LOCUS       sequence-282395-        1580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0590
FEATURES             Location/Qualifiers
     source          1..1580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgcgaa gctgcgagcg catcaggtgg acgtggaccc ggacttcgcg
       61 ccgcagagcc ggccgcgctc gtgtacctgg cccctgccgc agcctgactt ggccggcgac
      121 gaggacggag cgctgggcgc aggggtggcc gagggcgccg aggactgcgg gccggagcgc
      181 cgggctacgg ccccggcgat ggccccagcg ccgcccctgg gcgcggaggt cggaccgctg
      241 cggaaagcga agagctctcg gcggaacgcg tgggggaacc tgtcctacgc cgacctcatc
      301 accaaagcca tcgagagcgc cccggacaag cggctcacgc tctcgcagat ctacgactgg
      361 atggtccgtt acgtgcccta cttcaaggat aaaggcgaca gcaacagctc ggccggctgg
      421 aagaactcca tccggcacaa cctgtcgctg cacacccgtt tcatccgcgt gcagaacgag
      481 ggcaccggca agagttcgtg gtggatgctg aaccccgagg gcggaaagac agggaagacc
      541 ccgcggcgca gggccgtgtc catggacaac ggggccaagt tcctgcgcat caagggcaag
      601 gcgagcaaga agaagcagct gcaggcgccc gagcgaagcc cggacgacag ctccccgagt
      661 gcgcccgccc cggggccggt gcctgccgca gccaagtggg ccgccagccc cgcctcgcac
      721 gccagcgacg actacgaggc ttgggccgac ttccgcggcg gcgggagacc cctgctcggg
      781 gaggcggccg agctggagga cgacgaggcc ctggaggccc tggcgccatc atcgccgctc
      841 atgtacccaa gccccgccag cgcgctgtcg ccggcgctgg gctcgcgctg tccgggtgag
      901 ctgccccgcc tggccgagct gggaggcccg ctgggcctgc acggcggcgg cggcgcgggg
      961 ctgcccgagg gcctgctgga cggcgcgcag gacgcgtacg ggccgcgggc ccgcgccagg
     1021 cccggcccgg tgctgggtgc gccgggggag ctggcgctgg cgggcgcagc cgccgcctac
     1081 cccggcaaag gggcggcccc gtacgcgccg cccgcgccct cgcgcagtgc cttagcccac
     1141 cccatcagcc ttatgacgct gcccggcgag gcgggcgccg cgggcctggc accgccgggc
     1201 cacgccgccg ccttcggggg cccgcccggc ggcctcctgc tggacgctct gccggggccc
     1261 tacgctgccg ccgccgccgg gccgctgggc gccgcgcccg accgcttccc ggccgacctg
     1321 gacctcgaca tgttcagcgg gagcctcgag tgcgacgtgg agtccatcat cctcaacgac
     1381 ttcatggaca gcgacgaaat ggacttcaac ttcgattcgg ccctgcctcc gccgccgccg
     1441 ggcctggccg gggccccgcc ccccaaccag agctgggtgc cgggctagta atgaactagt
     1501 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgacttt
     1561 cgatgatata gcggttagcc
//
