LOCUS       sequence-282320-        5717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0323
FEATURES             Location/Qualifiers
     source          1..5717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacaaggg aagagagaga atggccgctg ccgccgccgc tgctgccgcc
       61 gccgccgccg ccgctcagtg ccggagccct cggtgcgcgg cggagagaag aggattccgg
      121 cgggaactcg actcttggcg ccaccgcctc atgcactgtg taggttttga gagtatttta
      181 gaagggcttt atggaccacg gctacgaaga gacctcagtt tatttgaaga ctgtgaacca
      241 gaagagctga ctgactggtc tatggatgaa aaatgttcat tttgtaacct acagagagaa
      301 gcagtcagtg attgtatacc atctcttgat tcttcacagt caacaccaac agaggagcta
      361 tcatctcagg gccagtccaa cactgataag attgaatgcc aagcagaaaa ttacctaaat
      421 gcactctttc gaaagaaaga tcttcctcag aactgtgatc ctaacattcc cctagttgct
      481 caggaattaa tgaaaaagat gatacgtcaa tttgcgattg agtacatttc aaaaagtggt
      541 aaaactcaag agaatagaaa tggttcaatt ggaccaagta tagtatgtaa aagtatccaa
      601 atgaatcaag cagaaaactc ccttcaggaa gagcaggaag gccccttaga cctcactgtg
      661 aatcgaatgc aagaacaaaa tactcagcaa ggggatggag tgttagatct ctctacaaag
      721 aaaaccagca taaaatctga agagtcatcc atatgtgatc cttcttctga aaattcagtg
      781 gctggttcaa ctgttgatgc aaaatcagag gaagctacta aaatggaaaa aagaaaatca
      841 gcattaaaca aagttttgga atctttgtgc atacatcacc agcaacaagt tttggctatg
      901 ttgaaatttc tagtccaaga gcagaatgct gcttctcttt gctgttgtaa tacatcatgt
      961 gttgtgtctt cagaatctca aaagccctta atcgaagatg atttatgtgg tctgttctgt
     1021 agttgtgaat ataggctggc agaaagaggt tgtttacaaa acgaaaaaca aagccctggt
     1081 ttagaacctc tgccagtctg tattaaagat ttacattgtt tatcttgcca aactgtaact
     1141 gttgaacacg ttaagccagt agtgaataga ggaattgcag acagttataa ttctcacagg
     1201 tgctgttctg gactgttacc aaacattcac tctacaaaat cagcctttcg tagtcctctt
     1261 ttgtcaaagg aagtatgtga tctttcagtc actcttaaag atgcctgtag atctcgaagt
     1321 ccctcacccc caccattatc acctatagaa actgaaggat ttgaaaaatt gaaagacgtc
     1381 atctcagaga tttcagcctt agaaaataac aaacttgaag caaacattaa ccagcctcca
     1441 tctctcacac cagcagaaat cagcagcaat aagagtgatc atcaagataa aatacttaaa
     1501 actaaaaaat ccagtaactc ttattcttta ctctcaaatg acagcaataa ttctactaca
     1561 aatcatgaaa aaggtgaaac tgctgtaatt tttcaagatt taatggatcg cattaatgaa
     1621 aaactaaaat caatagaaac cacggatatg acaagccttg taaaattatc tagcagtgat
     1681 tataatacat ataatgattt aaaattggga gatttcataa catctctctt gcataatgca
     1741 aaagccagtg attatagttt tatggaatta ttgagtcaac atgataaaaa ggtagaaaat
     1801 aaaattattc agacaagatt tcgaaagcgt caagaaactt tatttgcaat gcgcaactcc
     1861 tctgattcac ccatgtttag aaggcagtct ttacagataa aaagagaact tgctagtctt
     1921 gatgaaaatt ttacaagaaa aaaatacacc gaaaaaagtt caaggaaatt gacacaaaac
     1981 aatgagatat cttcatcaga caaaggagaa ttctatcatg accaagggcc ttctttacaa
     2041 aattctaaaa gacttcaagg taaaaatcat gctgaaacat cattttcacc aaattatgca
     2101 ttacagtcac tgcaactacc tcttcatagt tcagaaacta acttgtcttt taatgaattt
     2161 tcagaaagct ttaaaacaac ttcccctgag aaaatgagca taagaaagcc acaggagaaa
     2221 tctgcagatg gaaaacaatt tttgcaaaat gataggaaaa atccaaagtt aggtaatact
     2281 caaactcttt tgagaaatga tgtttctgga cttttgagca gaactaaacg aaatattgtg
     2341 cctccagggt ggtattctgt atatgtaaca aataattatg tttttaaaaa atcccctaaa
     2401 gccaaaaaag tatccgaatc cacaacaaaa aacgatccag tgaaaaatat tcacattgaa
     2461 agctcacaca atatagacct aaacaaaatt gcaatgaatt ctaatttaca agtggttgtg
     2521 aagcgtttgg aagatacaat aagtatagcc aaaaaatcct ggaataatca gccattatca
     2581 gaaggatata aagcatccaa gaaattgata gaaattgatg gtaaagacca acatgctgac
     2641 agaaatatga ctcttactct aaatagaatg acatgcaaag aacagagctt atcaaaatct
     2701 gtggtagcat ccggcaatat tatcaatagt cactgcatgc ctacagtgga tttgaataac
     2761 aaaagacttg aaaatctgaa aaagtcatct attttagata tgggtcgctt gatttccagt
     2821 gttgaaaatg taccagcaaa atatgaaggt actgaaagtt catctgtttc caactattct
     2881 agtcctatca aactcatgtt tttatctgag gttaaaagta gtgaaggggt caaatatact
     2941 ttaacttcag ttggcacttc ccattcaaat gttgttctcc cttctgaaaa acctacaacc
     3001 catcacgtaa ctgaagaaaa aacagaaaca aatgaggata tctcaaatgc gaactctgaa
     3061 aattatcact ccagtcatta tgatactgat acttttcaaa gagaactaaa caaattcagt
     3121 catgcaaaag aaactgcagg atcctctaca atgtttatag gtgatataaa tagtgataag
     3181 ccacaagaag aacctaagga caattcaagc agtgctactg atccatcttt taaaagaaaa
     3241 ccaggtagac caaaaaaaat aggtccccaa gttgtgaaac agattaagcg accaattgga
     3301 agaccaccaa agcctaaaat tgatcaaaca aacatcactg tttgccagaa tgagcccttt
     3361 agtgctggaa ggaaaagccc agaatgtctc atatcagaag taaaagaagg tatttataaa
     3421 aagagtatta cagtaactgt tatttacgga aggtcaagaa gaactaaaag gcatgtttct
     3481 gaaggaaggg taaacataag caaccttatg tctttaaaca ataatgctgg tgattttcca
     3541 actgaatata atagtcccag aaatattagt gaagacaaaa ttgacttggg tgaaagaata
     3601 agtgctgtag caagcttgac tactgaaagt gagatcttgg ggtctggctt tgaatatgtg
     3661 agacccatca agaacaaatc tgtgatacct cagccttcca agaacattat tcgaccaaat
     3721 cagaagcctt tggcaataag taggaagcct ggtagaccgg caaaagtgaa aatctctggc
     3781 atatctgtga ctattaatag aacttcacct caggaaagag aagtaagtat tagcagctgc
     3841 ttgcctcctt tagaacagga taatacgtca ggaaaaaatc tgcctgaaga aaagtatgac
     3901 caacagtgca ctcaaatgga taaaataagg cacactgaag ctgatatatt taaaaatgga
     3961 tcaaaacgta tgattgctac tgtacctttg agacattcta ttagggatag aaaaccatct
     4021 ctgcatttct tacattcatt agcatcttct agctcactta tttatagaaa cgctctgctc
     4081 cataaatcat ataaactgca tttgcagaaa aataaaagtc agaaggaaaa acataggcac
     4141 tcaaagatga aaatagctta caaagatacc ccaagaaaca gactttcgcg gaatgcaaaa
     4201 aagtgtttgg aagataataa attagtacct atttctgaag tatccttgga tcctataatt
     4261 tcatcaaacc ctttgctcag gtggtgggct acttctgctt caaatgattc cttattagag
     4321 gaattaaaca atagatttga gcaaataaca aatgcttggg tgcaagtaag tggagatgaa
     4381 gctgaaaatt gtattcataa aaaaagagaa cacattgaaa atgatcattt caaagtagca
     4441 agccctttgg aaacttgtct tttagaactt gaagtttcac ctgtaaaaat gctttttcag
     4501 aaaaagtatg atttgaatga actctgtact tggtttatgc aaacaacaga aacacagtct
     4561 ctttcactag ttagaaaagc aaatgcccga aaccctttgg aagtaataaa taccagggga
     4621 atcaaattag ggaccaaata ttctgacttt aatgccagcc ccttcagaaa gcactttaaa
     4681 aaatttgcac tctcttctcc ttcaaaatca gcagagaaat tgcatatact gcataaagtg
     4741 gctaactctc cactcttaaa tgtgaaaagt aatttagcaa tagctagatt aaaaaggact
     4801 gagtttaaga ggttgcatca tgaaaggtgg aaaagagagg gaaagctgca caaccatgga
     4861 acagttgact ggaactctaa aaggagaaac ttaagatttt tctgccagaa ccaattttta
     4921 aataagactg agggagaaac aaatgctgac atcccactcc aaggaaaaag catagtagat
     4981 aatcagtgtg ttttgccacc tgagatcagg ggtgacttgc agcagagggt agtaatgcct
     5041 gacttcaaaa tacatgctag ttttgagaat aaatttaagt cagaagcaaa agagaatgga
     5101 acaaattgca gccaaaaaga ctttcaaaag ggaccaagac tagaaaatgt atgtcctaat
     5161 agttggaggt caaaaacctt aaaagactgt agaatatttt tgaggaagct caactgtctt
     5221 gaacacagaa atacttttaa gctaaataca atcatttact ctcctgaatc tactgacagt
     5281 ggaaatactc atcagactca tatggaagaa tcaaagcgct ttactttaag atctcattct
     5341 gctaggcaaa attcttttaa aaagcaatct aaagaaatag aaaatgctaa cacaaataat
     5401 ccttcagctg atgaatttgc tgaccatctt ggcaatagta aattaagcaa atgtgttaac
     5461 tttgacaaga atcctgatag ttttgaagtt cttagcaatt tgaacaaaag aaaaagacca
     5521 ccatggaaga tcacagaaat gtcaacaaaa agacataaac gacagtcttg caacagtgga
     5581 caaatggcaa actatttttc caaatcccta gcatgctaca agtagtaatg aactagtccg
     5641 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgagttatcg
     5701 ggcctcgcgg gatgcgc
//
