LOCUS       sequence-282338-        1958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0350
FEATURES             Location/Qualifiers
     source          1..1958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcgcgc ggtagagcct tggggcccag atctccaccg cccggaggag
       61 agggagccac agagaggcgc ccgcacaggt ctagggagtg agaacgtgat ttctcagccg
      121 aatgagtttg aacatacccc acaggaagat gacttggggt tcaaggaaga agatttggct
      181 ccagatcatg aagtaggaaa tgcctctctc aaacctgaag gcatccagaa ctgggatgac
      241 ttatgggtcc agagagaggg tctaggaaag cctcagcctc gggacagagg cccccggctc
      301 ctgggtgaac cacgctgggg ccaggctagt agtgatcggg ccgctgtgtg tggtgagtgt
      361 ggcaaaagct tcaggcagat gtcagatctg gtgaaacacc agcggaccca cacaggggag
      421 aaaccctaca agtgtggggt ctgtggcaag ggctttgggg atagctctgc ccggatcaaa
      481 caccagcgga ctcatagtgg ggagaagccc tatagagccc ggccaccagc ccagggtccc
      541 ccaaagattc ctcggtcccg gatccctgct ggtgagcgcc ccactatctg tggtgaatgt
      601 ggcaagagct tccggcagag ttctgacctg gtgaaacacc agcggacaca cactggtgag
      661 aagccctaca agtgtggcat atgtggcaag ggctttggcg acagttccgc ccgcatcaag
      721 caccagcgga cacaccgggg ggagcagccc ccccgaccag tggtgccccg acggcagcca
      781 tctcgggcag ccacggcagc tacccaggga ccgaaggccc aggacaagcc atatatctgc
      841 actgattgcg gcaagaggtt tgtgctcagc tgcagcctcc tgagtcacca gcgtagtcac
      901 ttggggccca agccctttgg ctgtgatgtg tgtggaaagg agtttgcccg gggatccgac
      961 ctggtgaagc acctgcgggt gcacacgggt gagaagccct acctctgccc agagtgcggc
     1021 aaaggtttcg cggacagctc cgcccgagtc aaacacctcc gcacccacag tggcgagagg
     1081 ccccatgcct gcccggaatg cgaccgtacc ttcagcctca gctccaccct tcttcgccac
     1141 cgcctcactc acatggagcc ccaggacttc agcttcccag gctatcccct acccgctctg
     1201 atccccagcc cacccccacc tcctctgggc accagccccc cgctgacacc tcgaagtccc
     1261 tcacactcgg gtgagccttt tggcctgcct ggcttggagc cagagcctgg gggcccacag
     1321 gctggggagc cacccccacc actggcgggc gacaagcccc acaagtgccc tgagtgtggc
     1381 aagggcttcc gccgaagctc tgacctggtg aaacaccatc gtgtgcacac aggggagaaa
     1441 ccctacctct gtcctgaatg cggcaagggt tttgctgaca gctcagcccg agtcaagcac
     1501 ctccgcaccc accgtggtga acgggcccgg ccaccaccac catccactct gctgcggcca
     1561 cataacccac ctggcccagt acccatggcc cctcgacccc gagttcgggc ccagccttct
     1621 ggacccagcc agccccacgt gtgtggcttc tgtgggaagg agttcccccg gagctcagat
     1681 ctggtcaaac acaggcgtac acacacgggg gagaagccat acaagtgtgc agagtgtggc
     1741 aagggttttg gtgacagttc tgcccgcatc aagcaccagc gtgggcacct ggtcctgacg
     1801 ccctttggga taggggatgg tagggcaagg cccctcaagc aggaggcagc aacaggactg
     1861 gaatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1921 tgtggaaagg acgagccatc gttcgcgatt gcgataag
//
