LOCUS       sequence-28232-e         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Qian F, Boletta A, Bhunia AK, Xu H, Liu L, Ahrabi AK, Watnick TJ, 
            Zhou F, Germino GG
  TITLE     Cleavage of polycystin-1 requires the receptor for egg jelly domain 
            and is disrupted by human autosomal-dominant polycystic kidney 
            disease 1-associated mutations.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Dec 24. 99(26):16981-6.
  PUBMED    12482949
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Dec 24. 99(26):16981-6."
COMMENT     Sequence Label: WT (AF20)
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             470..493
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(564..583)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggagttccgc cacaaagtcc gctttgaagg gatggagccg ctgccctctc gctcctccag
       61 gggctccaag gtatccccgg atgtgccccc acccagcgct ggctccgatg cctcgcaccc
      121 ctccacctcc tccagccagc tggatgggct gagcgtgagc ctgggccggc tggggacaag
      181 gtgtgagcct gagccctccc gcctccaagc cgtgttcgag gccctgctca cccagtttga
      241 ccgactcaac caggccacag aggacgtcta ccagctggag cagcagctgc acagcctgca
      301 aggccgcagg agcagccggg cgcccgccgg atcttcccgt ggcccatccc cgggcctgcg
      361 gccagcactg cccagccgcc ttgcccgggc cagtcggggt gtggacctgg ccactggccc
      421 cagcaggaca ccccttcggg ccaagaacaa ggtccacccc agcagcactg actacaagga
      481 cgacgatgac aagtgatcag gtagcttatc gataccgtcg acccgggcgg ccgccgcttc
      541 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      601 aaaaatgctt tatttgtgaa atttgtgatg cta
//
