LOCUS       sequence-282376-       12097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12097)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0545
FEATURES             Location/Qualifiers
     source          1..12097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7954..8542
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8007..8027)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8425..8436)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8614..8847
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8925..9046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8962..8981)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9016..9035
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9086..9221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9241..9260)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9242..9260)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9270..9287)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9270..9286)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9279..9301)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9428..9883
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9515..9534)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9725..9746
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9909..10013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10014..10082,10083..10874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10232..10251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11045..11633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11534..11553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11787..11804
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11921..11942
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11957..11962,11963..11980,11981..11987)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11995..12011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12000..12022
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12019..12035
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12019..12035
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12054..12074
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12056..12074
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag acgcgggcgg cagaaaacac
     4141 ggccatcttt atgtgtaaat gttgtaacct cttctcacca aatcagtcgg aactcctctc
     4201 ccacgtttca gagaagcaca tggaagaagg ggttaatgtt gatgagatta ttattcccct
     4261 taggcctctg agtacacctg aaccccccaa ctcaagcaaa accggagatg agtttttggt
     4321 catgaagagg aagagaggca ggcctaaggg gtccacgaag aagtccagca cagaagagga
     4381 gctggcagaa aacatcgtga gtccgactga ggacagcccg ctggctccgg aggaagggaa
     4441 cagcctgcct ccaagcagct tggagtgtag caagtgctgt cggaagttct ccaacacgcg
     4501 ccagctgcgg aagcacatct gcattatcgt gctgaatttg ggtgaggagg aaggagaagc
     4561 aggtaacgag tctgaccttg aactagaaaa gaagtgtaag gaagatgatc gggaaaaagc
     4621 ctcgaaaaga ccacggtcac agaaaacaga gaaagtccag aagatctcag gaaaggaggc
     4681 cagacagctt tctggggcga agaaacccat cataagtgtg gttttaactg cacacgaagc
     4741 aattccaggt gctaccaaga ttgtgccagt ggaggctggg ccccctgaaa caggagctac
     4801 aaattctgag accacttcag cagacctggt gcctcggaga ggctaccagg aatacgccat
     4861 tcagcagaca ccttatgagc aaccaatgaa gtcaagcagg ctaggtccca ctcagctcaa
     4921 aatcttcact tgtgaatact gcaacaaggt cttcaagttc aagcactcgc tgcaggccca
     4981 cctgaggatc cacaccaatg aaaagccata caagtgcccc cagtgcagct atgccagtgc
     5041 catcaaggcc aacctcaatg tgcacctgcg caagcacact ggagagaagt tcgcctgcga
     5101 ctattgctcg ttcacctgcc tgagcaaggg ccacctcaag gtgcacatcg agcgagtgca
     5161 caagaagatc aagcagcact gccgcttctg caagaagaag tactctgacg tcaagaacct
     5221 catcaagcac atccgagacg cgcatgaccc acaggacaag aaggtcaaag aggccttgga
     5281 cgagctctgc ctgatgacga gggagggcaa gcggcagctg ctctatgact gccacatctg
     5341 tgagcgcaag ttcaagaacg agctggaccg tgaccgccat atgctggtcc acggagacaa
     5401 gtggcctttt gcctgtgagc tctgtggcca tggggccacc aagtaccagg cgctggaact
     5461 gcatgtcagg aagcacccct tcgtgtacgt ctgtgccgtc tgccgcaaga agttcgtcag
     5521 ctccatcagg ctgcgcaccc acatcaaaga ggtgcacggg gctgcccagg aggccttggt
     5581 cttcaccagt tccatcaacc agagcttctg cctcctggaa cctggtgggg acatccagca
     5641 agaagctctg ggggaccagc tacagctggt ggaagaggag tttgccctcc agggcgtgaa
     5701 tgcactcaag gaagaggcct gtcctgggga cactcagctg gaggagggcc ggaaggagcc
     5761 ggaggcccct ggggaaatgc ctgccccagc tgtgcacctg gcctccccgc aggccgaaag
     5821 cacagccctg ccaccctgtg agctggaaac caccgtggtc tcctcctcag acctgcattc
     5881 tcaagaggtg gtttcagatg attttttgtt gaaaaatgat acctcctccg cagaggctca
     5941 tgctgctcct gagaagcccc cagacatgca gcacagaagc tcagtccaga cgcaaggtga
     6001 agtgatcaca ctactgctgt ccaaggccca gagtgctggg tcagatcagg aaagccatgg
     6061 cgcccagagc cccctagggg aagggcagaa catggctgtg ctttcagctg gtgacccaga
     6121 tcccagcagg tgtctcaggt caaacccagc tgaggcctca gacctcctcc ctccagtagc
     6181 tggtggtggg gacaccatca cacatcagcc tgactcttgc aaagctgccc ctgagcaccg
     6241 gtcaggcatc accgctttca tgaaggtcct gaacagttta cagaagaagc aaatgaacac
     6301 cagcttgtgt gagcggatcc ggaaggttta tggagacctg gagtgtgaat actgtggcaa
     6361 acttttttgg taccaagtgc attttgatat gcatgtccgc acccacaccc gggaacatct
     6421 gtattattgc tctcagtgtc attattcttc catcaccaaa aactgcctta aacgccacgt
     6481 aattcagaaa cacagtaaca tcttgctgaa gtgtcccacc gatggctgtg actactcaac
     6541 tccagataaa tataagctac aggcacatct taaagttcac acagcactgg acaaaaggag
     6601 ttattcttgt cctgtttgtg aaaagtcttt ttcagaggat cgattgataa agtcacatat
     6661 caagaccaac catcctgagg tctccatgag caccatttct gaggttctcg ggaggagggt
     6721 tcagctgaaa gggctaattg gaaagagagc catgaaatgc ccatattgtg acttttattt
     6781 catgaagaat ggctcagacc ttcagcgtca tatttgggct catgaaggtg tgaagccctt
     6841 caagtgttct ttgtgtgagt atgcaactcg tagcaagagt aacctcaagg ctcatatgaa
     6901 tcgtcacagc actgagaaaa cccacctatg tgacatgtgt ggcaagaaat tcaaatcaaa
     6961 agggacactg aaaagtcaca aactccttca cactgcagat gggaagcagt ttaagtgcac
     7021 ggtgtgtgac tacacagcgg cccagaagcc acagctgctg cggcacatgg aacagcatgt
     7081 ctccttcaag cctttccgct gtgcccattg ccattactcc tgcaacatat ctggctctct
     7141 gaagcggcac tacaacagga agcaccctaa tgaggagtat gccaacgtgg gcaccgggga
     7201 gctggcagcg gaggtgctca tccagcaagg tggtttgaag tgtcctgttt gcagctttgt
     7261 atatggcacc aaatgggagt tcaataggca cttgaagaac aaacatggct tgaaggtggt
     7321 ggaaattgat ggagacccca agtgggagac agcaacagaa gctcctgagg agccctccac
     7381 ccagtatctc cacatcacag aggccgaaga agacgttcaa gggacacagg cagcggtggc
     7441 cgcgctccag gacctgagat acacctctga gagtggcgac cgactggacc ccacggccgt
     7501 gaacatcctg cagcagatca ttgagctggg cgccgagacc catgacgcca ctgcccttgc
     7561 ctcggtggtt gccatggcac cagggacggt gactgtggtt aagcaggtca ccgaggagga
     7621 gcccagctcc aaccacacgg tcatgatcca ggagacggtc cagcaagcgt ccgtggagct
     7681 tgccgagcag caccacctgg tggtgtcctc cgacgacgtg gagggcattg agacggtgac
     7741 tgtctacacg cagggcgggg aggcctcgga gttcatcgtc tacgtgcagg aggccatgca
     7801 gcctgtggag gagcaggctg tggagcagcc ggcccaggaa ctctagtaat gaactagtcc
     7861 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgatctacg
     7921 tttcagggtg tagttgacac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     7981 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8041 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8101 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8161 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8221 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     8281 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8341 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     8401 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8461 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     8521 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     8581 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     8641 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8701 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8761 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8821 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     8881 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     8941 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9001 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     9061 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9121 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9181 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     9241 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     9301 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     9361 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     9421 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     9481 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     9541 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     9601 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     9661 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     9721 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     9781 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     9841 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     9901 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9961 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    10021 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    10081 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    10141 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    10201 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    10261 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    10321 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    10381 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    10441 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    10501 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    10561 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    10621 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    10681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    10741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    10801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    10861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    10921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    11041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    11101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    11161 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    11221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    11281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    11341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    11401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    11461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    11521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    11581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    11641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    11701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    11761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    11821 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    11881 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    11941 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    12001 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    12061 accctcacta aagggaacaa aagctggagc tgcaagc
//
