LOCUS       sequence-282377-        3833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0545
FEATURES             Location/Qualifiers
     source          1..3833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagacgcg ggcggcagaa aacacggcca tctttatgtg taaatgttgt
       61 aacctcttct caccaaatca gtcggaactc ctctcccacg tttcagagaa gcacatggaa
      121 gaaggggtta atgttgatga gattattatt ccccttaggc ctctgagtac acctgaaccc
      181 cccaactcaa gcaaaaccgg agatgagttt ttggtcatga agaggaagag aggcaggcct
      241 aaggggtcca cgaagaagtc cagcacagaa gaggagctgg cagaaaacat cgtgagtccg
      301 actgaggaca gcccgctggc tccggaggaa gggaacagcc tgcctccaag cagcttggag
      361 tgtagcaagt gctgtcggaa gttctccaac acgcgccagc tgcggaagca catctgcatt
      421 atcgtgctga atttgggtga ggaggaagga gaagcaggta acgagtctga ccttgaacta
      481 gaaaagaagt gtaaggaaga tgatcgggaa aaagcctcga aaagaccacg gtcacagaaa
      541 acagagaaag tccagaagat ctcaggaaag gaggccagac agctttctgg ggcgaagaaa
      601 cccatcataa gtgtggtttt aactgcacac gaagcaattc caggtgctac caagattgtg
      661 ccagtggagg ctgggccccc tgaaacagga gctacaaatt ctgagaccac ttcagcagac
      721 ctggtgcctc ggagaggcta ccaggaatac gccattcagc agacacctta tgagcaacca
      781 atgaagtcaa gcaggctagg tcccactcag ctcaaaatct tcacttgtga atactgcaac
      841 aaggtcttca agttcaagca ctcgctgcag gcccacctga ggatccacac caatgaaaag
      901 ccatacaagt gcccccagtg cagctatgcc agtgccatca aggccaacct caatgtgcac
      961 ctgcgcaagc acactggaga gaagttcgcc tgcgactatt gctcgttcac ctgcctgagc
     1021 aagggccacc tcaaggtgca catcgagcga gtgcacaaga agatcaagca gcactgccgc
     1081 ttctgcaaga agaagtactc tgacgtcaag aacctcatca agcacatccg agacgcgcat
     1141 gacccacagg acaagaaggt caaagaggcc ttggacgagc tctgcctgat gacgagggag
     1201 ggcaagcggc agctgctcta tgactgccac atctgtgagc gcaagttcaa gaacgagctg
     1261 gaccgtgacc gccatatgct ggtccacgga gacaagtggc cttttgcctg tgagctctgt
     1321 ggccatgggg ccaccaagta ccaggcgctg gaactgcatg tcaggaagca ccccttcgtg
     1381 tacgtctgtg ccgtctgccg caagaagttc gtcagctcca tcaggctgcg cacccacatc
     1441 aaagaggtgc acggggctgc ccaggaggcc ttggtcttca ccagttccat caaccagagc
     1501 ttctgcctcc tggaacctgg tggggacatc cagcaagaag ctctggggga ccagctacag
     1561 ctggtggaag aggagtttgc cctccagggc gtgaatgcac tcaaggaaga ggcctgtcct
     1621 ggggacactc agctggagga gggccggaag gagccggagg cccctgggga aatgcctgcc
     1681 ccagctgtgc acctggcctc cccgcaggcc gaaagcacag ccctgccacc ctgtgagctg
     1741 gaaaccaccg tggtctcctc ctcagacctg cattctcaag aggtggtttc agatgatttt
     1801 ttgttgaaaa atgatacctc ctccgcagag gctcatgctg ctcctgagaa gcccccagac
     1861 atgcagcaca gaagctcagt ccagacgcaa ggtgaagtga tcacactact gctgtccaag
     1921 gcccagagtg ctgggtcaga tcaggaaagc catggcgccc agagccccct aggggaaggg
     1981 cagaacatgg ctgtgctttc agctggtgac ccagatccca gcaggtgtct caggtcaaac
     2041 ccagctgagg cctcagacct cctccctcca gtagctggtg gtggggacac catcacacat
     2101 cagcctgact cttgcaaagc tgcccctgag caccggtcag gcatcaccgc tttcatgaag
     2161 gtcctgaaca gtttacagaa gaagcaaatg aacaccagct tgtgtgagcg gatccggaag
     2221 gtttatggag acctggagtg tgaatactgt ggcaaacttt tttggtacca agtgcatttt
     2281 gatatgcatg tccgcaccca cacccgggaa catctgtatt attgctctca gtgtcattat
     2341 tcttccatca ccaaaaactg ccttaaacgc cacgtaattc agaaacacag taacatcttg
     2401 ctgaagtgtc ccaccgatgg ctgtgactac tcaactccag ataaatataa gctacaggca
     2461 catcttaaag ttcacacagc actggacaaa aggagttatt cttgtcctgt ttgtgaaaag
     2521 tctttttcag aggatcgatt gataaagtca catatcaaga ccaaccatcc tgaggtctcc
     2581 atgagcacca tttctgaggt tctcgggagg agggttcagc tgaaagggct aattggaaag
     2641 agagccatga aatgcccata ttgtgacttt tatttcatga agaatggctc agaccttcag
     2701 cgtcatattt gggctcatga aggtgtgaag cccttcaagt gttctttgtg tgagtatgca
     2761 actcgtagca agagtaacct caaggctcat atgaatcgtc acagcactga gaaaacccac
     2821 ctatgtgaca tgtgtggcaa gaaattcaaa tcaaaaggga cactgaaaag tcacaaactc
     2881 cttcacactg cagatgggaa gcagtttaag tgcacggtgt gtgactacac agcggcccag
     2941 aagccacagc tgctgcggca catggaacag catgtctcct tcaagccttt ccgctgtgcc
     3001 cattgccatt actcctgcaa catatctggc tctctgaagc ggcactacaa caggaagcac
     3061 cctaatgagg agtatgccaa cgtgggcacc ggggagctgg cagcggaggt gctcatccag
     3121 caaggtggtt tgaagtgtcc tgtttgcagc tttgtatatg gcaccaaatg ggagttcaat
     3181 aggcacttga agaacaaaca tggcttgaag gtggtggaaa ttgatggaga ccccaagtgg
     3241 gagacagcaa cagaagctcc tgaggagccc tccacccagt atctccacat cacagaggcc
     3301 gaagaagacg ttcaagggac acaggcagcg gtggccgcgc tccaggacct gagatacacc
     3361 tctgagagtg gcgaccgact ggaccccacg gccgtgaaca tcctgcagca gatcattgag
     3421 ctgggcgccg agacccatga cgccactgcc cttgcctcgg tggttgccat ggcaccaggg
     3481 acggtgactg tggttaagca ggtcaccgag gaggagccca gctccaacca cacggtcatg
     3541 atccaggaga cggtccagca agcgtccgtg gagcttgccg agcagcacca cctggtggtg
     3601 tcctccgacg acgtggaggg cattgagacg gtgactgtct acacgcaggg cggggaggcc
     3661 tcggagttca tcgtctacgt gcaggaggcc atgcagcctg tggaggagca ggctgtggag
     3721 cagccggccc aggaactcta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     3781 tggctttata tatcttgtgg aaaggacgat ctacgtttca gggtgtagtt gac
//
