atttcaaaaa gtggtaaaac tcaagagaat agaaatggtt caattggacc
     4681 aagtatagta tgtaaaagta tccaaatgaa tcaagcagaa aactcccttc aggaagagca
     4741 ggaaggcccc ttagacctca ctgtgaatcg aatgcaagaa caaaatactc agcaagggga
     4801 tggagtgtta gatctctcta caaagaaaac cagcataaaa tctgaagagt catccatatg
     4861 tgatccttct tctgaaaatt cagtggctgg ttcaactgtt gatgcaaaat cagaggaagc
     4921 tactaaaatg gaaaaaagaa aatcagcatt aaacaaagtt ttggaatctt tgtgcataca
     4981 tcaccagcaa caagttttgg ctatgttgaa atttctagtc caagagcaga atgctgcttc
     5041 tctttgctgt tgtaatacat catgtgttgt gtcttcagaa tctcaaaagc ccttaatcga
     5101 agatgattta tgtggtctgt tctgtagttg tgaatatagg ctggcagaaa gaggttgttt
     5161 acaaaacgaa aaacaaagcc ctggtttaga acctctgcca gtctgtatta aagatttaca
     5221 ttgtttatct tgccaaactg taactgttga acacgttaag ccagtagtga atagaggaat
     5281 tgcagacagt tataattctc acaggtgctg ttctggactg ttaccaaaca ttcactctac
     5341 aaaatcagcc tttcgtagtc ctcttttgtc aaaggaagta tgtgatcttt cagtcactct
     5401 taaagatgcc tgtagatctc gaagtccctc acccccacca ttatcaccta tagaaactga
     5461 aggatttgaa aaattgaaag acgtcatctc agagatttca gccttagaaa ataacaaact
     5521 tgaagcaaac attaaccagc ctccatctct cacaccagca gaaatcagca gcaataagag
     5581 tgatcatcaa gataaaatac ttaaaactaa aaaatccagt aactcttatt ctttactctc
     5641 aaatgacagc aataattcta ctacaaatca tgaaaaaggt gaaactgctg taatttttca
     5701 agatttaatg gatcgcatta atgaaaaact aaaatcaata gaaaccacgg atatgacaag
     5761 ccttgtaaaa ttatctagca gtgattataa tacatataat gatttaaaat tgggagattt
     5821 cataacatct ctcttgcata atgcaaaagc cagtgattat agttttatgg aattattgag
     5881 tcaacatgat aaaaaggtag aaaataaaat tattcagaca agatttcgaa agcgtcaaga
     5941 aactttattt gcaatgcgca actcctctga ttcacccatg tttagaaggc agtctttaca
     6001 gataaaaaga gaacttgcta gtcttgatga aaattttaca agaaaaaaat acaccgaaaa
     6061 aagttcaagg aaattgacac aaaacaatga gatatcttca tcagacaaag gagaattcta
     6121 tcatgaccaa gggccttctt tacaaaattc taaaagactt caaggtaaaa atcatgctga
     6181 aacatcattt tcaccaaatt atgcattaca gtcactgcaa ctacctcttc atagttcaga
     6241 aactaacttg tcttttaatg aattttcaga aagctttaaa acaacttccc ctgagaaaat
     6301 gagcataaga aagccacagg agaaatctgc agatggaaaa caatttttgc aaaatgatag
     6361 gaaaaatcca aagttaggta atactcaaac tcttttgaga aatgatgttt ctggactttt
     6421 gagcagaact aaacgaaata ttgtgcctcc agggtggtat tctgtatatg taacaaataa
     6481 ttatgttttt aaaaaatccc ctaaagccaa aaaagtatcc gaatccacaa caaaaaacga
     6541 tccagtgaaa aatattcaca ttgaaagctc acacaatata gacctaaaca aaattgcaat
     6601 gaattctaat ttacaagtgg ttgtgaagcg tttggaagat acaataagta tagccaaaaa
     6661 atcctggaat aatcagccat tatcagaagg atataaagca tccaagaaat tgatagaaat
     6721 tgatggtaaa gaccaacatg ctgacagaaa tatgactctt actctaaata gaatgacatg
     6781 caaagaacag agcttatcaa aatctgtggt agcatccggc aatattatca atagtcactg
     6841 catgcctaca gtggatttga ataacaaaag acttgaaaat ctgaaaaagt catctatttt
     6901 agatatgggt cgcttgattt ccagtgttga aaatgtacca gcaaaatatg aaggtactga
     6961 aagttcatct gtttccaact attctagtcc tatcaaactc atgtttttat ctgaggttaa
     7021 aagtagtgaa ggggtcaaat atactttaac ttcagttggc acttcccatt caaatgttgt
     7081 tctcccttct gaaaaaccta caacccatca cgtaactgaa gaaaaaacag aaacaaatga
     7141 ggatatctca aatgcgaact ctgaaaatta tcactccagt cattatgata ctgatacttt
     7201 tcaaagagaa ctaaacaaat tcagtcatgc aaaagaaact gcaggatcct ctacaatgtt
     7261 tataggtgat ataaatagtg ataagccaca agaagaacct aaggacaatt caagcagtgc
     7321 tactgatcca tcttttaaaa gaaaaccagg tagaccaaaa aaaataggtc cccaagttgt
     7381 gaaacagatt aagcgaccaa ttggaagacc accaaagcct aaaattgatc aaacaaacat
     7441 cactgtttgc cagaatgagc cctttagtgc tggaaggaaa agcccagaat gtctcatatc
     7501 agaagtaaaa gaaggtattt ataaaaagag tattacagta actgttattt acggaaggtc
     7561 aagaagaact aaaaggcatg tttctgaagg aagggtaaac ataagcaacc ttatgtcttt
     7621 aaacaataat gctggtgatt ttccaactga atataatagt cccagaaata ttagtgaaga
     7681 caaaattgac ttgggtgaaa gaataagtgc tgtagcaagc ttgactactg aaagtgagat
     7741 cttggggtct ggctttgaat atgtgagacc catcaagaac aaatctgtga tacctcagcc
     7801 ttccaagaac attattcgac caaatcagaa gcctttggca ataagtagga agcctggtag
     7861 accggcaaaa gtgaaaatct ctggcatatc tgtgactatt aatagaactt cacctcagga
     7921 aagagaagta agtattagca gctgcttgcc tcctttagaa caggataata cgtcaggaaa
     7981 aaatctgcct gaagaaaagt atgaccaaca gtgcactcaa atggataaaa taaggcacac
     8041 tgaagctgat atatttaaaa atggatcaaa acgtatgatt gctactgtac ctttgagaca
     8101 ttctattagg gatagaaaac catctctgca tttcttacat tcattagcat cttctagctc
     8161 acttatttat agaaacgctc tgctccataa atcatataaa ctgcatttgc agaaaaataa
     8221 aagtcagaag gaaaaacata ggcactcaaa gatgaaaata gcttacaaag ataccccaag
     8281 aaacagactt tcgcggaatg caaaaaagtg tttggaagat aataaattag tacctatttc
     8341 tgaagtatcc ttggatccta taatttcatc aaaccctttg ctcaggtggt gggctacttc
     8401 tgcttcaaat gattccttat tagaggaatt aaacaataga tttgagcaaa taacaaatgc
     8461 ttgggtgcaa gtaagtggag atgaagctga aaattgtatt cataaaaaaa gagaacacat
     8521 tgaaaatgat catttcaaag tagcaagccc tttggaaact tgtcttttag aacttgaagt
     8581 ttcacctgta aaaatgcttt ttcagaaaaa gtatgatttg aatgaactct gtacttggtt
     8641 tatgcaaaca acagaaacac agtctctttc actagttaga aaagcaaatg cccgaaaccc
     8701 tttggaagta ataaatacca ggggaatcaa attagggacc aaatattctg actttaatgc
     8761 cagccccttc agaaagcact ttaaaaaatt tgcactctct tctccttcaa aatcagcaga
     8821 gaaattgcat atactgcata aagtggctaa ctctccactc ttaaatgtga aaagtaattt
     8881 agcaatagct agattaaaaa ggactgagtt taagaggttg catcatgaaa ggtggaaaag
     8941 agagggaaag ctgcacaacc atggaacagt tgactggaac tctaaaagga gaaacttaag
     9001 atttttctgc cagaaccaat ttttaaataa gactgaggga gaaacaaatg ctgacatccc
     9061 actccaagga aaaagcatag tagataatca gtgtgttttg ccacctgaga tcaggggtga
     9121 cttgcagcag agggtagtaa tgcctgactt caaaatacat gctagttttg agaataaatt
     9181 taagtcagaa gcaaaagaga atggaacaaa ttgcagccaa aaagactttc aaaagggacc
     9241 aagactagaa aatgtatgtc ctaatagttg gaggtcaaaa accttaaaag actgtagaat
     9301 atttttgagg aagctcaact gtcttgaaca cagaaatact tttaagctaa atacaatcat
     9361 ttactctcct gaatctactg acagtggaaa tactcatcag actcatatgg aagaatcaaa
     9421 gcgctttact ttaagatctc attctgctag gcaaaattct tttaaaaagc aatctaaaga
     9481 aatagaaaat gctaacacaa ataatccttc agctgatgaa tttgctgacc atcttggcaa
     9541 tagtaaatta agcaaatgtg ttaactttga caagaatcct gatagttttg aagttcttag
     9601 caatttgaac aaaagaaaaa gaccaccatg gaagatcaca gaaatgtcaa caaaaagaca
     9661 taaacgacag tcttgcaaca gtggacaaat ggcaaactat ttttccaaat ccctagcatg
     9721 ctacaagtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     9781 atcttgtgga aaggacgagt tatcgggcct cgcgggatgc gcacgcgtta agtcgacaat
     9841 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     9901 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     9961 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
    10021 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
    10081 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
    10141 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
    10201 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
    10261 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
    10321 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
    10381 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
    10441 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
    10501 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
    10561 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    10621 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    10681 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
    10741 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
    10801 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    10861 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10921 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
    10981 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    11041 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
    11101 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
    11161 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
    11221 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    11281 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
    11341 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    11401 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    11461 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    11521 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    11581 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    11641 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    11701 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    11761 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    11821 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    11881 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    11941 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    12001 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    12061 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    12121 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    12181 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    12241 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    12301 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    12361 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    12421 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    12481 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    12541 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    12601 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    12661 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    12721 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    12781 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    12841 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    12901 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    12961 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    13021 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    13081 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    13141 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    13201 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    13261 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    13321 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    13381 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    13441 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    13501 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    13561 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    13621 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    13681 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    13741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    13801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    13861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    13921 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    13981 c
//
