LOCUS       sequence-282335-        1400 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0348
FEATURES             Location/Qualifiers
     source          1..1400
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             442..462
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             472..492
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 accgccacca tgccgcggcc ggggaagagc tcgtacagcg accaaaaacc gccctactct
       61 tacatctcgc tgaccgccat ggcaatccag cactcggccg agaagatgct gccgctgagc
      121 gacatctaca agttcatcat ggagcgcttc ccctactacc gcgagcacac acagcgctgg
      181 cagaacagcc tgcgccacaa cctctccttc aacgactgct tcatcaagat tccgcggagg
      241 cccgaccagc ctggcaaggg tagcttctgg gcgctgcacc ccgactgcgg ggacatgttc
      301 gagaacggca gcttcctgcg gcgtcgcaag cgcttcaagg tgctgcgcgc cgaccatact
      361 cacttgcacg cgggaagcac caagagcgcg ccgggcgccg gtccgggagg gcaccttcac
      421 ccccatcacc accaccaccc ccaccaccac catcatcacc acgctgccgc acaccaccac
      481 catcaccacc acccacccca gccgccgccg ccgccgcccc cgccgccgcc gcacatggta
      541 cactatttcc atcagcaacc gcctactgct ccgcagccgc ctccgcacct cccgtcacag
      601 cccccgcagc aaccgcccca gcagtcgcag cctcagcagc cgtctcaccc cggcaagatg
      661 caggaggcgg cggccgtggc ggcggcggcg gcggcggccg cggcagccgc ggtgggcagc
      721 gtgggacgcc tgtctcagtt cccaccctac gggctgggct cggccgccgc cgctgccgcc
      781 gcggccgcgg cgtccacgtc aggcttcaag cacccctttg ccattgagaa cattattggc
      841 cgggactaca agggcgtgct gcaggctgga gggctgccct tggcgtccgt catgcaccac
      901 ctgggctacc ccgtgcccgg ccagcttggc aacgtcgtca gctccgtgtg gccgcacgtt
      961 ggcgtcatgg attcggtggc cgccgccgcg gccgccgcag ccgcagccgg agtccctgta
     1021 ggcccggagt atggggcctt cggggtcccg gtcaagtccc tgtgccactc ggcaagccag
     1081 agcctgcctg ccatgccggt gcccatcaag cccacgcctg cgctgccgcc cgtgtccgcg
     1141 ctgcagccgg ggctcactgt ccccgcggct tcgcagcagc ctccggcgcc atccaccgtg
     1201 tgctccgcgg ccgcggcctc gcccgttgcc tctctgctgg agcccacagc ccctacctcg
     1261 gccgaaagca agggcggctc cttgcactcg gtgctagtgc actcctagta atgaactagt
     1321 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaggcg
     1381 cggtcccgga atgctacctc
//
