LOCUS       sequence-282347-        1418 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0398
FEATURES             Location/Qualifiers
     source          1..1418
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgctgt gtctccgacc accagatgcc aggaatcagt gacattcgaa
       61 gacgttgccg tggttttcac agatgaagag tggagtcgtc tggtccccat acagagggac
      121 ctctacaagg aggtgatgct ggagaactat aacagcattg tgtcattggg ccttccagtt
      181 cctcaacctg atgtgatttt ccaattgaag agaggggaca agccgtggat ggtagatctt
      241 catgggtctg aggagagaga atggccagag agtgtctctc tagcccagga aaaaaactgc
      301 atctgttttc tgtttgttcc agactgggaa actaagcctg agattcacga tgcttcagac
      361 aaaaaatcag aaggatcatt gagggaatgc cttggaaggc aaagtcctct gtgtcctaaa
      421 tttgaagttc atacacccaa tggcaggatg ggaacagaaa agcaaagccc ttcaggggag
      481 actcgtaaga aatccctctc ccgggacaaa ggcttgcggc gacggtcagc cctgtccagg
      541 gaaattctca ctaaagagag acaccaggaa tgcagtgact gtgggaagac cttttttgac
      601 cactcatccc tcacccgcca tcagaggact cacactgggg agaagcccta cgactgccgc
      661 gagtgtggga aagccttcag ccacaggagc agcctcagca gacatctgat gtcacacact
      721 ggggagagcc cctacgagtg cagtgtgtgc tcaaaagcct tctttgaccg ttcgtcccta
      781 actgtccatc agcgaattca cactggagag aaaccctttc agtgcaacga gtgtggaaaa
      841 gccttttttg accgttcatc ccttactcga caccagagaa ttcacactgg agaaagtcct
      901 tatgaatgtc atcagtgtgg gaaagccttt agccagaaaa gtattcttac tcgccatcag
      961 ctaatccaca ctggcaggaa gccttatgag tgtaacgagt gcgggaaagc tttctatggt
     1021 gtctcgtctc tgaatagaca tcagaaagct catgctgggg accctcgcta tcagtgtaac
     1081 gagtgtggca aagctttctt tgaccgctca tcccttacac agcatcagaa gatccacact
     1141 ggagacaagc catatgaatg cagcgaatgc gggaaagcct ttagccagcg gtgccggctc
     1201 acgcggcatc agcgtgtcca cacgggagag aagccctttg aatgcactgt gtgtgggaaa
     1261 gttttcagtt caaaatcttc tgttattcaa catcaacggc gttacgccaa acagggaata
     1321 gactagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1381 tgtggaaagg acgaccttaa gcggtgtgtt cttgccat
//
