LOCUS       sequence-28235-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Qian F, Boletta A, Bhunia AK, Xu H, Liu L, Ahrabi AK, Watnick TJ, 
            Zhou F, Germino GG
  TITLE     Cleavage of polycystin-1 requires the receptor for egg jelly domain 
            and is disrupted by human autosomal-dominant polycystic kidney 
            disease 1-associated mutations.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Dec 24. 99(26):16981-6.
  PUBMED    12482949
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Dec 24. 99(26):16981-6."
COMMENT     Sequence Label: E3020X
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             488..511
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    571..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tgggcctcag caggtcaagg agttccgcca caaagtccgc tttgaaggga tggagccgct
       61 gccctctcgc tcctccaggg gctccaaggt atccccggat gtgcccccac ccagcgctgg
      121 ctccgatgcc tcgcacccct ccacctcctc cagccagctg gatgggctga gcgtgagcct
      181 gggccggctg gggacaaggt gtgagcctga gccctcccgc ctccaagccg tgttcgaggc
      241 cctgctcacc cagtttgacc gactcaacca ggccacagag gacgtctacc agctggagca
      301 gcagctgcac agcctgcaag gccgcaggag cagccgggcg cccgccggat cttcccgtgg
      361 cccatccccg ggcctgcggc cagcactgcc cagccgcctt gcccgggcca gtcggggtgt
      421 ggacctggcc actggcccca gcaggacacc ccttcgggcc aagaacaagg tccaccccag
      481 cagcactgac tacaaggacg acgatgacaa gtgatcaggt agcttatcga taccgtcgac
      541 ccgggcggcc gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
      721 ttcaggttca gggnggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
      781 ggtaaaatcg ataaggatca attgcctttt ttttttaatt tttattttaa ttttatt
//
