LOCUS       sequence-282329-        3662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0346
FEATURES             Location/Qualifiers
     source          1..3662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggtgga caccgaggag aagcggcatc gcacgcggtc caaaggggtt
       61 cgagttcccg tggaaccagc catacaagag ctgttcagct gtcccacccc tggctgtgac
      121 ggcagtggtc atgtcagtgg caaatatgca agacacagaa gtgtatatgg ttgtcccttg
      181 gcgaaaaaaa gaaaaacaca agataaacag ccccaggaac ctgctcctaa acgaaagcca
      241 tttgccgtga aagcagacag ctcctcagtg gatgagtgtg acgacagtga tgggactgag
      301 gacatggatg agaaggagga ggatgagggg gaggagtact ccgaggacaa tgatgagcca
      361 ggggatgagg acgaggagga cgaggagggg gaccgggagg aggaggagga gatcgaggag
      421 gaggatgagg acgatgacga ggatggagaa gatgtggagg atgaagaaga ggaagaggag
      481 gaggaggagg aggaggaaga ggaagaagaa aacgaagacc atcaaatgaa ttgtcacaat
      541 actcgaataa tgcaagacac agaaaaggat gataacaata atgacgaata tgacaattac
      601 gatgaactgg tggccaagtc attgttaaac ctcggcaaaa tcgctgagga tgcagcctac
      661 cgggccagga ctgagtcaga aatgaacagc aatacctcca atagtctgga agacgatagt
      721 gacaaaaacg aaaacctggg tcggaaaagt gagttgagtt tagacttaga cagtgatgtt
      781 gttagagaaa cagtggactc ccttaaacta ttagcccaag gacacggtgt tgtgctctca
      841 gaaaacatga atgacagaaa ttatgcagac agcatgtcgc agcaagacag tagaaatatg
      901 aattacgtca tgttggggaa gcccatgaac aacggactca tggaaaagat ggtggaggag
      961 agcgatgagg aggtgtgtct gagcagtctg gagtgtttga ggaatcagtg cttcgacctg
     1021 gccaggaagc tcagtgagac caacccgcag gagaggaatc cgcagcagaa catgaacatc
     1081 cgtcagcatg tccggccaga agaggacttc cccggaagga cgccggacag aaactactcg
     1141 gacatgctga acctcatgcg gctggaggag cagttgagcc cccggtcgag agtgtttgcc
     1201 agctgtgcga aggaggatgg gtgtcatgag cgggacgacg ataccacctc tgtgaactcg
     1261 gacaggtctg aagaggtgtt cgacatgacc aaggggaacc tgaccctgct ggagaaagcc
     1321 atcgctttgg aaacggaaag agcaaaggcc atgagggaga agatggccat ggaagctggg
     1381 aggagggaca atatgaggtc atatgaggac cagtctccga gacaacttcc cggggaggac
     1441 agaaagccta aatccagtga cagccatgtc aaaaagccat actatggtaa agatccctca
     1501 agaacagaaa agaaagagag caagtgtcca acccccgggt gtgatggaac cggccacgta
     1561 actgggctgt acccacatca ccgcagcctg tccggatgcc cgcacaaaga tagggtccct
     1621 ccagaaatcc ttgccatgca tgaaagtgtc ctcaagtgcc ccactccggg ctgcacgggg
     1681 cgcgggcatg tcaacagcaa caggaactcc caccgaagcc tctccggatg cccgatcgct
     1741 gcagcagaga aactggccaa ggcacaggaa aagcaccaga gctgcgacgt gtccaagtcc
     1801 agccaggcct cggaccgcgt gctcaggcca atgtgctttg tgaagcagct ggagattcct
     1861 cagtatggct acagaaacaa tgtccccaca actacgccgc gttccaacct ggccaaggag
     1921 ctcgagaaat attccaagac ctcgtttgaa tacaacagtt acgacaacca tacttatggc
     1981 aagcgagcca tagctcccaa ggtgcaaacc agggatatat cccccaaagg atatgatgat
     2041 gcgaagcggt actgcaagga ccccagcccc agcagcagca gcaccagcag ctacgcgccc
     2101 agcagcagca gcaacctgag ctgcggcggg ggcagcagcg ccagcagcac gtgcagcaag
     2161 agcagcttcg actacacgca cgacatggag gcggcccaca tggcggccac cgccatcctc
     2221 aacctgtcca cgcgctgccg cgagatgccg cagaacctga gcaccaagcc gcaggacctg
     2281 tgcgccacgc ggaaccctga catggaggtg gatgagaacg ggaccctgga cctcagcatg
     2341 aacaagcaga ggccgcggga cagctgctgc cccatcctga cccctctgga gcccatgtcc
     2401 ccccagcagc aggcagtgat gaacaaccgg tgtttccagc tgggcgaggg cgactgctgg
     2461 gacttgcccg tagactacac caaaatgaaa ccccggagga tagacgagga cgagtccaaa
     2521 gacattactc cagaagactt ggacccattc caggaggctc tagaagaaag acggtatccc
     2581 ggggaggtga ccatcccaag tcccaaaccc aagtaccctc agtgcaagga gagcaaaaag
     2641 gacttaataa ctctgtctgg ctgccccctg gcggacaaaa gcattcgaag tatgctggcc
     2701 accagctccc aagaactcaa gtgccccacg cctggctgtg atggttctgg acatatcacc
     2761 ggcaattatg cttctcatcg gagcctttca ggttgcccaa gagcaaagaa aagtggtatc
     2821 aggatagcac agagcaaaga agataaagaa gatcaagaac ccatcaggtg tccggtcccc
     2881 gggtgcgacg gccagggcca catcactggg aagtacgcgt cccatcgcag cgcctccggg
     2941 tgccccttgg cggccaagag gcagaaagac gggtacctga atggctccca gttctcctgg
     3001 aagtcggtca agacggaagg catgtcctgc cccacgccag gatgcgacgg ctcaggccac
     3061 gtcagcggca gcttcctcac acaccgcagc ttgtcaggat gcccgagagc cacgtcagcg
     3121 atgaagaagg caaagctttc tggagagcag atgctgacca tcaaacagcg ggccagcaac
     3181 ggtatagaaa atgatgaaga aatcaaacag ttagatgaag aaatcaagga gctaaatgaa
     3241 tccaattccc agatggaagc cgatatgatt aaactcagaa ctcagattac cacgatggag
     3301 agcaacctga agaccatcga agaggagaac aaagtgattg agcagcagaa cgagtctctc
     3361 ctccacgagc tggcgaacct gagccagtct ctgatccaca gcctggctaa catccagctg
     3421 ccgcacatgg atccaatcaa tgaacaaaat tttgatgctt acgtgactac tttgacggaa
     3481 atgtatacaa atcaagatcg ttatcagagt ccagaaaata aagccctact ggaaaatata
     3541 aagcaggctg tgagaggaat tcaggtctag taatgaacta gtccgtaact tgaaagtatt
     3601 tcgatttctt ggctttatat atcttgtgga aaggacgaag atactgtagt gaacaacgtc
     3661 ga
//
