LOCUS       sequence-282389-        1730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0582
FEATURES             Location/Qualifiers
     source          1..1730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgaccc gaggtatgga atgtgtcctc tcaagggagc aagtggatgc
       61 cctggggctg agaggagtct tctggtgcag tcttattttg aaaaggggcc attgacgttt
      121 agggatgtgg ccatagaatt ctctctggag gagtggcaat gcctggacag tgctcagcag
      181 ggtttgtata ggaaagtgat gttagagaac tacagaaacc tggtcttctt ggcaggtatt
      241 gctctcacta agccagacct gatcacctgt ctggagcaag gaaaagagcc ctggaatata
      301 aagagacatg agatggtagc caaaccccca gttatatgtt ctcattttcc ccaagacctt
      361 tgggcagagc aggacattaa agattctttt caagaagcga ttctgaaaaa atatggaaaa
      421 tatggacatg acaatttaca gttacaaaaa ggctgtaaaa gtgtggatga gtgtaaagtg
      481 cacaaagaac atgataacaa attaaaccag tgtttgataa ctacccagag caacatattt
      541 caatgtgatc catctgcaaa agtctttcat acattttcaa attcaaacag acataagata
      601 agacatacta gaaagaaacc tttcaaatgt aaaaaatgtg aaaaatcatt ttgcatgctt
      661 ttacacctaa ctcaacataa aagatttcat attacagaga attcctacca atgtaaagat
      721 tgtggcaaag ccttcaactg gttctcaacc cttactacac acaggagaat tcatactgga
      781 gagaaaccct acaaatgtga agaatgtggg aaagcattta accggtcctc acaccttact
      841 acacataaga taattcatac tggagagaaa ccatacagat gtgaagaatg tgggaaagct
      901 tttaaccggt cttcacacct tactacacat aaaagaattc atactggagt gaaaccctac
      961 aaatgtacag aatgtggcaa agcttttaac cggtcctcac accttactac acacaggata
     1021 attcatactg gagagaaacc ctacaaatgt gaagaatgtg gcaaagcctt taaccagtcc
     1081 tcaaccctta ctacacataa gataactcat gctggagaga aaccttacaa atgtgaagaa
     1141 tgtggcaaag ctttttaccg attctcatac cttactaaac ataagacaag tcatactgga
     1201 gagaaattct acaaatgtga agaatgcggc aaaggcttta actggtcctc agccctcact
     1261 aaacataaga gaattcatac tggagagaaa ccctacaaat gtgaagaatg tggcaaagct
     1321 tttaatgagt cctcaaacct tactacccat aagatgattc atactggaga gaaaccctac
     1381 aaatgtgacg aatgtggcaa agcctttaac cggtcctcac aactaactgc acataagatg
     1441 attcatactg gagagaaacc ctacaaatgt gaggaatgtg gcaaagcttt taaccgatcc
     1501 tcaaccctta ctaaacataa gataactcat actggagaga aatcttacaa atgggaagaa
     1561 tgtggtaaag actttaacca gtccctaagc cttattaaac aaaataactc atactggaga
     1621 gaaaccctac aaatgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1681 ctttatatat cttgtggaaa ggacgaacga ggtcattaat cgctctcgcg
//
