LOCUS       sequence-282322-       11521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0337
FEATURES             Location/Qualifiers
     source          1..11521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7378..7966
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7431..7451)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7849..7860)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8038..8271
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8349..8470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8386..8405)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8440..8459
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8510..8645
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8665..8684)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8666..8684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8694..8711)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8694..8710)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8703..8725)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8852..9307
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8939..8958)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9149..9170
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9333..9437
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9438..9506,9507..10298)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9656..9675)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10469..11057
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10958..10977
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11211..11228
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11345..11366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11381..11386,11387..11404,11405..11411)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11419..11435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11424..11446
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11443..11459
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11443..11459
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11478..11498
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11480..11498
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaag agcgaagact atccccatga
     4141 aactatggcg ccggatatcc acgaagaacg gcaatatcgc tgcgaagact gtgaccagct
     4201 ctttgaatct aaggctgaac tagcagatca ccaaaagttt ccatgcagta ctcctcactc
     4261 agcattttca atggttgaag aggactttca gcaaaaactc gaaagcgaga atgatctcca
     4321 agagatacac acgatccagg agtgtaagga atgtgaccaa gtttttcctg atttgcaaag
     4381 cctggagaaa cacatgctgt cacatactga agagagggaa tacaagtgtg atcagtgtcc
     4441 caaggcattt aactggaagt ccaatttaat tcgccaccag atgtcacatg acagtggaaa
     4501 gcactatgaa tgtgaaaact gtgccaaggt tttcacggac cctagcaacc ttcagcggca
     4561 cattcgctct cagcatgtcg gtgcccgggc ccatgcatgc ccggagtgtg gcaaaacgtt
     4621 tgccacttcg tcgggcctca aacaacacaa gcacatccac agcagtgtga agccctttat
     4681 ctgtgaggtc tgccataaat cctatactca gttttcaaac ctttgccgtc ataagcgcat
     4741 gcatgctgat tgcagaaccc aaatcaagtg caaagactgt ggacaaatgt tcagcactac
     4801 gtcttcctta aataaacaca ggaggttttg tgagggcaag aaccattttg cggcaggtgg
     4861 attttttggc caaggcattt cacttcctgg aaccccagct atggataaaa cgtccatggt
     4921 taatatgagt catgccaacc cgggccttgc tgactatttt ggcgccaata ggcatcctgc
     4981 tggtcttacc tttccaacag ctcctggatt ttcttttagc ttccctggtc tgtttccttc
     5041 cggcttgtac cacaggcctc ctttgatacc tgctagttct cctgttaaag gactatcaag
     5101 tactgaacag acaaacaaaa gtcaaagtcc cctcatgaca catcctcaga tactgccagc
     5161 tacacaggat attttgaagg cactatctaa acacccatct gtaggggaca ataagccagt
     5221 ggagctccag cccgagaggt cctctgaaga gaggcccttt gagaaaatca gtgaccagtc
     5281 agagagtagt gaccttgatg atgtcagtac accaagtggc agtgacctgg aaacaacctc
     5341 gggctctgat ctggaaagtg acattgaaag tgataaagag aaatttaaag aaaatggtaa
     5401 aatgttcaaa gacaaagtaa gccctcttca gaatctggct tcaataaata ataagaaaga
     5461 atacagcaat cattccattt tctcaccatc tttagaggag cagactgcgg tgtcaggagc
     5521 tgtgaatgat tctataaagg ctattgcttc tattgctgaa aaatactttg gttcaacagg
     5581 actggtgggg ctgcaagaca aaaaagttgg agctttacct tacccttcca tgtttcccct
     5641 cccatttttt ccagcattct ctcaatcaat gtacccattt cctgatagag acttgagatc
     5701 gttacctttg aaaatggaac cccaatcacc aggtgaagta aagaaactgc agaagggcag
     5761 ctctgagtcc ccctttgatc tcaccactaa gcgaaaggat gagaagccct tgactccagt
     5821 cccctccaag cctccagtga cacctgccac aagccaagac cagcccctgg atctaagtat
     5881 gggcagtagg agtagagcca gtgggacaaa gctgactgag cctcgaaaaa accacgtgtt
     5941 tgggggaaaa aaaggaagca acgtcgaatc aagacctgct tcagatggtt ccttgcagca
     6001 tgcaagaccc actcctttct ttatggaccc tatttacaga gtagagaaaa gaaaactaac
     6061 tgacccactt gaagctttaa aagagaaata cttgaggcct tctccaggat tcttgtttca
     6121 cccacaattc caactgcctg atcagagaac ttggatgtca gctattgaaa acatggcaga
     6181 aaagctagag agcttcagtg ccctgaaacc tgaggccagt gagctcttac agtcagtgcc
     6241 ctctatgttc aacttcaggg cgcctcccaa tgccctgcca gagaaccttc tgcggaaggg
     6301 aaaggagcgc tatacctgca gatactgtgg caagattttt ccaaggtctg caaacctaac
     6361 acggcacttg agaacccaca caggagagca gccttacaga tgcaaatact gtgacagatc
     6421 atttagcata tcttctaact tgcaaaggca tgttcgcaac atccacaata aagagaagcc
     6481 atttaagtgt cacttatgtg ataggtgttt tggtcaacaa accaatttag acagacacct
     6541 aaagaaacat gagaatggga acatgtccgg tacagcaaca tcgtcgcctc attctgaact
     6601 ggaaagtaca ggtgcgattc tggatgacaa agaagatgct tacttcacag aaattcgaaa
     6661 tttcattggg aacagcaacc atggcagcca atctcccagg aatgtggagg agagaatgaa
     6721 tggcagtcat tttaaagatg aaaaggcttt ggtgaccagt caaaattcag acttgctgga
     6781 tgatgaagaa gttgaagatg aggtgttgtt agatgaggag gatgaagaca atgatattac
     6841 tggaaaaaca ggaaaggaac cagtgacaag taatttacat gaaggaaacc ctgaggatga
     6901 ctatgaagaa accagtgccc tggagatgag ttgcaagaca tccccagtga ggtataaaga
     6961 ggaagaatat aaaagtggac tttctgctct agatcatata aggcacttca cagatagcct
     7021 caaaatgagg aaaatggaag ataatcaata ttctgaagct gagctgtctt cttttagtac
     7081 ttcccatgtg ccagaggaac ttaagcagcc gttacacaga aagtccaaat cgcaggcata
     7141 tgctatgatg ctgtcactgt ctgacaagga gtccctccat tctacatccc acagttcttc
     7201 caacgtgtgg cacagtatgg ccagggctgc ggcggaatcc agtgctatcc agtccataag
     7261 ccacgtatag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     7321 atcttgtgga aaggacgata atacccatgc ggtacaccct gcacgcgtta agtcgacaat
     7381 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7441 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7501 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7561 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7621 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     7681 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     7741 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     7801 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     7861 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     7921 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     7981 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     8041 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     8101 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8161 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8221 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     8281 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     8341 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8401 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8461 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     8521 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8581 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8641 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     8701 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     8761 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     8821 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     8881 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8941 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     9001 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9061 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9121 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9181 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     9241 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     9301 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9361 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9421 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9481 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9541 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9601 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9661 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9721 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9781 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9841 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9901 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9961 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    10021 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10081 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10141 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10201 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10261 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10321 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10381 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10441 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10501 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10561 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    10621 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10681 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10741 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10801 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10861 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10921 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10981 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    11041 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11101 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    11161 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    11221 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    11281 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    11341 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    11401 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    11461 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    11521 c
//
